1. Panniculus Dr. Preethi chekuri MD DVL

2. Introduction Occurs almost universally over the body surface, between the skin and deep fascia Absent from eyelids and male genitalia It varies in thickness with the race, age, sex, endocrine and nutrtional status In a normal person, fat constitutes 10% of body weight

3. Functions Acts as an insulating layer Protective cushion Thermogenesis Store of readily available energy Major endocrine organ Provides support and has cosmetic function (contours of face)

4. Embryology Pre – adipocyte appears in the mesenchyme at 14 th week of fetal life Pre – adipocyte terminally differentiate into either brown adipocyte or white adipocyte Development of brown fat begins at the 20 th week of gestation, reaches maximum at birth

5. Brown fat It is multilocular and metabolically very active with many mitochondria Surrounded by a greater capillary network than white fat ( partly responsible for the brown colour), such that heat can be rapidly tranferred into the circulation Most prominent in neck and upper thorax of fetus Adults have supraclavicular areas of brown fat

6. Histology of adipocytes Have a diameter upto 100 microns Mature fat cell has a characteristic signet ring appearance, because the oval nucleus is displaced to a side by a single, large, intracellular, fat containing vacuole, which is surrounded by perilipin

7. Arranged in lobules, which are separated by interlobular septa Each lobule consists adipocytes, stromavascular tissue being macrophages, fibroblasts, mast cells, pericytes, endothelial cells and pre-adipocytes

8. Blood, nerve and lymph supply BLOOD - Fat tissue has an abundant blood supply, each lobule being supplied by a terminal arteriole running along the septa Then breaks up to form capillaries LYMPH - Also contains a rich lymphatic plexus These lymph vessels penetrate deep fascia and drain into regional lymph nodes NERVE - Innervated by noradrenergic fibres of sympathetic nervous system and para sympathetic fibres

9. Adipogenesis It refers to the recruitment from multipotent stem cells in the mesenchyme and stromavascular tissue, and proliferation of pre adipocytes followed by their differentiation into mature fat cells -Glucocorticoids - growth hormone and -insulin, stimulate cells to terminally differentiate, but after a certain size, growth occurs through hyperplasia rather than hypertrophy

10. Energy homeostasis Catabolism and anabolism of fat depends on -nourishment, endocrine and nervous activity Insulin inhibits hydrolysis and breakdown of triglycerides, conserving the energy store Adipose tissue in vitro has a metabolic rate similar to a kidney and half that of liver

11. Outline of lipogeneis in an adipocyte

13. Fat as an organ Apart from the subcutaneous fat, 20% of fat tissue occurs internally, in mediastinal and retroperitoneal tissues, mesentry, marrow and other individual organs including blood vessels In view of the recent obesity epidemic, increase in the upper body and visceral fat are associated with an increase cardiovascular and metabolic risks

14. Fat as an endocrine organ

15. Adiponectin Protein with structural similarity to collagen and C1q Has autocrine/paracrine effects locally and endocrine effects distally Promotes pre adipocytes to become mature fat cells with increase in size Serum adiponectin levels are lower with weight gain and higher with weight loss Also exerts anti inflammatory properties

16. Leptin Adipokine involved in energy homeostasis Structural homology similar to other cytokine proteinssuch as TNF alpha and IL-6 Exerts main effect via satiety centres

17. Thank you