1. 440-LC Fraction Collector
P. van Poppel – Field Support Specialist
October 2009

2. Overview Introduction to 440-LC Operation and Control Installation
Options
Usage
Operation and Control
Installation
Problems
440 is related to SPS3 auto sampler used in AA, ICP-ES etc. Most of the parts are the same. Main board is also same except for firmware.

3. 440-LC Fraction Collector
Theta axis
Z axis slide
Retaining ring
Probe mounting block
Ridged mount nut on the probe assembly
Ferrule holding the metal probe
Probe
Rack
Rack location mat
Simple installation through RS232. Requires calibration of all positions. This is important, as the rack location mat is a little flexible and the tube positions are not set in concrete.. Requires delay volume determination to ensure best decision making in timing.

4. 440-LC Fraction Collector Options
High-flow kit including high-flow PEEK™ probe, diverter valve and funnel rack
High-flow probe
Funnel rack
USB to RS232 converter
Various racks:
90 position x 13 mm OD (7 mL) rack
60 position x 16 mm OD (13 mL) rack
40 position x 20 mm OD (25 mL) rack
24 position x 25 mm OD (38 mL) rack
21 position x 30 mm OD (50 mL) rack
Rack overlay for 40 position rack, 18 mm OD
Rack overlay for 60 position rack, 16 mm OD

5. Specifications Large fraction capacity
Wide flow rate range up to 800 mL/min
Compatible with range of HPLC detectors
Simple, intuitive software control
Compact footprint (600 x 400mm)
Diverter valve can handle up to movements before replacement is due.

6. Usage The Varian 440-LC is fully compatible with Galaxie software.
Real-time fraction collection marks are displayed

7. Control
Standalone control, no need for Galaxie. To operate manually or with third party software.

8. Installation Connect up power Connect serial cable between PC and F/C
Calibrate tray
Save coordinates
Install software
Determine delay time

9. Problems
Fractions not correlating to the tick marks on the Galaxie software
Liquid is not delivered to the tubes
Probe hits the top of the tubes or is not low enough
Error status LEDs are showing an error
The fraction collector shows offline in Status Overview
The arm is not moving correctly in the X, theta or Z axes
Fraction collector is not initializing
CPU activity LED (side of fraction collector) does not flash.
Host communications LEDs do not flash during communication