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© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann SPE-TT Tutorial Version
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 2 Introduction This document is a step-by-step guide for the usage of a SPE-TT system that elutes samples from SPE cartridges into the liquid handler. - Installation: Software & Hardware installation instructions (under construction). - Start-up: Start-up of an already installed instrument. - Transfer Process: Step-by-step description of a (series) of transfer. - Special Tasks: Insert Racks/ Supplied procedures and simple modifications of procedures/ Pooling of cartridges. - Trouble Shooting: Problems/Debug information. This is a Quick start guide! - Read the complete manual for details. - Installation & calibration of the Liquid Handler and Prospekt2 must be finished. This manual is based on - PrepGilsonST HyStar 3.2 (optionally SR1 or SR2)
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann SPE-TT Installation Under Construction
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 4 Software You must install PrepGilsonST and HyStar on the same computer. - Typically use the HyStar computer. When using the TopSpin computer note that you - must install PrepGilsonST and HyStar. - must have a sufficient number of COM ports. (1xLiquid Handler, 1xProspekt2, 2xLink = 4xCOM). If HyStar is already installed - do Help/About and check that is HyStar Version Do not install the Gilson215 Software from HyStar!!! - Open the windows control panel. Do add remove software. Select HyStar and do modify. Verify that the Gilson215 option is not selected. If HyStar is not installed - Install HyStar according to the general installation instructions. - Do not enable the Gilson215 option. - No special fraction collector license is required.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 5 Data Connections Connection of the LiquidHandler through - Preferably build in COM1 of the computer. - Additional COM-ports supplied by a PCI board mounted in the computer - Optionally by USB-RS232 converter. - Not by Ethernet-RS232 converter (Moxa NPort Server) Other instruments can use any kind of COM ports.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann SPE-TT Start-up
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 7 Prepare the Transfer in PrepGilsonST Open PrepGilsonST If the following window is shown, choose SPE-TT. If this windows is not shown - Check that the used configuration is SPE-TT. - See page 32 Change the configuration of how to select the SPE-TT application.Change the configuration If the liquid handler shows status yellow, do Service Tools/Initialize
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 8 Prepare the Transfer in PrepGilsonST For convenient access to instrument + racks press to move the arm. Connect the Prospekt2 to the T-piece at the needle. (Note: This connection might have been removed for other applications) Open the TubeBlock, and insert a sufficient number empty tubes. The solvent at the dilutor of the liquid handler should be the same as the solvent used for transfer in the Prospekt2. Note the position(s) of the racks with TubeBlock(s) and the empty tubes. In CleanProcedures do Purge system to remove air bubbles from dilutor and capillaries.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 9 Check the Trays – without Barcode reader With barcode reader racks are automatically detected. Skip this page!!! Open the configuration with View|Tray Check if the racks you want to use are available and correctly positioned. Move the mouse over the racks/trays to check for details (Note: lower part of the tooltip shows the properties of the selected zone i.e. where you clicked the last time with the mouse) Details of how to change he rack positions you find on page 23 Define Rack Positions manually.Define Rack Positions manually Stop
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 10 Define Parameters for Transfer in HyStar In HyStar open the Transfer Settings from the menu(in flow injection) or the Prospekt2 context menu (in Acquisition). Set the cartridge drying time - 1min for dried cartridges - 30min for loaded cartridges. Set Probehead wash&dry to 2min & 150µl Define the volume in the NMR tube as excess volume. - 3mm 40mm 190µl - 2.5mm 40mm 140µl - 2mm 40mm 80µl - SJ 1.7mm 23mm 30µl - For precise transfer set flow µl/min. Activate the option finalize!
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 11 Special Version for HyStar <3.1 only Define Parameters for Transfer in HyStar With HyStar 3.2 and later skip this page! In HyStar enter FlowInjection. Open the TransferSettings. Set the cartridge drying time - 1min for dried cartridges - 30min for loaded cartridges. Set Probehead wash&dry to 2min & 150µl. Enter the complete volume (volume to the Needle tip + volume in the NMR tube) - For example volume to needle tip=180µl - Volume for 2mm Tube 40mm 80µl - TransferVolume = 260µl - For precise transfer set flow µl/min. Activate the option finalize!
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann SPE-TT Transfer Process
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 13 Preparation I – Prepare Software Do Preparation and select Start Preparation Automation. The Create Orders dialog opens. Check for Created Orders - Typically it should be empty. - If there are orders left over from previous runs, check if you really want to execute them. If not … mark and delete them. Check the displayed cartridge trays. - Verify that the right trays are displayed. If not use the Select … tray buttons to load the correct trays files ?
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 14 Preparation II – Select Procedure/Cleaning Select the procedure for the transfer: - default_SPETT.gsp: Transfer only, no mixing. HyStar Versions <3.1 must use this procedure. - SPETT Mix 2_0mm Tubes.gsp SPETT Mix 2_5mm Tubes.gsp SPETT Mix 3_0mm Tubes.gsp: Mixing after transfer, liquid volume from HyStar must lead to 40mm filling height. - SPETT Mix 1_7mm 30ul.gsp: Mixing after transfer, liquid volume from HyStar must lead to 23mm filling height. For the first transfer an empty cartridge should be used for cleaning of the system. Identify a cartridge that can be used. - You can perform such a transfer in HyStar (If PrepGilsonST is not used, needle remains in the waste) or … - Follow the instructions on the next pages for the automated transfer. Choose only this cartridge, select one tube and press create the order before you continue the selection with the real cartridges.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 15 Transfer– Select Cartridges Select * ) cartridge(s) with the mouse. - Selected cartridges are shown in the upper window. - For a simple assignment of cartridge to tube, select one cartridge and finish the setup as describe below. - Cartridges are processed in the order of their position in the tray. For a user defined order, finish the setup for the 1 st cartridge as described below. Click on destination to select the NMR tube(s). More cartridges can be selected in a second round. *)Select cartridges with left click. Multiple selections with CTRL-click or by opening a rectangle around a range of cartridges.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 16 Transfer – Select Tubes Select * ) a sufficient number of tubes as destination for the selected cartridges. The selected tubes are assigned to the cartridges and displayed in the upper window. The first selected tube is assigned to the first cartridge in the list. For each selected cartridge you must select a tube. *)Select cartridges with left click. Multiple selections with CTRL-click or by opening a rectangle around a range of cartridges.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 17 Transfer – Create Orders Press Create Orders The list of cartridges & tubes in the upper window is converted into orders in the right window. PrepGilsonST returns to the Source selection. Repeat the process until the you have defined all cartridges, then leave the window with OK. Attention OK will start the Automation. - No further cartridge can be added to the list, before current cartridges are completely processed. - Depending on the drying time this can be a lengthy process!
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 18 Transfer – Verifying the Orders After closing the Create Order Files dialog the automation starts. Orders will be shown as white circles. First step is the test for valid parameters (volumes etc) which changes the color of the circles to purple. In case of problems the color turns to red and the automation stops. 1) The orders are normally sorted by the time of the Create Orders action. If more then one transfer was created at a time, the cartridges are sorted by their position in the tray. Check page 39 Potential problems – Order of TransferPotential problems – Order of Transfer
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 19 Transfer – Start the automation run After successful validation the liquid handler starts the automation. If HyStar is ready - A message appears. - The transfer starts. - No further actions are required. If HyStar is not ready, because the chromatography is still running - A message appears. - PrepGilsonST retries automatically every 60sec until HyStar is ready for transfer. - Ensure that HyStar automatically switches to the flow injection window after the chromatography. - When HyStar is in the flow injection window and ready for the transfer, the automation will continue normally.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 20 Transfer – Proceeding In the upper window of PrepGilsonST the progress can be observed: - Purple=Ready for transfer // Blue=Transfer running // Green=transfer done To remove filled tubes for NMR measurements - Do not remove tubes while the automation is running. The arm of the liquid handler can start moving at any time! - Do not press the Pause button. HyStar and PrepGilsonST may get out of synchronization. - Press the End after current order button, wait that the automation stops, remove the desired tubes, and restart the automation for the next schedules cartridges.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 21 Results Do File/Open Print Dialog and Save New … Preview. This will bring up a compact information of all orders For each order line you can get detailed information with the right mouse button.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann SPE-TT Special Tasks
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 23 Define Rack Positions manually Open the configuration with View|Configuration Click on the trays
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 24 New Rack I: Choose the position of the rack Check if the displayed racks corresponds to the current situation in the liquid handler If not … - Delete any wrong positioned racks with a double click. - For racks that contain parts you must first delete the contained tube blocks/ well plate and then delete the rack. To insert the new racks … - Click the right mouse button at the position where the rack is located. - Do Insert Barcode Simulation Data - Do not use Insert rack at selected site. With this procedure further definitions of rack properties are required.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 25 New Rack II: Insert empty racks For MATCH tubes(all sizes) choose Rack Code 348B. For SampleJet tubes(all sizes) choose Rack Code 205MI. Enter an unique ID for the Rack, for example MatchRack1. All racks in the system should have different names. Leave the rest unchanged and press OK. Repeat the procedure for other racks.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 26 Insert Tube Blocks in empty Rack I The previously inserted rack (348B or 205MI) with the two empty positions for the TubeBlocks is displayed in the tray. Click with the right mouse button the upper empty field and do Insert Barcode Simulation Data - Do not use Insert rack at selected site. With this procedure further definitions of rack properties are required.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 27 Insert Tube Blocks in empty Rack II Possible tube blocks (MATCH or SampleJet) will be shown. - Select the block with correct tubing size. Define a unique ID, for example TubeBlock1_2mm. All blocks in the system must have different names. Leave all other settings unchanged. Repeat the procedure for the 2 nd block.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 28 Supplied Procedures default_SPETT.gsp: All filling heights - Stays in drain during wash+dry of needle and dry of cartridge. - Goes to bottom 1 ) of selected source during transfer. - Returns to drain for next transfer. - No further actions. SPETT_Mix_2.0mm Tubes.gsp: Liquid level 40mm=80µl SPETT_Mix_2.5mm Tubes.gsp: Liquid level 40mm=140µl SPETT_Mix_3.0mm Tubes.gsp: Liquid level 40mm=190µl SPETT_Mix_1.7mm Tubes 30ul.gsp: Liquid level 23mm=30µl 1 ) - Stays in drain during wash+dry of needle and dry of cartridge. - Goes to bottom 1 ) of selected source during transfer. - Performs a mixing of the tube content after the transfer. - Returns to drain and cleans the needle, waits for the next transfer. - The mixing volume is fixed. Using smaller volumes in HyStar will cause the procedure to fail. Using higher sample volumes may result in inefficient mixing 1 )After 10mm the system checks for obstructions. The procedure will work with 1.5mm needle and without needle switch, however the detection only works with 0.5mm needle + needle switch. 2 )The 1.7mm procedure works with a lower filling height adapted to the 1.7mm (Cryo)Probe.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 29 Test the Transfer Procedure PrepGilsonST supplies a possibility to check if a transfer is possible. With the selected procedure, the current volumes in HyStar and the type of NMR tubes you can test the orders. - Do Preparation and select Start Verify Test all Orders Testloop. - Follow the instructions following page 15 Transfer– Select Cartridges identical to the normal setup procedure. Transfer– Select Cartridges - At the end PrepGilsonST will only verify the procedure but not start the transfer procedure. This allows you to check for errors. By running the standard Start Preparation Automation dialog - The above generated test orders can be deleted. - The above generated and tested orders can be started.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 30 Pooling of Cartridges I Pooling means to combine the liquid from several cartridges into one sample tube. Proceed as follows - Use CTRL-Click to select the cartridges that contain the same compound for example cartridges A4,A8,A12. - As destination assign the same tube for all cartridges by using CTRL-Click on the same tube. - To avoid confusion, do Create orders before you define the next set of cartridges CTRL-Click 3x times on the same tube
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 31 Pooling of Cartridges II Take care, that the selected tubes/containers are large enough to hold the complete volume of all transfer procedures. The mixing procedures are not very efficient in this case. The calculation for the mixing assumes the amount of liquid from one transfer in a tube.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 32 Change the configuration When opening PrepGilsonST a window is shown, where you can choose SPE-TT. If during start-up this windows is not shown: - do edit settings - reactivate the window - restart PrepGilsonST.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 33 Start Transfer during Chromatography The SPE-TT system allows you to start PrepGilsonST while HyStar is still running a chromatography. In this case the transfer into the tubes automatically starts after the end of the chromatography. In HyStar … - In Acquisition|Shutdown Settings, activate Switch to Flow Injection window. - Define the transfer settings in the icon of the Prospekt2 before you start the automation in PrepGilsonST. - For details check the HyStar documentation. In PrepGilsonST - Start the automation while the chromatography runs. - Select the cartridges which you expect to be filled during the chromatography. - A message indicates that the transfer is not started. - The system will automatically retry every 60sec. Once HyStar is in the flow injection window, the transfer will start.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann SPE-TT Trouble Shooting
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 35 Potential Problem – Timing/Volumes in HyStar The correct setup of HyStar is essential. Excess volume - You must define the volume from cartridge to needle tip as transfer volume. - You must define the liquid in the tube as excess volume. - Otherwise the mixing procedure may not be correctly performed. Finalize - You must activate the finalize option - Otherwise the timing calculations for the movement of the needle is incorrect. The needle may go into the sample tube while drying gas is still going through the needle. For both settings the same amount of liquid is delivered, but …. With this setting PrepGilsonST assumes 30µl in the tube and mixing will probably fail.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 36 Potential Problem – Rack/Block definition Note: Whenever a message like the following appears, you have tried to install a TubeBlock with same identifier two times. The ID must be a unique name! Change the name of the tube block/rack you want to insert. In this case for example TubeBlock2_2mm
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 37 Potential Problem – Tray file selection If a error message like the following appears, you have chosen a tray file from a non-standard directory. Use tray files only from the standard HyStar-Directory C:\Program Files\Bruker Daltonik\HyStar\Hardware\Prospekt2 (or the HyStar on your system, that is shown in the message)
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 38 Potential Problem – Volume too low Error during verification indicates wrong volumes. Right click for Error Info or Current Order Info for details. Example: Volume dispensed into the tube is too low for the mixing. Increase volume or use tube with smaller inner diameter. The minimum filling height for mixing is approx. 29mm (=2/3 of the standard volume)
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 39 Potential Problem – Order of Transfer Inside each order cartridges are sorted by the position in the tray. The sorting of the orders is define with - This order is defined with Edit/Settings - Choose: By Time of the Creation or By Order Name = Tray position of the cartridges. Example: To use 1H12 as first cartridge for cleaning of the system. - Select 1H12, assign tube 1, press Create Order. Select 1A1, 1A2, 1A1, 1E3, 1B8, assign tube 2..6, press Create order. Leave the Create orders dialog. - The transfer is done in the order [1H12], [1A1, 1A2, 1B8, 1E3]. - If you select 1H12, 1A2, 1A1, 1E3, 1B8, assign tubes 1…6, press Create Order. Leave the Create orders dialog, the order is [1A1, 1A2, 1B8, 1E3, 1H12].
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 40 Potential Problem – Too much cleaning Each time you start a automation process a cleaning procedure is executed to clean the system but also to ensure that the complete flow path is filled with liquid. If you transfer cartridge individually and not as sequence, you can disable the cleaning to save time and deuterated solvent. Do disable the cleaning completely do Edit/Settings. - You can disable the Cleaning procedure at the beginning and/or end of the transfer. In this case you should perform a clean needle manually before the first cartridge transfer.
© Bruker BioSpin ; File: Tutorial 11 - SPE-TT; Version: ; By: Dr. Ulrich Braumann 41 Potential Problem – Needle does not fit The 0.5mm needle is supplied with a sensor, that detects if the needle inserts correctly into the tube/container. For this purpose the needle moves 20mm down and checks for any obstructions and then goes to the bottom of the tube. If the needle cannot move into the tube - The needle moves up and a second attempt is done. - If this also fails the automation is aborted. - As it is not possible to automatically abort the transfer in HyStar at this stage, the needle remains at the current position (on top of the tube). - By this you have a good chance to recover the sample from the cartridge. - All further cartridges are not transferred.
© Bruker BioSpin 2008; File: braumann_jena_ ppt; Version: ; By: Dr. Ulrich Braumann LC-(SPE)-(Cryo)NMR(/MS) as Routine Analysis Technique:
EasyPreparation with Barcode Reader. EasyPreparation with Barcode Reader :19 This tutorial describes the application “Easy preparation“
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