1. Andrology and IVF Laboratory Services
Charles L. Bormann, PhD
IVF/Andrology Laboratory Supervisor 1

2. Laboratory Regulations
Mark P.D. Dow PhD, HCLD (Laboratory Director)
Charles L. Bormann MS, PhD (Laboratory Supervisor)
Lynn Boehnlein BS, MT,TS (Senior Embryologist)
Elizabeth Schleinz BS, MT,TS
(Senior Embryologist)
Clinical Laboratory Improvement Amendments (CLIA)
Food and Drug Administration (FDA)
College of American Pathologists (CAP)
Society for Assisted Reproductive Technology (SART)

3. Semen Analysis Abstain 2-3 days prior to analysis
Collect in an approved sterile cup or condom
Do not use lubricants for collection
Sample processed within 1 hour of collection

4. World Health Organization
Parameters
Volume
>2ml pH
Sperm Concentration
>20 million/ml
Sperm Motility
>50%
Progression/Speed ≤3
Sperm Viability
≥50%
Morphology (Krueger)
≥14%
Round Cells
≤5/hpf
DNA Fragmentation
≤30%
Viscosity

5. Sperm Motility
*Motility
*Forward Progression
*Velocity

6. Sperm Morphology

7. Sperm DNA Fragmentation

8. Sperm DNA Fragmentation
Density Gradient
Density Gradient
Spin Wash
Swim-Up

9. Sperm Viability Tests Viability Stain Pentoxifylline
Hypo-Osmotic Swelling (HOS)

10. Donor Sperm
Purpose: Offer a chance at pregnancy to those without a male partner, or if male partner’s sperm are absent, unable to fertilize, carry a genetic disease, etc

11. Donor Sperm
Photos and personal information also available

12. Inter-Uterine Insemination (IUI)
Semen
Wash
DGS
Remove seminal plasma
Remove debris (WBC, dead sperm)
Enrich highly motile sperm population
Centrifuge,
remove supernatant
Centrifuge,
remove top layers
Wash
Wash
Count/Assess
Reconstitute
Load catheter

13. Intracytoplasmic Sperm Injection (ICSI)

14. Fertilization ~ 18h post insemination Female Pronucleus Male2 1 1 3 1 2 4 6 5 3

15. Embryo Development

16. The in vitro environment was designed to mimic mother nature
In Vitro Culture
The in vitro environment was designed to mimic mother nature

17. Embryo Development Days 1-3 Days 4-6 2-Cell 4-Cell 8-Cell Morula
Blastocyst
Hatching Blastocyst
Days 4-6

18. Day 3 vs. Day 5 Embryo Transfer
8AA
2CB
3BB
4AB
4AA

19. Preimplantation Genetic Diagnosis
Recurrent Miscarriage
Unsuccessful IVF Cycles
Unexplained Infertility
Advanced Maternal Age
Male Factor Infertility
Inherited Genetic Disorders

20. Preimplantation Genetic Diagnosis (PGD)
Embryo Culture Days 1-3
Day 3 Embryos (8-Cell)
Blastomere Biopsy Day 3
Day 5-6 Blastocyst Culture, Embryo Transfer and Possible Freeze

21. Assisted Hatching Application Abnormally thick zona pellucida
Frozen/thawed embryos/oocytes
Prior failed IVF
>37 yrs of age
Elevated FSH

22. Fertility Preservation

23. Sperm Freezing Frozen Indefinitely Simple to Freeze
No documented risks from using frozen sperm

24. Egg Freezing -Considered Experimental
-Option for the preservation of fertility in women with malignant diseases
-Not ideal for women wishing to delay childbirth

25. Embryo Freezing -Preserve additional embryos for future cycles
-Embryos frozen indefinitely

26. Fresh Embryos From Non-Donor Oocytes
2007 SART National Summary:
Age
Fresh Embryos From Non-Donor Oocytes
<35
35-37
38-40
41-42
Number of cycles
38,372
21,707
19,099
8,865
Percentage of cycles resulting in pregnancies
45.8
37.2
28.2
18.5
Percentage of cycles resulting in live births
39.9
30.5
21.0
11.7
~5-10% Lower with Frozen Embryos

27. Semen Analysis Identification
Labeling
Chain of Custody
Isolation
Verification
Name: John Doe
DOB: March, 23, 2010
MR#:

28. IVF/ICSI Identification
Labeling
Chain of Custody
Isolation
Verification

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