1. AP Biology Discussion Notes Friday 02/06/2015

2. Goals for Today Be able to describe RNA processing and why it is EVOLUTIONARILY important. In a more specific way be able to describe what is happening in transcription of Prokaryotes vs. Eukaryotes Be good AP test takers!

3. Questions of the Day 2/6 How many chromosomes in Human Diploid cells? How does this compare to other organisms? Higher/Lower/Similar? Are all organisms Diploid Organisms?

4. RNA Processing EUkaryotes only! Not in Prokaryotes

5. 17.3: Eukaryotic cells modify RNA after transcription Enzymes in the eukaryotic nucleus modify pre-mRNA (RNA processing) before the genetic messages are dispatched to the cytoplasm During RNA processing, both ends of the primary transcript are usually altered Also, usually some interior parts of the molecule are cut out, and the other parts spliced together

6. Alteration of mRNA Ends Each end of a pre-mRNA molecule is modified in a particular way –The 5 end receives a modified nucleotide 5 cap –The 3 end gets a poly-A tail These modifications share several functions –They seem to facilitate the export of mRNA to the cytoplasm –They protect mRNA from hydrolytic enzymes –They help ribosomes attach to the 5 end

7. Figure 17.10 Protein-coding segment Polyadenylation signal 5 3 3 5 5 Cap UTR Start codon G P P P Stop codon UTR AAUAAA Poly-A tail AAA …

8. Split Genes and RNA Splicing Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions These noncoding regions are called intervening sequences, or introns The other regions are called exons because they are eventually expressed, usually translated into amino acid sequences

9. Figure 17.11 5 Exon IntronExon 5 Cap Pre-mRNA Codon numbers 1  30 31  104 Intron Exon 3 105  146 Poly-A tail

10. Split Genes and RNA Splicing RNA splicing removes introns and joins exons, creating an mRNA molecule with a continuous coding sequence

11. Figure 17.11 5 Exon IntronExon 5 Cap Pre-mRNA Codon numbers 1  30 31  104 mRNA 5 Cap 5 Intron Exon 3 UTR Introns cut out and exons spliced together 3 105  146 Poly-A tail Coding segment Poly-A tail UTR 1  146

12. In some cases, RNA splicing is carried out by spliceosomes Spliceosomes consist of a variety of proteins and several small nuclear ribonucleoproteins (snRNPs) that recognize the splice sites

13. Figure 17.12-1 RNA transcript (pre-mRNA) 5 Exon 1 Protein snRNA snRNPs Intron Exon 2 Other proteins

14. Figure 17.12-2 RNA transcript (pre-mRNA) 5 Exon 1 Protein snRNA snRNPs Intron Exon 2 Other proteins Spliceosome 5

15. Figure 17.12-3 RNA transcript (pre-mRNA) 5 Exon 1 Protein snRNA snRNPs Intron Exon 2 Other proteins Spliceosome 5 Spliceosome components Cut-out intron mRNA 5 Exon 1 Exon 2

16. Ribozymes Ribozymes are catalytic RNA molecules that function as enzymes and can splice RNA The discovery of ribozymes rendered obsolete the belief that all biological catalysts were proteins

17. Three properties of RNA enable it to function as an enzyme –It can form a three-dimensional structure because of its ability to base-pair with itself –Some bases in RNA contain functional groups that may participate in catalysis –RNA may hydrogen-bond with other nucleic acid molecules

18. The Functional and Evolutionary Importance of Introns Some introns contain sequences that may regulate gene expression Some genes can encode more than one kind of polypeptide, depending on which segments are treated as exons during splicing (alternative RNA splicing) Consequently, the number of different proteins an organism can produce is much greater than its number of genes

19. How does Chromosome number relate to complexity? Is there a relationship?

20. Question of the Day 2/6 How many chromosomes in Human Diploid cells? How does this compare to other organisms? Higher/Lower/Similar? Are all organisms Diploid Organisms?

21. Proteins often have a modular architecture consisting of discrete regions called domains In many cases, different exons code for the different domains in a protein Exon shuffling may result in the evolution of new proteins

22. Gene DNA Exon 1 Exon 2Exon 3 Intron Transcription RNA processing Translation Domain 3 Domain 2 Domain 1 Polypeptide Figure 17.13

24. RNA Processing Introns “in-between” non-coding region Exons “Expressed” -“exit” – coding region, exits the nucleus & move to the cytoplasm to find ribosomes

25. Where does RNA Processing fit in? Steps of Protein Synthesis 1. 2.

26. Questions?

27. Work Order Today 1.Turn in Reading Guide (331-336) 2.Work on AP Practice Questions –Try them on your own FIRST –Then use notes/book if you need to –Then discuss with someone else –Then score yourself 3.Work on Extra Credit 4.Read Double Helix ASK QUESTIONS!!!