Figure 1. Representative mismatched amplification mutation assay polymerase chain reaction amplicons for detection of 16u>c rrs mutation in 14 Mycobacterium.

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Figure 1. Representative mismatched amplification mutation assay polymerase chain reaction amplicons for detection of 16u>c rrs mutation in 14 Mycobacterium tuberculosis isolates. (A) The 233-bp wild type rrs allele amplicons obtained from rrs-16 wt and rrs-RM primers (arrow). (B) The 233 bp-16u>c rrs mutant allele amplicons obtained from rrs-16 mt and rrs-RM primers (arrow). Numbers on the left are DNA sizes (bp). Lane M: GeneRuler 100 bp DNA Ladder (Fermentas, Vilnius, Lithuania); lane 1: no template control; lane 2: rrs wild type allele control (H37Rv); lane 3: rrs-16u>c mutant allele control; lane 4–17: clinical M. tuberculosis isolates. Figure 1. Representative mismatched amplification mutation assay polymerase chain reaction amplicons for detection of 16u>c rrs mutation in 14 Mycobacterium tuberculosis isolates. (A) The 233-bp wild type rrs allele amplicons obtained from rrs-16 wt and rrs- RM primers (arrow). (B) The 233 bp-16u>c rrs mutant allele amplicons obtained from rrs-16 mt and… Tuberc Respir Dis. 2017 Apr;80(2):159-168. https://doi.org/10.4046/trd.2017.80.2.159