1. THE HISTORY OF THE MICROSCOPE

2. EARLY MICROSCOPES
The first “microscopes” consisted of merely a tube with a plate for the object at one end and a lens which gave a magnification less than ten times the actual size.

3. Anton van Leeuwenhoek 1632-1723 Apprentice, non-educated.
Worked on improving lenses to the magnification of up to 270 times
Considered the father of microscopy.

4. Anton van Leeuwenhoek
He is credited for seeing/describing the first bacteria, yeast, plants, the teeming life in a drop of water, and the circulation of the blood in the capillaries.

5. POND WATER!!

6. Robert Hooke
Re-confirmed and improved on the design of the microscope.
Coined the word “cell”
Well educated.

7. Types of Microscopes
Compound Microscopes
Electron Microscopes

8. COMPOUND MICROSCOPE Compound Light Microscopes are light illuminated.
Two dimensional
Very common
High Magnification
Low Resolution
This is what we use!

9. HYDRA- Seen with a Compound Microscope

10. Electron Microscope Electron illuminated. This gives a 2D or 3D view.
Thin slices of specimen are obtained. The electron beams pass through this.
High magnification and high resolution.

11. Can you guess?
A cat flea…
Magnified 300 times

12. Can you guess?
Deer Tick
Magnified 500 x

13. Can you guess?
The foot of the common housefly
Magnified 300 x

14. Magnification Your microscope has 3 magnifications Low, Med, and High
Each objective will have a specific magnification. (Low-4, Med-10, High 40)
In addition to this, the ocular lens (10 X) the magnification
The total magnification is the multiple of the objective times the ocular lens.

15. Magnification of the Compound Microscope
Magnification
Ocular lens
Total
Low Power 4x
10x
40x
Medium Power
100x
High Power
400x

16. Microscope!
When looking at an object through the microscope…keep in mind that the image is actually inverted!
We will observe this today when working on your microscopes!

17. Using the Microscope Place the Slide on the Microscope Use Stage Clips
Click Nosepiece to the lowest (shortest) setting
Look into the Eyepiece
Use the Coarse Focus
Give students a slide from the “common things” set, each student will practice focusing and changing objectives.

18. Using High Power Bring slide into focus
You will then click to the nosepiece to medium power.
Do NOT use the Coarse Focusing Knob ONLY use the Fine Focus Knob to bring the slide to view
Repeat steps with the high power.
Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.
What can you find on your slide?

19. Microscope Care & Handling

20. Microscope Care
Always carry with 2 hands; one hand on the arm and one hand on the base.
Only use lens paper for cleaning
Do not force knobs
Always store covered
Keep objects clear of desk and cords
Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.

21. Microscope Parts Eyepiece Body Tube Revolving Nosepiece Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Diaphragm
Fine Focus
Light
Base

22. Microscope Care and Handling
How do I transport the microscope?
Again..the proper way to carry a microscope is with one hand on the arm and one hand on the base.

23. Microscope Care and Handling
How much does a microscope cost? $
What happens if I break it?
If you were engaging in any horse play or not following lab regulations YOU will be responsible for paying for any damages!!

24. Microscope Care & Handling
Clean all slides, materials, and work area when you're done. Please, be careful with the slides and cover slips. They are made of glass and if broken, you will get cut and you will bleed.
DON'T CUT YOURSELF, THERE ARE NO BAND AIDS IN THIS ROOM…Maybe I have a few!!!