Animal Health Division Brenda Keavey Aquatic Health Certifications

West Virginia Department of Agriculture’s Laboratory in Charleston History first fish bacterial cultures performed ’s WV Dept. of Education samples Few requests from producers (approximately 2 requests annually)

West Virginia Department of Agriculture’s Laboratory in Charleston Progression of Laboratory Testing for Fish 2006 Sampling training at Micro Technologies Inc Diagnostic testing training at Micro Technologies Inc. Sampling procedures training Testing areas included bacterial, viral, molecular and parasitic

West Virginia Department of Agriculture’s Laboratory in Charleston Progression of Laboratory Testing for Fish 2008 Obtained Viral Hemorrhagic Septicemia Virus (VHSV) permit for positive cultures. Prepared for DNR testing – USDA grant for surveillance of VHSV. Performed viral cell culture transfers and testing samples for approximately 1 year Suspended cell culture testing due to lack of samples and direction of lab’s role in aquaculture Cell culture testing reinstated at the end of the year due to the formation of the Aquaculture Board.

West Virginia Department of Agriculture’s Laboratory in Charleston Current Laboratory Testing Goals for Bacterial Cultures Bacterial cultures for a number of organisms Aeromonas species Streptococcus species Carnobacterium piscicola Vibrio species Edwardsiella species Yersinia ruckeri Flavobacterium species

West Virginia Department of Agriculture’s Laboratory in Charleston Current Laboratory Testing Goals for Training Rob Nottingham, Laboratory Supervisor Animal Health Division Microbiologist Regulatory and Environmental Affairs Division

West Virginia Department of Agriculture’s Laboratory in Charleston Current Laboratory Testing Goals for Certifications Become certified by USDA to perform cell culture for Cytopathic effect in FHM cell line 4 days after inoculation Courtesy of AFS Bluebook Viral Hemorrhagic Septicemia Virus (VHSV) Infectious Hematopoeitic Necrosis Virus (IHNV)

West Virginia Department of Agriculture’s Laboratory in Charleston Laboratory Testing Goals for 2011 – 2012 Bacterial Diseases of Fish Cultural Techniques Fluorescent Antibody Test (FAT) Polymerase Chain Reaction (PCR) Enzymed-Linked Immunosorbent Assay (ELISA) Courtesy of 2010 edition of the American Fisheries Society, Fish Health Section Blue Book Renibacterium salmoninarum (Bacterial Kidney Disease)

West Virginia Department of Agriculture’s Laboratory in Charleston Laboratory Testing Goals for 2011 – 2012 Viral Diseases of Fish Infectious Pancreatic Necrosis Virus (IPN) Birnavirus: The Queen's University of Belfast, kindly provided by Prof Stewart McNulty Cell Culture Fluorescent Antibody Test (FAT) Polymerase Chain Reaction (PCR)

Laboratory Testing Goals for 2011 – 2012 Parasitic Diseases of Fish Presumptive diagnosis - staining confirmation histopathology West Virginia Department of Agriculture’s Laboratory in Charleston Tetracapsuloides bryosalmonae Proliferative Kidney Disease (PKD) Courtesy of 2010 edition of the American Fisheries Society, Fish Health Section Blue Book

Laboratory Testing Goals for 2011 – 2012 Parasitic Diseases of Fish Ceratomyxa shasta Myxobolus cerebralis (Whirling Disease) West Virginia Department of Agriculture’s Laboratory in Charleston

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