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Chapter 10
Author: Kelly Elkins
© 2013 Elsevier, Inc. All rights reserved.

2. © 2013 Elsevier, Inc. All rights reserved.
Figure PCR primer locations for TPOX primers used to amplify Homo sapiens thyroid peroxidase (TPO) on chromosome 2; NCBI Nucleotide Sequence (Accession M68651): repeat (gray), and sample designed primers (arrows and bold).
© 2013 Elsevier, Inc. All rights reserved.

3. © 2013 Elsevier, Inc. All rights reserved.
Figure Exponential amplification curves that show the results of forty cycles of gradient real-time PCR using primers depicted in Figure Curves of amplified K562 DNA (from 1 ng starting material) as detected by SYBR green I.
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4. © 2013 Elsevier, Inc. All rights reserved.
Figure A. Melting curve plot from °C showing the temperature inflection at 79.5 °C for the K562 DNA (left image). B. First derivative plot of the melting curve from °C showing the predominant peak at 79.5 °C for the K562 DNA (right image).
© 2013 Elsevier, Inc. All rights reserved.

5. © 2013 Elsevier, Inc. All rights reserved.
Figure Sample 2% agarose gel of PCR of K562 DNA using designed primers. Lanes 1 & 7 contain the DNA Logic Ladder (sizes indicated), Lane 2 contains a low molecular weight ladder ( bp) (sizes indicated) and Lanes 3-6 and 8-9 contain amplicons produced using experimental designed PCR primers. Lane 5 contains the amplicon produced from the primers shown in Figure 10.1.
© 2013 Elsevier, Inc. All rights reserved.

6. © 2013 Elsevier, Inc. All rights reserved.
Figure Setup of gradient PCR amplification cycle and plate temperature protocol on BioRad iQ5.
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7. © 2013 Elsevier, Inc. All rights reserved.
Figure Setup of gradient PCR run protocol on BioRad iQ5
© 2013 Elsevier, Inc. All rights reserved.