Requirement of DCC, but not neogenin, expression for the basal distribution of GFP–Myo X. (A) 3D confocal imaging analysis of NLT cells expressing GFP–Myo.

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Requirement of DCC, but not neogenin, expression for the basal distribution of GFP–Myo X. (A) 3D confocal imaging analysis of NLT cells expressing GFP–Myo X in the absence or presence of miRNA-DCC. Requirement of DCC, but not neogenin, expression for the basal distribution of GFP–Myo X. (A) 3D confocal imaging analysis of NLT cells expressing GFP–Myo X in the absence or presence of miRNA-DCC. The front (F), putative front (“F”), and stable (S) regions are marked. The two rows show projected views from the points of apical (top) and a Z-axis (side) of the same cell. The apical Myo X puncta are marked with white arrows, and the basal Myo X puncta is indicated by a green arrow. Scale bars: 20 μm. (B,C) Quantification of GFP–Myo X puncta distribution at the ‘apical’/non-basal region (B) and at the basal filopodia tip (C). The GFP puncta that are away from the basal side based on the Z-axis view were counted as ‘apical’ GFP puncta. Mean + s.d. from three different experiments is shown. (D) Comparison of the velocities of GFP–Myo X puncta in the presence or absence of miR-DCC or miR-neogenin. The velocities were analyzed as described in Figs 1, 2 and 3. 25 filopodia and 32 GFP–Myo X puncta from four GFP–Myo X-positive cells were analyzed. Mean + s.d. from three different experiments are shown; *P<0.01. Yu Liu et al. J Cell Sci 2012;125:751-762 © 2012.