1. High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis.
High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. (A) Mean fluorescence analyzed by flow cytometry, demonstrating binding of integrin β1 and β1-3-1 to different concentrations of soluble FITC-fibronectin. (B) Images of assembled fibronectin-biotin on GE11 cells expressing β1 or β1-3-1; fibronectin is red and nucleus blue. Scale bar: 50 μm. (C) Western blot analysis of RhoA activity assay on lysates of GE11 cells expressing indicated integrins. Quantification shows relative RhoA activation ± s.d. compared with GEβ1 cells from two independent experiments. (D) Images of GE11 cells expressing β1 integrin or β1-3-1 stained for paxillin (green) and F-actin (red). Scale bar: 10 μm.
Stephan Huveneers et al. J Cell Sci 2008;121:
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