Mild Therapeutic Hypothermia Protects the Brain from Ischemia/Reperfusion Injury through Upregulation of iASPP Liu Xiangrong 1, 2, 3 ;Wen Shaohong 1, 2, 3 ;Zhao Shunying 1 ;Yan Feng 2, 3 ;Zhao Shangfeng 4 ;Wu Di 1, 2 ;Ji Xunming 1, 2, 3, 5 ; 1 China-America Joint Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, China. ; 2 Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China. ; 3 Cerebrovascular Diseases Research Institute, Xuanwu Hospital of Capital Medical University, Beijing, China. ; 4 Department of Neurosurgery, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing, China. ; 5 Department of Neurosurgery, Xuanwu Hospital of Capital Medical University, Beijing, China. ; Figure 5. Effect of iASPPi and iASPPi plus hypothermia on the expression of iASPP and its targets in MCAO mice. A Representative protein in bands of iASPP, Puma, Bax and cleaved caspase-3 at 24 h after cerebral ischemia from the Western blot. Actin served as a loading control. B Densitometric quantification of iASPP, Puma, Bax and cleaved caspase-3 measured by Western blot. C-casp3, cleaved caspase-3, n = 4-6 per group. 37#cod#x000B0;C, normothermia. 33#cod#x000B0;C, mild therapeutic hypothermia. Small interfering RNA siRNA was intracerebroventricularly injected. Coni, control siRNA; iASPP siRNA iASPPi. Data are expressed as mean #cod#x000B1; SEM. N.S., not significant. #cod#x0002A; p #cod#x0003C; 0.05, #cod#x0002A;#cod#x0002A; p #cod#x0003C; 0.01, #cod#x0002A;#cod#x0002A;#cod#x0002A; p #cod#x0003C; 0.001, by one-way ANOVA and Tukey#cod#x02019;s test. null,null,0(0),null-null. Doi:10.14336/AD.2017.0703