1. Microscope
Basics

2. Where you View the Specimen!
Eyepiece-contains the ocular lens
Focuses ocular lens
Body Tube
Holds the high and low power objective lenses; can be rotated to change magnification
Use to support the microscope when carried
Nosepiece
Arm
Magnification ranges from 4X to 40X
Objective Lenses
Supports the slide being viewed
Stage
Hold the slide in place
Stage Clips
Moves the stage up and down for focusing
Coarse Adjustment
Regulates amount of Light on the specimen
Diaphragm
Moves the stage slightly to sharpen the image
Fine Adjustment
Projects light upwards through the diaphragm, the specimen, and the lenses
Light Source
Supports the microscope
Base

3. Microscope Lenses Eyepiece Medium (yellow) Power Objective Lens
Holds Ocular Lens
Magnification of 10X
Low Power (red) Objective Lens “Scanning”
Magnification of 4x
Used for Scanning
Use Coarse Focus Knob
Medium (yellow) Power Objective Lens
Magnification of 10X
Used for Scanning
Use Coarse Focus Knob
High Power Objective Lens
Magnification of 40X
Used for Fine Focusing
Seeing the details
Use Fine Focus Knob ONLY
Do NOT use Coarse Focus Knob

4. The Light Source sends light through the slide.
The Iris Diaphragm regulates the amount of light from the light source that passes through the slide.
The coarse adjustment should only be used in scanning and low power objectives. It is used for seeing the slide.
You should use the fine adjustment while in the high power objective. It is used for detailed focus.

5. The stage clips hold the slide and are located on the stage which is moved to view different areas of the slide and specimen.
All of the objectives are held on the nosepiece which rotates to change the power of magnification.
The scanning lens is usually 4x magnification and is used for initial scanning and specimen location on the slide.

6. The low or medium power objective lens is usually 10x magnification and is also used for scanning.
The high power lens is usually 40x or 100x and is used for detailed viewing of a specimen.

7. Fill Your Calculations AND Your answer on Your Worksheet!!!
What’s my power?
To calculate the power of magnification, MULTIPLY the POWER of the Ocular Lens by the POWER of the Objective
What is the power of magnification for the Ocular Lens (shown) and the 40x Objective Lens (shown)?
Fill Your Calculations AND Your answer on Your Worksheet!!!

8. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

9. Do’s and Don’ts of Microscope Labs!
USE TWO HANDS
Always carry microscopes with one hand on the ARM and the other UNDER the BASE!

10. Do’s and Don’ts
NEVER use Coarse Focus Knob with High Power Lens…it Breaks Slides!
“Ooops, I broke a slide”
Accidents Happen. Don’t FREAK OUT!
Don’t try to clean it up by yourself!
Raise your hand and a teacher will help you!

11. Cleaning UP! Always do 5 Things! Remove Your Slide Wrap Your Cord
Lower Your Stage
Point your lower power objective at the stage
Put Microscope Back in Designated Area Arm Out

12. You do not need to use the stage clips when viewing wet-mount slides!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
You do not need to use the stage clips when viewing wet-mount slides!
5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.