1. About Science Prof OnlineScience Prof Online PowerPoint Resources Science Prof Online (SPO) is a free science education website that provides fully-developed Virtual Science Classrooms, science-related PowerPoints, articles and images. The site is designed to be a helpful resource for students, educators, and anyone interested in learning about science. The SPO Virtual Classrooms offer many educational resources, including practice test questions, review questions, lecture PowerPoints, video tutorials, sample assignments and course syllabi. New materials are continually being developed, so check back frequently, or follow us on Facebook (Science Prof Online) or Twitter (ScienceProfSPO) for updates. Many SPO PowerPoints are available in a variety of formats, such as fully editable PowerPoint files, as well as uneditable versions in smaller file sizes, such as PowerPoint Shows and Portable Document Format (.pdf), for ease of printing. Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in slide show mode to use the hyperlinks directly. Several helpful links to fun and interactive learning tools are included throughout the PPT and on the Smart Links slide, near the end of each presentation. You must be in slide show mode to utilize hyperlinks and animations. This digital resource is licensed under Creative Commons Attribution-ShareAlike 3.0: http://creativecommons.org/licenses/by-sa/3.0/ Alicia Cepaitis, MS Chief Creative Nerd Science Prof Online Online Education Resources, LLC alicia@scienceprofonline.com Image: Compound microscope objectives, T. Port Tami Port, MS Creator of Science Prof Online Chief Executive Nerd Science Prof Online Online Education Resources, LLC info@scienceprofonline.com From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com

2. Diversity of Life: Protista Images: Ceratium, Tabellaria, Cyclidium, Volvox, and Diatoms, by T. Port; Amoebae, WikiAmoebae From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com

3. Two Basic Types of Cells Eukaryotic Cells Prokaryotic Cells Images: Prokaryotic cell diagram & Eukaryotic cell diagram, M. RuizProkaryotic cell diagram Eukaryotic cell diagram From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com Cells: -are the building blocks of life! -All living things are made of one or more cells. -only come from other cells. -are, really small. How small are they?How small are they? -small because of surface to volume ratio

4. Classification of Kingdoms of Eukaryotes MoneraProtistsFungiPlantsAnimals Prokarytic or Eukaryotic? Cell Organization (single or multicellular?) Examples of Organisms Distinguishing Characteristics Other Details Name three ways in which eukaryotes and prokaryotes differ: From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com

5. Classification of Organisms by Kingdom Eukaryotes Prokaryotes Image: Phylogenetic Tree, WikiPhylogenetic TreeFrom the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com Monera

6. Protista Life in a Drop of Water VIDEO LECTURE: Diversity of ProtistsDiversity of Protists Images: Clockwise from top: Ceratium, Tabellaria, Cyclidium, Volvox, and Diatoms, by T. Port; Amoebae, Wiki. Water drop, WikiAmoebaeWater drop From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com Eukaryotic Unicellular Need moist environment Some more plant-like (autotrophs) Some more animal-like (heterotrophs) Some are more fungus-like (saprophytes)

7. Three Categories of Protists Plant-like Autotrophs (Self-feeders, photosynthetic) Algae Proto-plants Diatoms Glass-like shells Dinoflagellates Plankton Euglenoids Move like animals, photosynthesize like plants Animal-like Heterotrophs (Eat other living things) Pseudopods false feet Flagellates move using flagella Ciliates move using cilia Fungus-like Saprophytes (Eats dead, decaying matter) Slime Molds Move like animals, absorb food like fungi Water Molds often parasites, caused Irish potato famine LECTURE VIDEO: Kingdom Protista

8. Plant-like Protists: Algae, Dinoflagellates, Diatoms, Euglenoids Images: Tabellaria, Diatoms, by T. Port; Volvox, Wiki, Euglena. Volvox From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com -Photosynthetic -Source of most O 2 on earth. - Base of the aquatic food chain. Ceratium Dinoflagellate Tabellaria Diatom Volvox Algae Euglena Motile, photosynthetic Euglenoid Autotrophic Plankton

9. Animal-like Protists: Protozoans From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com Images: Paramecium, Wiki; Amoebae, Wiki, Rotifer, Wiki; Giardia, CDCParameciumAmoebae Rotifer  Generally defined by four characteristics: - EukaryoticEukaryotic - Single-celled (unicellular) - No cell wall - Motility (nearly all are able to move due to cilia, flagella or pseudopodia)  Require moist environments.  Most are free-living and harmless. Very few are pathogens.  Motile feeding stage called trophozoite.  Many have hardy resting stage called cyst.

10. Image: Textbook, Microbiology, with Diseases by Taxonomy, R. Bauman.Microbiology, with Diseases by Taxonomy Protozoan Life Cycle Q : What prokaryotic survival skill does this remind you of? prokaryotic From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

11. Images: Coleps, Wiki, Cyclidium, T. Port; Volvox, Wiki Amoebae, Wiki, Rotifer, Wiki, Stentor, Wiki, Gastrotrich, WikiColepsVolvoxAmoebaeRotiferStentor From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com Cyclidium Ciliate Amoebae Pseudopod Rotifer Ciliate Gastrotrich Related to nematodes (roundworm & flatworms) Coleps Cilia Stentor Ciliate

12. Some Protozoans of Interest to Medical Microbiologists From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com Image: Naegleria folwleri, WikiNaegleria folwleri From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com

13. Pathogenic species that feed off the intestinal lining. Infection results from eating food / drinking water contaminated by the organism - usually from feces. A large number generates inflammation, which causes nausea, stomach ache and diarrhea. Chronic infection may result in malnourishment, blocking absorption of food across their intestinal wall. Protozoan Species: Giardia lamblia (aka G. intestinalis) Images: Public Health Image Library,(PHIL) #11649; #11632 & #3394PHIL) From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com As a puppy, my dog Lulu was diagnosed with Giardia. The vet ordered an ELISA test on a stool sample. Unlike a routine flotation stool check that gets parasite eggs and cysts to float to the top of a solution, the ELISA test looks for a specific antigen (or protein) of the Giardia organism. Giardiasis is typically treated over several days with the drug Metronidazole.Giardia

14.  Inflammatory disorder of the intestine, especially the colon, that results in severe diarrhea containing mucus and/or blood in the feces.  Untreated, dysentery can be fatal due to massive dehydration.  Can be caused by bacteria, protozans or parasitic worms. Image: Source Unknown Disease, Please : Dysentery From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

15. A type of amoebae. Eat and move by extending parts of their bodies to form pseudopods (SUE-dough-pods). Exists asymptomatically in 10% of world’s population. When disease develops, can be fatal (kills 100,000 annually). Feeds on the lining of the gut. Irritation created can lead to condition known as amoebic dysentery. Contracted by eating or drinking fecally contaminated food or water. Protozoan Species: Entamoeba histolytica (ENT-ah-MEE-bah HISS-tow-LIT-ick-ah) Image: E. histolytica spore, CDC; E. histolytica life cycle, Mariana Ruiz; E. histolytica trophozoite, CDCE. histolytica sporeE. histolytica life cycleE. histolytica trophozoite, From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

16. Meningoencephalitis caused by the amoeba Naegleria fowleri (nuh-GLEER-ee-uh FOWL'-erh-eye), a parasitic microorganism that feeds on brain tissue. How Common is N. fowleri? Infection very rare (34 US cases in past 10 years, but nearly always fatal. Cases most often occur during the dry, hot summer months, when water is warm and at low levels. How N. fowleri Attacks Enters body through the nose; invades CNS by penetrating the olfactory mucosa and nasal tissues. Early infections: necrosis (tissue death) and hemorrhaging in the olfactory bulbs. Amoeba then climbs along nerve fibers through the floor of the cranium, into the brain. Where Is Naegleria Found? Worldwide distribution, typically found in warm fresh water, temperatures ranging from 77 – 95 F. How to Reduce Your Risk Infection usually follows water-related activities (swimming underwater, diving, or any water sport that results in water going up the nose). Infection can only result from exposure to the amoeba ’ s environment, not from person-to- person contact. Protozoan Species: Naegleria fowleri Image: Naegleria folwleri, WikiNaegleria folwleri From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com

17. Caused by parasite Toxoplasma gondii. Can get from contaminated water, eating undercooked infected meat (especially pork, lamb & venison), or contact with cat feces that contain Toxoplasma. 1/3 of the global population and 60 million people in US may be infected, but few have symptoms. Mild symptoms are flu-like. Pregnant women and people with compromised immune systems could develop serious health problems. Severe toxoplasmosis can damage the brain, eyes and other organs. Severe cases are more likely in individuals who have weak immune systems. A leading cause of death attributed to foodborne illness in the United States. Images: Toxoplasmosis life cycle, Wiki; Toxoplasma gondii, WikiToxoplasmosis life cycle Toxoplasma gondii Disease, Please : LISTEN: Radiolab segment on Toxoplasma, “The Scratch”The Scratch

18. Life-threatening disease caused by Plasmodium protozoan parasite transmitted through the bites of infected mosquitoes. Symptoms include fever, chills, and flu- like illness. Left untreated can cause coma and death if progresses to cerebral malaria. ~ 198 million cases of malaria in 2013. In 2013, malaria caused an estimated 584 000 deaths, mostly among African children. Images: Anopheles mosquito, Malaria Parasite Life Cycle, WikiAnopheles mosquitoMalaria Parasite Life Cycle From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com Disease, Please : Malaria Increased malaria prevention and control measures are dramatically reducing the malaria burden in many places. ~ 1,500 cases diagnosed in US each year. Most are travelers and immigrants returning from countries where malaria commonly occurs (mainly sub-Saharan Africa and South Asia). Travellers from malaria-free areas are very vulnerable to the disease.

19. Fungus-like Protists: Slime & Water Molds From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com Images: Fuligo septica slime mold; Water mold from a stream WikiFuligo septica Water mold Slime & Water Molds are saprophytes: Decomposers; recycle nutrients Mobile stage of life cycle. Spores have cilia. Slime Molds Move like animals, absorb food like fungi Large, ~1 meter; single-celled mass of cytoplasm. Water Molds often parasites, caused Irish potato famine VIDEO: You don’t need a brain to be intelligent – Slime MoldYou don’t need a brain to be intelligent – Slime Mold Fuligo septica The “Dog Vomit” Slime Mold Water mold from a stream

20. Animal CellAnimal Cell (Eukaryote) Images: Animal cell diagram, M. RuizAnimal cell diagram From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com

21. Confused? Here are some links to fun resources that further explain the biology of kingdom Protista: Diversity of Protists, video from JBF School.Diversity of Protists Kingdom Protista, Lecture Video from Beverly BiologyKingdom Protista, Lecture Video You Don’t Need a Brain to Be Intelligent – Slime mold, video from PBSYou Don’t Need a Brain to Be Intelligent – Slime mold Old & Odd: Bacteria, Archaea & Protists, from Crash Course Biology.Old & Odd: Bacteria, Archaea & Protists Life in a Drop of Water, from Rainbow Educational Media.Life in a Drop of Water Eukaryotic Cells Main Page on the Virtual Cell Biology Classroom of Science Prof Online.Eukaryotic Cells Science Prof Online Eukaryotic Cell: Structures, Functions & Diagrams, an article from SPO.Eukaryotic Cell (You must be in PPT slideshow view to click on links.) From the Virtual Biology Classroom on ScienceProfOnline.comScienceProfOnline.com