1. Pkc#cod#x003B4; Activation is Involved in ROS-Mediated Mitochondrial Dysfunction and Apoptosis in Cardiomyocytes Exposed to Advanced Glycation End Products (Ages)
Yang Yao-Chih 1 ;Tsai Cheng-Yen 2, 3 ;Chen Chien-Lin 4 ;Kuo Chia-Hua 5, 6 ;Hou Chien-Wen 5 ;Cheng Shi-Yann 7, 8, 9 ;Aneja Ritu 10 ;Huang Chih-Yang 11 ;Kuo Wei-Wen 1 ;
1 Department of Biological Science and Technology, College of Biopharmaceutical and Food Sciences, China Medical University, Taiwan. 2 Department of Pediatrics, China Medical University Beigang Hospital, Taiwan. 3 School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taiwan. 4 Department of Life Sciences, National Chung Hsing University, Taiwan. 5 Laboratory of Exercise Biochemistry, University of Taipei, Taipei, Taiwan. 6 Graduate Institute of Physical Therapy and Rehabilitation Science, China Medical University, Taiwan. 7 Department of Medical Education and Research and Department of Obstetrics and Gynecology, China Medical University Beigang Hospital, Taiwan. 8 Department of Obstetrics and Gynecology, China Medical University An Nan Hospital, Taiwan. 9 Obstetrics and Gynecology, School of Medicine, China Medical University, Taichung, Taiwan. 10 Department of Biology, Georgia State University, Atlanta, GA 30303, USA. 11 Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan ; Graduate Institute of Chinese Medical Science, School of Chinese Medicine, China Medical University, Taichung, Taiwan ; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan. ;
Figure 2. Among PKC isoforms, AGE-BSA specifically induces PKC#cod#x003B4; protein expression and phosphorylation in cardiac cells. A Cells were treated with different doses of AGE-BSA with or without ROS scavengers NAC and mitochondrial complex I inhibitor Rote as indicated for 24 h. Western blot analyses were performed with antibodies against the PKC isoforms. These are cropped blots, full-length blots are presented in Suppl. Figure S2. B The densitometry measurements show the quantitative results of the western blots. Bars indicate the mean #cod#x000B1; SEM obtained from experiments performed in triplicate. #cod#x0002A; P #cod#x0003C;0.05 and #cod#x0002A;#cod#x0002A;#cod#x0002A; P #cod#x0003C;0.001 compared with the control group; ### P #cod#x0003C;0.001 compared with the AGE-BSA 300 #cod#x003BC;gml group.
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