DNA .
Griffith exp. Hershey and Chase exp.
DNA Deoxyribonucleic acid: DNA two stranded double helix Purines: adenine and guanine Pyrimidines: thymine and cytosine Bonding: A-T (double bond) and C-G (triple bond) Nucleotide= base-sugar-phosphate 5’ 3’ DNA sequence is read in a 5’ to 3’ 3’ 5’
DNA Replication: unzipping DNA double helix Replication: the process by which DNA is copied during the cell cycle (s stage) Enzymes and other proteins carry out the work of replication Once the strands are separated: free nucleotides within the nucleus can then be paired up with the separated strand in correct sequence DNA Polymerase: enzyme that bonds the new nucleotides together Also proofreads and corrects any base pairing errors
Replication Helicase: enzyme that unzips the DNA double helix into a Y shaped replication fork Leading strand and lagging strand: each strand becomes a template for a new complimentary strand Semiconservative replication: New DNA consists of one old template strand and one new complimentary strand
Replication Primase: starts the complimentary strand with short segments called RNA primers DNA polymerase bonds to the primers. This begins elongation: DNA nucleotides added to the complimentary strand Leading strand assembles continuously 5’ to 3’ Lagging strand assembles in short pieces called Okazaki fragments DNA Ligase: joins the fragments together
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