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William Kim Science Research – Period 4 Mrs. Flanagan.

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Presentation on theme: "William Kim Science Research – Period 4 Mrs. Flanagan."— Presentation transcript:

1 William Kim Science Research – Period 4 Mrs. Flanagan

2 Figure 1: Number of people age 65 and over, by age group, selected years and projected Need

3 Figure 2: Life expectancy at ages 65 and 85, by sex, selected years Need

4 Knowledge Base

5 Knowledge Base neural/celegans/celegans.gif /09/c-elegans_esa.jpg

6 Fig. 3 Blueberry polyphenols extend lifespan and slow aging in Caenorhabditis elegans. Literature Review Wilson et. al. 2006

7 Literature Review  James Joseph ( )  Tested and still testing fruit polyphenols and their effects on aging and the brain  Tested effects of blackberries, blueberries, strawberries, plum juice, and other berry fruits

8 Purpose Therefore the purpose of this experiment is to test the ability of blueberries, blackberries, and other berries in the prolonging of lifespan in C. elegans. Hypothesis Null Hypothesis: The blueberries, etc. will have no effect in prolonging the lifespan of C. elegans. Alternate Hypothesis: The blueberries, etc. will have a significant effect in prolonging the lifespan of C. elegans.

9 Polyphenols and Aging Methodology Caenorhabditis elegans Short lifespan of about 2-3 weeks, rapid generation time, and experiemental flexibilty Control Group Sample Size = 25 Not fed C. elegans medium with blueberry and other polyphenols Experimental Group Sample Size = 25 Fed crude blueberry extract (Vaccinium angustifolium) or a C18 column fraction containing bulk polyphenols in their medium Measurement Average pumping rate of pharynx contraction is recorded at days 8 and 10 of C. elegans life with the use of Motic Plus Camera attached to a stereo microscope. Statistical Analysis Statistical analysis by T-test, p<.05, will be used to determine if the collected data is significant.

10 Protocols for Measurement  Commercially prepared single strength wild blueberry juice (Vaccinium angustifolium) applied to a preconditioned C18 Column  C18 column washed with water to remove fructose, glucose, and organic acids of blueberries, then with 100% methanol to obtain the total polyphenolic fraction e=101&partNumber=WAT seaction/plants.plantdetail/plant_ID/17/index.htm

11 Protocols for Measurement  Lifespan scoring was initiated after hermaphrodites completed their final larval molt, on the first day of adulthood  Blueberry extracts were added to NGM agar plates of C. elegans on the first day of the lifespan assay  Pharynx contraction rate recorded with Motic Plus camera attached to a stereomicroscope


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