Synthesis of Sperm &Seminal Plasma Sperm Production Maturation &Storage Seminal Plasma Semen Testes: Sertoli Cells Epididymis & Vas Deference Epididymis & Vas Deference Seminal Vesical 2/3 & Prostate 1/3 Seminal Vesical 2/3 & Prostate 1/3 Sperm & Seminal Plasma Semen Days

Male genital anatomy

sperm

Sperm morphology HeadCarries genetic material, has elements for penetration oocyte DimensionSize LengthWidth 4-6 u2.5 – 3.5 u ShapeOval Acrosome40 – 65% area of head NeckContain mitochondria, provides energy DimensionSize LengthWidth uMaxi. 1.0 u Shap e Oval TailGive cell a straight line movement DimensionSize Length 45 u

Oocyt

Sperms+Oocyt

Zygote

Embryo

Blastocyst

Hatching Blastocyst

Implantation of Embryo

Semen analysis includes the examination of : Spermatozoa Other cells present in semen Seminal fluid Sperm function test

Basic Information Abstinence period( 3-5 days) Collection method Time of collection (<30 min. in lab.) Place of collection

Semen Fluid Analysis Macroscopic examination Microscopic examination pH Volume appearance (abnormal smell, colour or viscosity) liquefaction time (above 60 minutes Sperm concentration Sperm motility Sperm vitality Sperm morphology Cells (WBC,Epithelial,..

Normozoospermia When all the spermatozoal parameters are normal together with normal seminal plasma,WBCs and there is no agglutination. OligozoospermiaWhen sperm concentration is < 20 million/ml. Asthenozoospermia Fewer than 50% spermatozoa with forward progression(categories (a) and (b) or fewer than 25% spermatozoa with category (a) movement. TeratozoospermiaFewer than 50% spermatozoa with normal morphology. Oligoasthenoteratozoosp ermia Signifies disturbance of all the three variables (combination of only two prefixes may also be used). AzoospermiaNo spermatozoa in the ejaculate. AspermiaNo ejaculate. Definitions of semen classifications

Andrology Lab. set up Instruments Sperm meter Microscope Plastic ware &Disposable TubesPasture pipette Container

Sperm Fructose Sperm Morph Sperm VitalitySperm HOS Sperm pH Sperm Function Tests

Sperm concentration &motility

Sperm concentration= No. of Sperm in 10 square x depth x dillution x 10 4 /ml area counted = No. of Sperm x 10 x 1 x 10 4 /ml 0.1 =No. of Sperm x 10 6 /ml

Sperm Morphology - processing

Sperm Vitality Test

HOS TEST

Normal pH : 7.6 – 8.6 Abnormal pH : 9.4 Sperm PH

Results: Present /Absent Fructose Test (qualitative)

AgePatient Name IVF NO Consultant Sample ID / /2011Date DAbstinenceLABLocationWhiteColorTime Collected AgglutinationPH MLVolumeTime In Lab ViscosityCollection MethodPortion Lost MinLiquefaction Time (WHO)Result Sperm Characteristics >=20 Million/MLSperm Concentration >=40 Million/EjaculateTotal Sperm Count >=50%*Total Motility. >=25% -Grade. A ( Rapid progressive movement ) >=25% -Grade. B ( Slow progressive movement) 25% -Grade. C ( Non progressive movement ) >=75% Life Sperm-% Vitality Test Macroscopic examination Microscopic examination Al-Boshra Specialized Center For A ssisted Reproduction & IVF SFA REPORT

Normal Forms % ResultNormal range Abnormal Head % WBCs1-5/HPF Abnormal MidPiece % RBCs1-5/HPF Cytoplasmic Droplet % Epithelial Cell Abnormal Tail % Round Cells Comment:- MORPHOLOGY ANALYSIS DATA:- Semenologist BIO.: Sign:_______________________

Comment:- NORMOZOOSPERMIA ?? Comment:- Oligozoospermia ?? Comment:- Asthenozoospermia?? Comment:- Teratozoospermia

Comment:- Oligoasthenoteratozoospermia ?? Comment:- Azoospermia what we do?

IVF LAB. Dishes CO2 Incubator ICSI Zygote EmbryoBlastocyst 25/10/2010

Twince at 6/1/2011 Twince

Thank you