Microscope Basics T. Trimpe 2005 http://sciencespot.net/

Ocular lens (Eyepiece) Body Tube Nosepiece Arm Objectives Stage Stage Clips Coarse Adjustment Diaphragm Fine Adjustment Light Always carry a microscope with one hand holding the arm and one hand under the base. Base

Fill in the table on your worksheet. What’s my power? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective. What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.

RED =4x ( DO NOT USE THIS ONE) E8 microscopes Have THREE objective lenses: RED =4x ( DO NOT USE THIS ONE) Yellow = 10x = low power Blue = 40x = high power

Have THREE objective lenses: E8 microscopes Have THREE objective lenses: BAND COLOR OBJECTIVE LENS POWER EYEPIECE LENS POWER TOTAL POWER OF MAGNIFICATION RED DO NOT USE (4x) 10 YELLOW (low power) (10x) BLUE (high power) (40x)

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

Microscope Protocol Always begin, end and store a microscope with the lowest power objective in place for compound microscope lower the stage all the way between uses using the large coarse adjustment knob Always begin, end and store a clean microscope Clean all lenses and the stage with lens paper only Always begin, end and store a covered microscope with a wrapped cord – do not wrap cord around the microscope, use the ties. Always unplug a microscope by grasping the plug not the cord!!!

How to Start Each Time Look at microscope from the side Lower stage = raise objective all the way… Rotate nosepiece to LOWEST power (10x)….. wait for the click! Look through eyepiece – turn COARSE ADJUSTMENT until you get an image DON’T TOUCH COARSE ADJUSTMENT AFTER THIS! Turn FINE ADJUSTMENT knob slightly until image comes in to focus.

Let’s give it a try ... 1 – Turn on the microscope and then rotate the nosepiece to (LOW POWER = x10 EVERYONE) 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 3 – Once you have the image in view, rotate the nosepiece to view it under high power. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it without breaking it! 4 – When you are done, turn off the microscope and put up the slides you used away in the storage container.

How to label your drawing Put a title here & underline it Today your have a square to represent your field of view Make horizontal lines with a ruler to the right for your labels Write your total magnification here

Tips for good microscopic drawings Use pencil - you can erase and shade areas. Use only white printer paper. Use a circle for the outline of the field of view. Specimens should be drawn to scale - that is, the size of your drawing should reflect the size that you view them in the microscope. Write the title and magnification Label your drawing. All labels should be in printing- use horizontal lines drawn with a ruler. Avoid crossing label lines at all costs. Lightly color drawings when appropriate to enhance your drawings or to highlight a specific cell part.

Microscope drawings https://youtu.be/nQ47Zx9Sak0

You do not need to use the stage clips when viewing wet-mount slides! How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

Mrs. Howard’s microscopes GRAY WHITE Have THREE objective lenses: Green=10x= low power Yellow=43x= high power RED =100x (DO NOT USE!!! It is for oil immersion slides!) Have THREE objective lenses: RED =4x (YOU CAN SKIP THIS ONE) Yellow =10x = low power Blue =40x = high power

Mrs. Howard’s microscope rules: Clean up after yourself Return all supplies the way you found them Push your chairs in No slides left on microscopes Covers on the large white scopes Cords wrapped