1. Roger Coleman

2. Need Alzheimer’s disease costs patients, Medicare or other types of insurance agency's up to $148 billion dollars a year and for patients 65 and older it will triple healthcare costs. In America alone 5.3 million people live with Alzheimer's. Alzheimer's is also the seventh leading cause of death.

3. Need http://www.alzalliance.org/images/Forcast%20of %20Alzheimer%27s%20disease%20chart.gif Chart 1 shows statistics and future predictions of Alzheimer's disease

4. Knowledge Base Figure 1 shows C.elegans http://shirleywho.files.wordpress.com /2008/09/c-elegans_esa.jpg

5. Knowledge Base Figure 2 shows the lifecycle of c. elegans

6. Knowledge Base http://www.realage.com/health_guides/Alzheimer/i mg%5CLivingWithAlzheimers_artv2.jpg Picture 3 shoes a cross reference of a normal brain and a Alzheimer‘s infected brain.

7. Knowledge Base http://64.202.120.86/upload/image/news/g etting-out-of-the-loop-of-alzheimer-s- disease/amyloid-protein-loop.jpg Figure 4shows an amyloid b protein

8. Knowledge Base Chart 2 shows amaloid b protein product

9. Knowledge Base This picture shows other foods similar to Ginkgo Biloba that contain flavonoids. http://www.cyberline.com/juice/berry bg.JPG

10. Literature Review (Wu et, al, 2006) This shows the difference between a untreated amaloid b compared to a Egb-fed

11. Literature Review Ginko Biloba is well known for supporting brain function. (Colman 2001) Journal of the American Medical Association found that 120 mg of ginko can improve brain function. http://www.made-in- china.com/image/2f0j00DedaEMtgOzqoM/Ginkgo-Biloba- Leaf-Extract-CP2005-24-6-HPLC-.jpg

12. Literature Review (Ercoli, 2006 et al) found that significant improvement was shown by verbal and memory impaired patients using the ginko berry leaf http://www.herbalextractok.co m/HerbalExtract/Ginkgo_Bilob a_extract.jpg Figure 5 shows the plant of Ginkgo biloba

13. Purpose To determine if ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries has an effect on Alzheimer dieses in C. elegans. Null hypothesis is that no effect will occur when ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries ware added. The Alternative hypothesis is that ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries Hypothesis

14. Methodology The Effect of Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries on the suppression of Amaloid B in C. elegans C. Elegans (CL2006) strainf and given a 10 % solution of Ginkgo Biloba N=? C. Elegans ( CL2006 strain) will be given a 10% solution of Freeze dried Black raspberries N=? C. Elegans (CL2006 strain) will be given a 10 % solution of Freeze Dried Blueberries N= ? C. Elegans will be fed with 100 ul spots of e. coli (OP5O) on nematode growth medium and c. elegans will be cultured a 20 degrees C Results will be taken from visual analysis of C. elegans under a microscope. C. Elegnas ( Cl 2006 strain with no solutions N= ? (Control) C. Elegnas with amaloid b and ginkgo biblia solution (Experimental)

15. Protocols for Measurement Grown on NGM and C. elegans (control and CL2006) will be fed with E. Coli (OP5O) with 100 ul spots. Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries will be given in the NGM in a 10 % solution to both the CL2006 strain and the control strain

16. Protocols for Measurement (cont) All solutions will be given for one day at a time and analysis will be analyzed after 2 days. Analysis will be compared ( under a microscope) to the number of normal and the number of paralyzed c. elegans

17. Do- ability C. elegans and chemicals are easy to buy Much of the equipment is already in the lab Chemicals are easily applied to C. elegans

18. Budget Bulk Nutrition Ginkgo Biloba powder 8.99$8.99 Caenorhabditis Genetic Center University of Minnesota Caenorhabdi tid elegans CL2006 strain ? Based on lab code number ? Based on lab code number NutriFruitFreeze Dried Black Raspberry Powder 26-b8wl-f1ku$25. 95 NutriFruitFreeze Dried Blueberry Powder BT-4AXL- SRXH $20.95 Vendor Item Item Number Cost per unit Total Cost

19. Budget (cont) VendorItemItem Number Unit CostTotal Cost Carolina Biological Nematode Growth Agar 173520$6.25 (Quantity of 2) $12. 50 Carolina Biological Erwinia- Escherichia coli, living tube 155065$9.20

20. Bibliography Works Cited Hancock, Robert D. "Berry fruit as ?superfood?:." Scottish Crop Research Group 54 (2007). Http://www.iob.org/userfiles/File/biologist_archive/Biol_54_2_Hancock.pdf. Luo, Yuan. "Inhibition of amyloid -b aggregation and caspase- 3 activation by the Ginkgo biloba." Departments of Biological Sciences and Chemistry and Biochemistry (2002). Wilson, Mark A. "Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans." PubMed Central (2006). Wilson, Mark. "Bluebrry polyphenols increase the lifespan of c. elegans." Blackwell Publishing Ltd (2006). Wu. "Egb reduces ab specific pathological behaviors are suppressed by ginkgo biloba extract Egb 761 in caenorhabditis elegans." (2006). Yang, Huan. "The cytokine activity of HMGB1." Laboratory of Biomedical Science.