1. CLARITY for High-resolution Imaging and Quantification of Vasculature in the Whole Mouse Brain
Zhang Lin-Yuan 1 ;Lin #cod#x0002A;Pan 2 ;Pan Jiaji 3 ;Ma Yuanyuan 1 ;Wei Zhenyu 4 ;Jiang Lu 3 ;Wang Liping 1 ;Song Yaying 1 ;Wang Yongting 3 ;Zhang Zhijun 3 ;Jin Kunlin 5 ;Wang Qian 2 ;Yang Guo-Yuan 1, 3 ;
1 Department of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China ; 2 Medical Image Computing Lab and ; 3 Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai , China ; 4 Shanghai Ninth People#cod#x02019;s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai , China ; 5 Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, TX76107, USA ;
Figure 6. Visualization and quantification of the vasculature in the penumbra of tMCAO and sham mouse brain. A Representative 3D image of the vasculature stained with claudin-5 in the penumbra of C57BL6J mouse brain from the control and tMCAO group after 24 hours of reperfusion. The imaging volume was 504 #cod#x003BC;m#cod#x000D7;504 #cod#x003BC;m#cod#x000D7;886 #cod#x003BC;m, with a voxel size of 0.99 #cod#x003BC;m#cod#x000D7;0.99 #cod#x003BC;m#cod#x000D7;2.00 #cod#x003BC;m. Scale bar=250 #cod#x003BC;m. B Mouse brain coronal section indicating core and penumbra region of the infarct area and the box indicating the area that was sampled. Quantification of the fractional vascular volume and the normalized vessel length in the controls and in the tMCAO group after 24 hours of reperfusion C and D. Data are mean #cod#x000B1; standard error, n=3 per group. #cod#x0002A; p #cod#x0003C; 0.05, tMCAO vs. control. tMCAO: transient middle cerebral artery occlusion.
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