1. Cystocentesis Culture

2. The bladder is palpated and immobilized. The bladder should not be squeezed tightly as the puncture is being made as this can cause urine to leak from the puncture site into the abdominal cavity The needle should be inserted at a 45 degree angle to the bladder. Create negative pressure by pulling back on the plunger of the syringe. The needle should not be redirected if urine is not obtained, due to the risk of penetrating a bowel loop and subsequently taking the contaminated needle into the bladder or the bladder can be lacerated.

3. If a sample is not obtained on the first attempt, change the needle before making another attempt. If a sample is not obtained on 3 attempts, the bladder is probably small and in the pelvic canal. If a sample is obtained, the syringe plunger is released, and the needle is removed from the abdomen.

6. Male Cysto Cystocentesis can be performed on the midline in male dogs by retracting the penis and prepuce off the midline.

8. Animal Position: Standing or lateral Isolate the bladder with the palmar surfaces of your fingers. Apply steady firm pressure until urine is produced. Please be sure the animal’s bladder is full before you begin this method. Use CARE when expressing an overly distended bladder in the presence of urethral obstruction, as rupture may occur. Manual Bladder Expression