Prerequisites (C or better): BSC1010, CHM1046, PCB3063 Cell Biology PCB 4023 Tu/Th-12:30-1:45 pm CP 151 Prerequisites (C or better): BSC1010, CHM1046, PCB3063 Capacity Class is FULL PCB 4023 Lab ?

Course Description ---This is a required course to provide undergraduate students majoring in Biology (Marine Biology or others) with an in depth looks at the morphology and function of structures at the cellular level.

Course Description ---Topics include Structure and function of cell membrane systems, Intracellular transport, Cellular communication, motility, and division, HIV retro-virus, Insulin function and action Cancer biology.

DO something ! Learning Goals --To gain a solid knowledge base of the structure and function of cells. --To become familiar with the appropriate vocabulary. with the methods used by scientists to study cell biology. with primary literature in cell biology. DO something !

M. Alejandro Barbieri Office/Lab: AHC-1: 318C / AHC-1:214 Phone: 305-348-7536----lab 7535---office E-mail: barbieri@fiu.edu (This is the better way to contact me) Office hours: -Tu: 2:00 to 4:00 pm -Th: 3:00 to 5:00 pm -By appointment ONLY.

Course Materials, Text: Required: Cell and Molecular Biology, Concepts and Experiments, 7th/ 8th Ed. by G. Karp Recommended Principle of cell biology By Plopper (2014) ISBN 978-1284047608 Biochemistry: A Short Course By John L. Tymoczko (2009) ISBN 0716758407 Principles of Cancer Biology By L. J. Kelinsmith (2006) ISBN 0-8053-4003-3 Medical Cell Biology By S. R. Goodman ( 2008) ISBN 97801237458 Additional readings will be posted on the website or in the class. Although these will be announced in class, students are responsible for checking the class web page regularly for announcements and appendices.

Textbooks www.abebooks.com

Attendance Attendance will not be taken but is encouraged. Students are expected to attend class regularly, to arrive on time, and to remain in class until the end of the class period. Class attendance will help you master the course material. Furthermore, exam questions may cover material not found in the text book. If you miss class, you will still be responsible for any material disseminated or work that is assigned or due in your absence.

Grading Policy A 90-100 points B 80-89 points C 70-79 points D F <60 points

Grading Policy Exams: --Four (4) --NO Final exam --Each exam has a maximum of 20 points …….80 points --Total points:69 --ONE Class (Special) Assignment (10 points) --Four (4) Quizzes --Each quizz has a maximum of 2.5 points …….10 points For example Exam1: 17 pts Exam2: 20 pts Exam3: 17 pts Exam4: 15 pts

Grade Curving =70% Mean

Grade Curving Exam 1 Max: 31 pts Mean= 64.5% 70% represents the mean is 21.7pts 20 pts=Mean of the class is 64.5% =21.7 pts 20 pts

Grade Curving Max: 31 pts 70% represents the mean is 21.7pts Exam 2 30 pts=Mean of the class is 96.7% Exam 2 Mean=96.7% Pts=30+0 =21.7 pts 30 pts

Grade Curving Exam3 Exam3 Mean= 58.06% Max: 31 pts Pts=18+3.7.=21.7 70% represents the mean is 21.7 pts 18 pts=Mean of the class is 58.06% Exam4 Max: 31 pts 70% represents the mean is 21.7pts 15 pts=Mean of the class is 48.38% Exam4 Mean= 48.38% Pts=15+6.7=21.7

Exam 2 Mean= (96.7%)=30 pts Exam 1 Mean=(64.5%) 20 pts Student 1= 22 pts + 0= 22 pts Student 2= 29 pts + 0= 29 pts … Student 150=24 pts +0=24 pts Student 1= 20 pts + 1.7 pts = 21.7 pts Student 2= 28 pts + 1.7 pts = 29.7 pts … Student 150=25 pts +1.7= 26.7 pts Exam =3 Mean=(58.06%)= 18 pts Exam =4 Mean=(48.38%)= 15 pts Student 1= 15 pts + 3.7 pts= 18.7 pts Student 2= 20 pt + 3.7 pts= 23.7 pts … Student 150=22 pts + 3.7 pts= 2.7 pts Student 1= 12 pts + 6.7 pts= 18.7 pts Student 2= 20 pt + 6.7 pts= 26.7 pts … Student 150=22 pts + 6.7 pts= 28.7 pts

Final grade Exam 1 + Exam 2 + Exam 3+ Exam 4 + A + B Quizzes Special Assignment

Exams /Quizzes 1-Four quizzes /exams Quiz 1-Ch1, Ch2, Ch3; 1/31/17 Exam 1- Ch1, Ch2, Ch3; 2/2/17 Quiz 2-Ch4, Ch7, Ch9 – 2/28/17 Exam 2- Ch4, Ch7, Ch9 – 3/2/17 Quiz 3-Ch8, Ch15 – 3/28/17 Exam 3- Ch8, Ch15 – 3/30/17 Quiz 4-Ch12, Ch14, Ch16, Ch17, Ch18 – 4/18/17 Exam 4-Ch12, Ch14, Ch16, Ch17, Ch18 – 4/20/17 Special Assignment -4/6/17 2-Material covered in lecture/ book/ appendices

Exams THINK about it Recognize it 3-Exam questions - any combination of multiple choice, true/false and fill in the blank. 4-Exams will be closed book / note / computer / cell phone / etc . 5-You will be asked to show your Panther ID card on exam day so be sure to bring it to class. 6-Two type of questions: Conceptual and Specific THINK about it Recognize it

Special Assignment Instructions will be sent via email on 1/17/17

Look for……Teaching… Cell Biology http://www.fiu.edu/~barbieri Look for……Teaching… Cell Biology General course information including the course syllabus as well as class announcements, chapters and additional required readings. It is your responsibility to check the course website regularly for updated information and announcements

F0 and IN grades A student who earns a failing grade based on the total number of points and fails to complete at least 60% of the course requirements will receive a grade of F0 (F zero). A grade of IN (Incomplete) will only be given in extremely rare circumstances where unusual circumstances occur after the Drop date. For a student to receive an IN, the student must have completed at least half the course work and have a passing grade at the time of the incident. Professional documentation, including an address and telephone number must demonstrate that the student cannot complete the course due to factors beyond their control (severe illness, accident, death in the immediate family, etc.). The student must complete the course within two terms, otherwise the grade will automatically default to an F.

Academic Integrity Collaboration is an important aspect of scientific discovery as well as learning. While it is encouraged that students study together and share data with partners as appropriate, all assignments should be written independently by each student. When appropriate, sources (including the internet and personal communication) should be cited. Cheating and plagiarism are serious offenses and are NOT acceptable. If found to have cheated, or have enabled someone else to do so, the student will be subjected to disciplinary action that will include receiving a zero that cannot be dropped for the exam. A second occurrence will result in the student receiving an F for the course. If you are aware of such academic dishonesty, report it to the professor.

Behavioral Misconduct We (students and instructors) are responsible for maintaining an appropriate, orderly, learning environment in accordance with the mission of the University. Disruptive behavior will not be tolerated. All cell phones must be turned off. During exams all electronic devices must be turned off and put away. Students in attendance should listen and participate appropriately. Students who fail to adhere to the behavioral expectations outlined by the instructor and presented in the FIU Student Handbook may be subjected to discipline in accordance with the procedures described in the FIU Student Handbook. The student handbook also describes the University's policy on sexual harassment.

ADA Accommodation Statement If the student has a documented disability as described by the Rehabilitation Act of 1973 (PL 933-112 Section 504) and Americans with Disabilities Act (ADA) and would like to request academic and/or physical accommodations, please contact the Disability Resource Center (tel. 305-348-3532, TTY/TDD 305-348-3852), as soon as possible. Course requirements will not be waived, but reasonable accommodations be provided as appropriate. It is then the student's responsibility to contact and meet with the instructor as early as possible.

Cell Biology The study of the function, structure and activities of cells--Cellular Level-- Exciting time as disciplines are cooperating to study cells (biologists, chemists, physicists, computer scientists, mathematicians)

Soft drinks composition vs. Cell Biology Source of Energy?

SUGARS (high-fructose corn syrup) Na+/K+ Phosphoric acid pH~2.1 Caffeine H20 = CO2 gas-----CO3H- liquid Nature’s herbs extract

~ 44 grams (fructose, glucose and sucrose) ~ 4 to 6 grams/150 lb (68 kg) (glucose)

Intestinal Lumen Blood (?) SUGARS Intestinal Lumen Blood (?)

Pancreas Blood beta cells (sugars) (?)

Glucose Regulation of β-pancreatic Cells ? (Blood) Sugars Blood

SECRETORY-EXOCYTIC PATHWAY

Insulin Receptor-Liver- Blood

SECRETORY-EXOCYTIC PATHWAY ENDOCYTIC PATHWAY

Nerves Connect at Synapses

Life cycle of a synaptic vesicle Figure 12-10

http://stke. sciencemag. org/content/sigtrans/suppl/2004/12/20/2004 http://stke.sciencemag.org/content/sigtrans/suppl/2004/12/20/2004.264.re19.DC2/slowtrack2.swf

SECRETORY-EXOCYTIC PATHWAY RECYCLING ENDOCYTIC PATHWAY

Phagocytosis of Listeria moncytogenes in macrophages expressing Rab5-GFP

Three Americans won the 2013 Nobel Prize in Physiology or Medicine for discovering the machinery that regulates how cells transport major molecules in a cargo system that delivers them to the right place at the right time.

Cholesterol and Cell Biology (good, bad, ugly) 186 mg of cholesterol/egg ~35 grams (~10 grams/blood) of cholesterol/150 lb (68 kg) ~1 to 1.5 gram is made by the Liver /day ~25 to 30 % is in your Brain

Which one is ….. good, bad and ugly Cholesterol? Oxidized form Reduced form LDL HDL

CHAPTER 1 “Cell” --The basic structural and functional unit of living organisms --The smallest structure capable of performing the essential functions characteristic of life --Others

chemistry of living systems Modern Cell (and Molecular) Biology Cytology the study of cell structure Genetics the study of the behavior of genes Biochemistry the study of the chemistry of living systems

The study of cells 1830’s microscopes had higher magnification and improved resolution (1 micrometer= m) magnification – the ability to make images of objects larger resolution – minimum distance that can separate two points that still remain identifiable as separate points when viewed through a microscope

The World of the Micrometer a.k.a. micron, µm 10-6 meter

The World of the Nanometer nm = 10-9 meter Angstrom (Å) = 0.1 nm

The study of cells 1839 – Schwann’s cell theory All organisms consist of one or more cells The cell is the basic unit of structure for all organisms 1855 –third component added by Rudolf Virchow All cells arise only from pre-existing cells (omnis cellula e cellula)

Cell and Molecular Biology * * * * * * * (*) Appendix