In silico feedback for in vivo regulation of a gene expression circuit Shared by lxshen

Biological feedback Signals Biological control circuits Implement of control

Comparison of control circuits Synthetic biological circuits Nature circuits robust Difficulty in artificial circuits Not easy to control slow Electronic circuits Prosperous Easy to control More functions Fast

Interface

model Fourth-order linear ordinary differential equation Identification experiment to determine five parameters fluorescence delay (fluorescence ≠ gene expression)

Applying model Ⅰ Task: increasing fluorescence 4 or 7 times over a 7-h period Open-loop(OL)control Fail to regulate fluorescence to desired levels due to inaccuracies of the model and something unpredictable

Applying model Ⅱ Closed-loop(CL) feedback control Kalman filter

Significance More predictable and accurate Avoid the disadvantage of bio-circuits Facilitate synthetic systems

Q ﹠ A