1. Photos in Living Color Purdue University iGEM Team Purdue University iGEM Team

2. Purdue University - iGEM Team Advisor : Jenna L. Rickus, Ph.D. Team Advisor : Jenna L. Rickus, Ph.D. Assistant Professor- Dept. of Agricultural & Biological Engineering & Weldon School of Biomedical Engineering Project Leader: John Schumm Project Leader: John Schumm Junior in Agricultural and Biological Engineering Graduate Supervisor: John Koehler Graduate Supervisor: John Koehler Master’s in Agricultural & Biological Engineering Aaron Young Aaron Young Sophomore in Biomedical Engineering Piyush Bajaj Piyush Bajaj Senior in Biomedical Engineering

3. Acknowledgements Agricultural and Biological Engineering Agricultural and Biological Engineering Purdue Resources in Engineering Purdue Resources in Engineering Prof. Jenna Rickus Prof. Jenna Rickus Prof. Kari Clase Prof. Kari Clase Prof. Nathan Mosier Prof. Nathan Mosier Bindley Bioscience Center Bindley Bioscience Center Purdue Laboratory of Renewable Resources in Engineering Purdue Laboratory of Renewable Resources in Engineering

4. Purdue University History Founded in 1869 and named after benefactor John Purdue, Purdue University began its journey with six instructors, 39 students and a mission to provide agriculture and mechanic arts education. History Founded in 1869 and named after benefactor John Purdue, Purdue University began its journey with six instructors, 39 students and a mission to provide agriculture and mechanic arts education. Student Body West Lafayette enrollment of 38,712 students (fall 2005); students from 50 states and 130 countries. Student Body West Lafayette enrollment of 38,712 students (fall 2005); students from 50 states and 130 countries. Purdue University enjoys global renown for its world-class instructional and research pursuits. Purdue University enjoys global renown for its world-class instructional and research pursuits.

5. iGEM & Purdue As one of the largest universities in the nation, Purdue is acclaimed for the quality of its teaching and research in a wide range of fields. As one of the largest universities in the nation, Purdue is acclaimed for the quality of its teaching and research in a wide range of fields. Participating for the first time in the iGEM competition. Participating for the first time in the iGEM competition. Formation of a student organization titled Biological Engineering in Genetics. Formation of a student organization titled Biological Engineering in Genetics.

6. In the Beginning… BEG is a completely student run organization and an officially recognized Purdue club. BEG is a completely student run organization and an officially recognized Purdue club. To gain such status, a constitution was written to govern the club. To gain such status, a constitution was written to govern the club. This included officer elections, lab safety, and other foreseeable issues. This included officer elections, lab safety, and other foreseeable issues. Completed and submitted to Purdue for approval and status. Completed and submitted to Purdue for approval and status. Makes Purdue BEG unique and appealing to donors as it is student run and directed. Makes Purdue BEG unique and appealing to donors as it is student run and directed.

7. Did Somebody say Money? Fundraising conducted within school departments to pay for research, travel, and other associated costs. Fundraising conducted within school departments to pay for research, travel, and other associated costs. Packet of information developed and distributed during sales pitch. Packet of information developed and distributed during sales pitch. Samples of used material will be available on the BEG website for future reference by other schools. Samples of used material will be available on the BEG website for future reference by other schools. Referencing student basis, students have control of funds but cannot create expenditures without approval of Advisor and Treasurer. Referencing student basis, students have control of funds but cannot create expenditures without approval of Advisor and Treasurer.

8. The Idea Fluorescent Biological Photo Paper Fluorescent Biological Photo Paper Use ink Jet printer to apply repressors/inducers to sheet of paper. Incubate Paper with Genetically Modified e-coli that express fluorescent colors in presence of these repressors/inducers.

9. Part Priorities Use water soluble molecules. Use water soluble molecules. IPTG and tetR IPTG and tetR Use an antibiotic to kill e-coli in white space. Use an antibiotic to kill e-coli in white space. Kanamycin Kanamycin Signal for strong colored fluorescent proteins Signal for strong colored fluorescent proteins GFP, mRFP GFP, mRFP

10. Part Selection Three Parts used Three Parts used GFP BBa_l13521 GFP BBa_l13521 RFP BBa_j04430 RFP BBa_j04430 tetR Plasmid tetR Plasmid

11. Part Creation- Standard Assembly

12. The Process Prepared Competent Cells Prepared Competent Cells Transformation of DNA Transformation of DNA Miniprep Miniprep Enzyme Digest Enzyme Digest Gel Electrophoresis Gel Electrophoresis Gel Extraction Gel Extraction Ligation Ligation Repeat Repeat

13. Printer Problems Loading Problems Loading Problems Ink/ Promoter Viscosity Ink/ Promoter Viscosity Diffusion throughout the paper Diffusion throughout the paper Printer Solutions Find an easily refillable printer Print trial with table sugar

14. Biological Problems Bacteria Growing on Paper Bacteria Growing on Paper Will it stay in one place? Will it stay in one place? Diffusion of Promoters in media Diffusion of Promoters in media Resolution of GFP mRFP Resolution of GFP mRFP

15. Future Plans Recruit full time student research over the summer Recruit full time student research over the summer 3-5 Students 3-5 Students Use the Purdue SURF program Use the Purdue SURF program Expand into other engineering disciplines Expand into other engineering disciplines

16. iGEM plans Keep the project student run Keep the project student run Allow the next generation of students to pick their project Allow the next generation of students to pick their project Encourage members with little experience to join Encourage members with little experience to join Obtain dedicated lab space and equipment Obtain dedicated lab space and equipment

17. Funding Need a reliable source of annual funding Need a reliable source of annual funding Lab equipment, trips, summer students Lab equipment, trips, summer students Goal is an endowment through Discovery Park Goal is an endowment through Discovery Park

18. Questions