Mitosis Root Tip Lab Observing Cells Under the Microscope

Eye Piece Tube Arm Objective lens Stage Stage Clamps Coarse Focus Revolving Nosepiece Arm Objective lens Stage Stage Clamps Coarse Focus Get the pupils to guess the name of the parts of the microscope before I show them Fine Focus Light Base

Drawing your Cells Underneath your drawing, provide a bar that indicates the size of your drawing For example, if your cell is 110 μm wide and your drawing is 11.0 cm wide, every cm of your drawing represents 10 μm – so draw a 5.0 cm bar and label it 50 μm Explain again on the board!!

General Steps for Making Biological Drawings Determine the diameter of the field of view (pg. 95) Formulas to remember: Estimate the size of microscopic objects (pg. 95) Make biological drawings (pg. 96) – use your checklists for help

My Drawing Cells Checklist My name: ___________________ Set up the lowest power objective lens ☐ Record the magnification number of the lowest power lens ☐ Place a transparent ruler on the stage and focus on the markings ☐ Record the distance in mm of the field diameter ☐ Convert mm to μm (1000 μm = 1 mm) ☐ Calculate field of view for any higher power lens (remember the formula) ☐ Remove the ruler from the stage and replace it with the specimen you would like to observe ☐ Focus on the specimen using the lowest power lens ☐ Switch to a higher power objective lens until you can see the specimen clearly and in detail ☐ Calculate the field of view of your microscope using the higher power objective lens (units: μm and mm) ☐ Calculate the fit number (amount of times your specimen seems to fit across the diameter of your field of view) ☐ Find the actual size of your specimen: divide field of view diameter by fit number ☐ Draw what you see – using a lead pencil ☐ Specimens are drawn using unlined paper and clean cut lines ☐ Drawing is clear and at least half a page in size ☐ Drawing is to the left of the page ☐ Labels are to the right of the drawing ☐ Lines are drawn straight and parallel ☐ Cell boundaries are shown ☐ Drawing has a printed and underlined title appearing below the drawing ☐ The scale bar is accurate and positioned between the title and your drawing ☐ Example of a good drawing (yours should be bigger)

Peer Review Drawing Cells Checklist My name: _______________ I am reviewing: _______________ Peer Review Drawing Cells Checklist Magnification number of the lowest power lens is recorded ☐ The field diameter is recorded ☐ Field of view for higher power lens is calculated ☐ Fit number (amount of times the specimen seems to fit across the field of view diameter) is calculated ☐ Actual size of specimen is found accurately: divide field of view diameter by fit number ☐ Lead pencil was used to draw what the peer saw ☐ Specimens are drawn using unlined paper and clean cut lines ☐ Drawing is clear and at least half a page in size ☐ Drawing is to the left of the page ☐ Labels are to the right of the drawing ☐ Lines are drawn straight and parallel ☐ Cell boundaries are shown ☐ Drawing has a printed and underlined title appearing below the drawing ☐ The scale bar is accurate and positioned between the title and drawing ☐ Example of a good drawing (yours should be bigger)