1. Copyright © 2010 Pearson Education, Inc. Connective Tissue Most abundant and widely distributed tissue type Four mature classes Connective tissue proper Cartilage Bone tissue Blood Mesenchyme is embryonic connective tissue Specializes to give rise to all mature classes of connective tissue Some mesenchymal cells remain as a source of new cells in mature connective tissue Gel-like ground substance with fibers and star-shaped mesenchymal cells

2. Copyright © 2010 Pearson Education, Inc.

3. Major Functions of Connective Tissue Binding and support Protection Insulation Transportation (blood)

4. Copyright © 2010 Pearson Education, Inc. Characteristics of Connective Tissue 1.Common origin (mesenchyme) 2.Varying degrees of vascularity Cartilage is avascular & dense is poorly vascularized. The rest are have an ample supply of blood vessels. 3.Extracellular matrix: cells separated by non-living extracellular matrix (ground substance and fibers) This matrix allows connective tissue to bear weight, withstand great tension & endure abuses (trauma and abrasion)

5. Copyright © 2010 Pearson Education, Inc. Structural Elements of Connective Tissue Ground substance Medium through which solutes diffuse between blood capillaries and cells Components: Interstitial fluid Adhesion proteins (“glue”) Proteoglycans Protein core + large polysaccharides (chrondroitin sulfate and hyaluronic acid) Trap water in varying amounts, affecting the viscosity of the ground substance

6. Copyright © 2010 Pearson Education, Inc. Structural Elements of Connective Tissue Three types of fibers Collagen (white fibers) Strongest and most abundant type Provides high tensile strength Elastic Networks of long, thin, elastin fibers that allow for stretch Reticular Short, fine, highly branched collagenous fibers

7. Copyright © 2010 Pearson Education, Inc. Structural Elements of Connective Tissue Cells Mitotically active and secretory cells = “blasts” Mature cells = “cytes” Fibroblasts in connective tissue proper Chondroblasts and chondrocytes in cartilage Osteoblasts and osteocytes in bone Hematopoietic (blood) stem cells in bone marrow Fat cells, white blood cells, mast cells, and macrophages

8. Copyright © 2010 Pearson Education, Inc. Figure 4.7 Macrophage Fibroblast Lymphocyte Fat cell Mast cell Neutrophil Capillary Cell types Extracellular matrix Fibers Collagen fiber Elastic fiber Reticular fiber Ground substance

9. Copyright © 2010 Pearson Education, Inc. Overview of Connective Tissues For each of the following examples of connective tissue, note: Description Function Location

10. Copyright © 2010 Pearson Education, Inc. Connective Tissue Proper Loose connective Areolar Adipose Reticular Dense connective Dense regular Dense irregular Elastic

11. Copyright © 2010 Pearson Education, Inc. (a) Connective tissue proper: loose connective tissue, areolar Description: Gel-like matrix with all three fiber types; cells: fibroblasts, macrophages, mast cells, and some white blood cells. Function: Wraps and cushions organs; its macrophages phagocytize bacteria; plays important role in inflammation; holds and conveys tissue fluid. Location: Widely distributed under epithelia of body, e.g., forms lamina propria of mucous membranes; packages organs; surrounds capillaries. Photomicrograph: Areolar connective tissue, a soft packaging tissue of the body (300x). Epithelium Lamina propria Fibroblast nuclei Elastic fibers Collagen fibers Figure 4.8a

12. Copyright © 2010 Pearson Education, Inc. Figure 4.8b (b) Connective tissue proper: loose connective tissue, adipose Description: Matrix as in areolar, but very sparse; closely packed adipocytes, or fat cells, have nucleus pushed to the side by large fat droplet. Function: Provides reserve food fuel; insulates against heat loss; supports and protects organs. Location: Under skin in the hypodermis; around kidneys and eyeballs; within abdomen; in breasts. Photomicrograph: Adipose tissue from the subcutaneous layer under the skin (350x). Nucleus of fat cell Vacuole containing fat droplet Adipose tissue Mammary glands

13. Copyright © 2010 Pearson Education, Inc. Figure 4.8c (c) Connective tissue proper: loose connective tissue, reticular Description: Network of reticular fibers in a typical loose ground substance; reticular cells lie on the network. Function: Fibers form a soft internal skeleton (stroma) that supports other cell types including white blood cells, mast cells, and macrophages. Location: Lymphoid organs (lymph nodes, bone marrow, and spleen). Photomicrograph: Dark-staining network of reticular connective tissue fibers forming the internal skeleton of the spleen (350x). Spleen White blood cell (lymphocyte) Reticular fibers

14. Copyright © 2010 Pearson Education, Inc. Figure 4.8d (d) Connective tissue proper: dense connective tissue, dense regular Description: Primarily parallel collagen fibers; a few elastic fibers; major cell type is the fibroblast. Function: Attaches muscles to bones or to muscles; attaches bones to bones; withstands great tensile stress when pulling force is applied in one direction. Location: Tendons, most ligaments, aponeuroses. Photomicrograph: Dense regular connective tissue from a tendon (500x). Shoulder joint Ligament Tendon Collagen fibers Nuclei of fibroblasts

15. Copyright © 2010 Pearson Education, Inc. Figure 4.8e (e) Connective tissue proper: dense connective tissue, dense irregular Description: Primarily irregularly arranged collagen fibers; some elastic fibers; major cell type is the fibroblast. Function: Able to withstand tension exerted in many directions; provides structural strength. Location: Fibrous capsules of organs and of joints; dermis of the skin; submucosa of digestive tract. Photomicrograph: Dense irregular connective tissue from the dermis of the skin (400x). Collagen fibers Nuclei of fibroblasts Fibrous joint capsule

16. Copyright © 2010 Pearson Education, Inc. Figure 4.8f (f) Connective tissue proper: dense connective tissue, elastic Description: Dense regular connective tissue containing a high proportion of elastic fibers. Function: Allows recoil of tissue following stretching; maintains pulsatile flow of blood through arteries; aids passive recoil of lungs following inspiration. Location: Walls of large arteries; within certain ligaments associated with the vertebral column; within the walls of the bronchial tubes. Elastic fibers Aorta Heart Photomicrograph: Elastic connective tissue in the wall of the aorta (250x).

17. Copyright © 2010 Pearson Education, Inc. Connective Tissue: Cartilage Three types of cartilage: Hyaline cartilage Elastic cartilage Fibrocartilage

18. Copyright © 2010 Pearson Education, Inc. Figure 4.8g (g) Cartilage: hyaline Description: Amorphous but firm matrix; collagen fibers form an imperceptible network; chondroblasts produce the matrix and when mature (chondrocytes) lie in lacunae. Function: Supports and reinforces; has resilient cushioning properties; resists compressive stress. Location: Forms most of the embryonic skeleton; covers the ends of long bones in joint cavities; forms costal cartilages of the ribs; cartilages of the nose, trachea, and larynx. Photomicrograph: Hyaline cartilage from the trachea (750x). Costal cartilages Chondrocyte in lacuna Matrix

19. Copyright © 2010 Pearson Education, Inc. Figure 4.8h (h) Cartilage: elastic Description: Similar to hyaline cartilage, but more elastic fibers in matrix. Function: Maintains the shape of a structure while allowing great flexibility. Location: Supports the external ear (pinna); epiglottis. Photomicrograph: Elastic cartilage from the human ear pinna; forms the flexible skeleton of the ear (800x). Chondrocyte in lacuna Matrix

20. Copyright © 2010 Pearson Education, Inc. Figure 4.8i (i) Cartilage: fibrocartilage Description: Matrix similar to but less firm than that in hyaline cartilage; thick collagen fibers predominate. Function: Tensile strength with the ability to absorb compressive shock. Location: Intervertebral discs; pubic symphysis; discs of knee joint. Photomicrograph: Fibrocartilage of an intervertebral disc (125x). Special staining produced the blue color seen. Intervertebral discs Chondrocytes in lacunae Collagen fiber

21. Copyright © 2010 Pearson Education, Inc. Epithelial Membranes Cutaneous membrane (skin) (More detail with the Integumentary System, Chapter 5) Mucous membranes Mucosae Line body cavities open to the exterior (e.g., digestive and respiratory tracts) Serous membranes Serosae Line internal body cavities (pleural membranes, visceral membranes, peritoneum etc.)

22. Copyright © 2010 Pearson Education, Inc. Figure 4.11a Cutaneous membrane (skin) (a) Cutaneous membrane (the skin) covers the body surface.

23. Copyright © 2010 Pearson Education, Inc. Figure 4.11b Mucosa of nasal cavity Mucosa of lung bronchi Mucosa of mouth Esophagus lining (b) Mucous membranes line body cavities open to the exterior.

24. Copyright © 2010 Pearson Education, Inc. Figure 4.11c Parietal pericardium Visceral pericardium (c) Serous membranes line body cavities closed to the exterior. Parietal peritoneum Visceral peritoneum Parietal pleura Visceral pleura

25. Copyright © 2010 Pearson Education, Inc. Tissue Injury Tissue injury = penetration of one of the bodies primary defense barriers (skin & mucosae) Injury triggers two types of responses: Inflammation: rapid, but not specific Immune: slower, but specific Tissue repair involves two major processes: Regeneration: replace damaged tissue with the same type of tissue Fibrosis: production of fibrous connective tissue called scar tissue Which process occurs depends on the location and severity of the tissue damage

26. Copyright © 2010 Pearson Education, Inc. Steps in Tissue Repair 1. Inflammation Trauma triggers the release of inflammatory chemicals from injured cells as well as macrophages & mast cells These chemicals trigger the dilation of blood vessels Dilation increases vessel permeability, allowing WBCs & plasma w/ clotting proteins and antibodies to seep into injured area Clotting occurs, stopping the loss of blood, holding edges of wound together and isolating it from bacteria, toxins etc.

27. Copyright © 2010 Pearson Education, Inc. Figure 4.12, step 1 Scab Blood clot in incised wound Epidermis Vein Inflammatory chemicals Inflammation sets the stage: Severed blood vessels bleed and inflammatory chemicals are released. Local blood vessels become more permeable, allowing white blood cells, fluid, clotting proteins and other plasma proteins to seep into the injured area. Clotting occurs; surface dries and forms a scab. Migrating white blood cell Artery 1

28. Copyright © 2010 Pearson Education, Inc. Steps in Tissue Repair 2. Organization and restored blood supply (beginning of actual repair) The blood clot is replaced with granulation tissue, which lays down a new capillary bed Fibroblasts within the granulation tissue produce growth factors, collagen fibers & contractile proteins to pull edges of wound together Epithelium begins to regenerate Debris is phagocytized by macrophages and underlying fibrous patch becomes scar tissue resistant to infection because it produces bacteria inhibiting fibers

29. Copyright © 2010 Pearson Education, Inc. Figure 4.12, step 2 Regenerating epithelium Area of granulation tissue ingrowth Fibroblas t Macrophage Organization restores the blood supply: The clot is replaced by granulation tissue, which restores the vascular supply. Fibroblasts produce collagen fibers that bridge the gap. Macrophages phagocytize cell debris. Surface epithelial cells multiply and migrate over the granulation tissue. 2

30. Copyright © 2010 Pearson Education, Inc. Steps in Tissue Repair 3. Regeneration and fibrosis The scab detaches as epithelial tissue regenerates Fibrous tissue matures; epithelium thickens and begins to resemble adjacent tissue Results in a fully regenerated epithelium with underlying scar tissue NOTE: In “pure infections” where there is no wound, puncture or scrape, repair is carried out by regeneration alone and there is generally not any clot formation or scarring.

31. Copyright © 2010 Pearson Education, Inc. Figure 4.12, step 3 Regenerated epithelium Fibrosed area Regeneration and fibrosis effect permanent repair: The fibrosed area matures and contracts; the epithelium thickens. A fully regenerated epithelium with an underlying area of scar tissue results. 3

32. Copyright © 2010 Pearson Education, Inc. Developmental Aspects of Tissues There are three primary germ layers: ectoderm, mesoderm, and endoderm Formed early in embryonic development (by the end of the second month) Specialize to form the four primary tissues Nerve tissue arises from ectoderm (mitosis basically stops after birth) Muscle and connective tissues arise from mesoderm (continue to divide after both) Epithelial tissues arise from all three germ layers (continue to divide after both) Mesoderm Endoderm 16-day-old embryo (dorsal surface view) Epithelium Nervous tissue (from ectoderm) Muscle and connective tissue (mostly from mesoderm) Ectoderm