AP Biology Chapter 17. RNA Processing
AP Biology Transcription -- another look The process of transcription includes many points of control when to start reading DNA where to start reading DNA where to stop reading DNA editing the mRNA protecting mRNA as it travels through cell
AP Biology Primary transcript Processing mRNA protecting RNA from RNase in cytoplasm add 5’ cap add polyA tail remove introns AUGUGA
AP Biology Protecting RNA 5’ cap added G trinucleoside (G-P-P-P) protects mRNA from RNase (hydrolytic enzymes) 3’ poly-A tail added A’s protects mRNA from RNase (hydrolytic enzymes) helps export of RNA from nucleus UTR
AP Biology Dicing & splicing mRNA Pre-mRNA mRNA edit out introns intervening sequences splice together exons expressed sequences In higher eukaryotes 90% or more of gene can be intron no one knows why…yet there’s a Nobel prize waiting… “ AVERAGE ”… “ gene ” = 8000b pre-mRNA = 8000b mature mRNA = 1200b protein = 400aa lotsa “ JUNK ” !
AP Biology Discovery of Split genes 1977 | 1993 Richard RobertsPhilip Sharp NE BioLabsMIT adenovirus common cold Discovered that the sequence of DNA Nucleotides that code for a polypeptide is usually split into segments (introns & exons)
AP Biology snRNPs small nuclear RNA RNA + proteins Spliceosome several snRNPs recognize splice site sequence cut & paste RNA as ribozyme some mRNA can splice itself RNA as enzyme Splicing enzymes
AP Biology Ribozyme RNA as enzyme Sidney AltmanThomas Cech 1982 | 1989 YaleU of Colorado
AP Biology Splicing details No room for mistakes! (Mistakes – Mutation) editing & splicing must be exactly accurate a single base added or lost throws off the reading frame AUG|CGG|UCC|GAU|AAG|GGC|CAU AUGCGGCTATGGGUCCGAUAAGGGCCAU AUGCGGUCCGAUAAGGGCCAU AUG|CGG|GUC|CGA|UAA|GGG|CCA|U AUGCGGCTATGGGUCCGAUAAGGGCCAU AUGCGGGUCCGAUAAGGGCCAU Met|Arg|Ser|Asp|Lys|Gly|His Met|Arg|Val|Arg|STOP|
AP Biology bases on mRNA are called Codons. Each codon specifies and amino acid. AUG is a start codon 3 stop codons Code is redundant several codons for each amino acid *What’s the value in redundancy in the genetic code? Universal code
AP Biology Alternative splicing Alternative mRNAs produced from same gene when is an intron not an intron… different segments treated as exons Hard to define a gene!
AP Biology Value of Alternative splicing? Allows a small number of genes to make many different proteins
AP Biology Domains (Gene can include many exons!) Modular architecture of many proteins separate functional & structural regions coded by different exons in same “gene”
AP Biology AAAAAAAAGTP 20-30b 3' promoter transcription stop transcription start introns The Transcriptional unit (a gene +) transcriptional unit TACACT DNA TATA 5' RNA polymerase pre-mRNA 5'3' translation start translation stop mature mRNA 5'3' UTR exons enhancer b
AP Biology cDNA (Complementary DNA) hill.com/olc/dl/120078/bio_h.swf hill.com/olc/dl/120078/bio_h.swf
AP Biology Any Questions??
AP Biology b 3' introns The Transcriptional unit transcriptional unit TACACT DNATATA 5' RNA polymerase 5'3' 5'3' exons enhancer b