1. Central Nervous System Histology Slide Orientation1

2. Spinal cord Dorsal horns: Interneurons Ventral horns: Motor neurons
Lateral horns:
Sympathetic neurons Parasympathetic (S2-4) neurons
DRG: Sensory (pseudounipolar) neurons
Autonomic ganglia: Post ganglionic neurons with unmyelinated axons. Sympathetic: paravertebral ganglia Parasympathetic: In organs

3. 65-2 Lumbar Spinal Cord, H&E
Dura mater
D.R.G.
DRG=dorsal root ganglia
Spinal Cord
Myelinated nerves

4. #65-1N Spinal cord and DRG Dorsal horn Lateral horn Ventral horn
Dorsal root ganglion (DRG)

5. Dorsal nucleus of Clarke
66a Thoracic Spinal Cord
Dorsal median sulcus
Dorsal nucleus of Clarke
(proprioceptive)
Dorsal horn
Lateral horn
Ventral horn
Ventral median fissure

6. 66a Thoracic Spinal Cord Neurons in Lateral Horn
Neurons in ventral Horn

7. 65-1N Motor neurons, glial cells and neuropil
Note: the “glial cells” are astrocytes, oligodendrocytes, and/or microglia, which are typically very difficult to distinguish in routine stains of normal tissue. In response to injury, there is marked proliferation of reactive astrocytes, a phenomenon known as “gliosis,” in which case it would be pretty safe to ASSUME the glial cells seen are mostly astrocytes. However, the only way to know FOR SURE would be an immunohistochemical stain.
Neuropil

8. Dorsal root ganglion cells

9. #74 Sympathetic ganglion cells #75 Parasympathetic ganglion cells
Seminal vesicle

10. Sensory and Autonomic Neurons (DRG) (Parasympathetic)

11. Brain Cerebrum Cerebellum 3rd ventricle 4th ventricle Pons
Sulcus (sulci) Gyrus (gyri)
3rd ventricle
Cerebellum
4th ventricle
Pons
Medulla oblongata

12. Cerebrum and Cerebellum77

13. #77 Cerebellum #76b Cerebrum
C: Cortex of gray matter W: White matter C C C W
C=cortex
W=white matter W W

14. #77 Cerebellum
Cortex
White matter

15. #77 Cerebellum ML GL WM Purkinje neurons (cells)
ML=molecular layer WM=white matter
GL=granule cell layer WM
Purkinje neurons (cells)

16. #77a Cerebellar Cortex

17. #76 Cerebrum
White matter
Cortex of gray matter

18. #76
Cerebral cortex
Ross and Pawlina, p.311

19. C C Cerebellum Cerebrum W W C: Cortex of gray matter W: White matter
Purkinje cells (neurons) C C
Pyramidal cells (neurons) W W

20. Hippocampal Formation
Posterior View
Lateral View

21. LIMBIC LOBE (Cortical Areas) Cortex Type
Cingulate gyrus & Isthmus Neocortex (six layers)
Subcallosal area
Parahippocampal Gyrus Paleocortex (3-5 layers)
Uncus
Hippocampus Archicortex (3 layers)
(Deep within parahippocampal gyrus)
“Hippocampus:” so-named because of it’s similarity to a sea-horse

22. Hippocampal Formation Dentate Gyrus Hippocampus Subiculum
Martin: Neuroanatomy
Figs. 15-3, 4, 14

23. #NP004N Hippocampal region CA: Cornus ammonis
Choroid plexus
CA2
Fornix
Lateral ventricle
CA3
Dentate gyrus
Hippocampus
CA1 through CA3 are specific regions of cell columns that run the length of the hippocampal formation. Since the hippocampus is curved, the columns are horn-shaped and called Cornu Ammoni (horns of Ammon) Here, the hippocampus is cut in coronal section, so the “columns” would be coming out at you)
CA1
Subiculum

24. 3 layers of Dentate gyrus Archicortex (3 layers), as opposed to Neocortex (6 layers)
Molecular layer
Molecular layer (dendrites of granule cells)
Granule cell layer (neurons)
Polymorphic layer
Polymorphic layer (interneurons)

25. 3 layers of Hippocampus (Archicortex)
Polymorphic layer
Pyramidal cell layer
Molecular layer
Pyramidal cells