1. IACUC TRAINING Update from AAALAC Assessment May 2009

2. Increase intensity of search strategies for alternatives to potentially painful procedures –Inclusion of relevant key words (painful or distressful procedures) to address alternatives –Rowland library assistance Gongchao Yang –Increased oversight by IACUC reviewers Search Strategies

3. Rodent Transportation Utilization of microisolator tops for transport of rats from vivarium to labs –Decrease allergen exposure to personnel –Improved protection of colony animals Microisolator tops located on racks with drapes Drapes also required Return tops to dirty cage wash and place used drape in hampers

4. HEPA filteration HEPA filtered vacuums –Lack of HEPA filtration system increases risk of allergen exposure –Use for animal prep –Use for cleaning chambers

5. Available from Lab Safety Supplies http://www.labsafety.com/search/dewalt+vacuum/24531455/

6. Microbial Monitoring Methods to monitor effectiveness of non-cage washed cage sanitation (non- standard cages) –Operant chambers, metabolic cages and chambers –Contact LAF to arrange testing Display animal care cards with requested information Care cards are located throughout the vivarium Place completed card on rack or room door

7. Aseptic Rodent Surgery Aseptic rodent survival surgeries –IACUC Guidelines available at: http://iacuc.umc.edu/document s/Guidelinesforrodentsurgery. pdf http://iacuc.umc.edu/document s/Guidelinesforrodentsurgery. pdf –Sterile instruments –Sterile gloves –Mask –Maintain sterile field

8. Anesthetic Vaporizer Service Vaporizer calibration and service Anesthesia maintenance –May be coordinated through LAF –Follow manufacturer’s recommendations –Typically annual evaluation

9. IACUC Policy Update Scavenging of Waste Anesthetic Gas – approved 12/18/08 http://iacuc.umc.edu/documents/ScavengingofWasteAnestheticGas. pdf http://iacuc.umc.edu/documents/ScavengingofWasteAnestheticGas. pdf Guidelines for the Use of Syringes and Needles – approved 12/18/08 http://iacuc.umc.edu/documents/needlerecappingguidelines.pdf http://iacuc.umc.edu/documents/needlerecappingguidelines.pdf

10. IACUC Policy Update Instructions for Validation of Autoclaves – 9/22/08 http://iacuc.umc.edu/documents/autoclavevalidation.pdf http://iacuc.umc.edu/documents/autoclavevalidation.pdf Guidelines for Rodent Survival Surgery – approved 4/21/09 http://iacuc.umc.edu/documents/Guidelinesforrodentsurgery.pdf http://iacuc.umc.edu/documents/Guidelinesforrodentsurgery.pdf Laser Use – contact Radiation Safety Office to receive clearance for laser use http://dor.umc.edu/RSO/rsocontactinfo.htmlhttp://dor.umc.edu/RSO/rsocontactinfo.html Guillotine maintenance – contact Mike or Tom @ 5-4511 (N307) to arrange maintenance – should occur on an as needed basis

11. Annual Occupational Health Update

12. Annual Update notification sent by email Access site at http://paws.umc.edu/EOH/login.jsphttp://paws.umc.edu/EOH/login.jsp Log in using Network User ID and password

13. Click on each form individually Heath Questionnaire → Animal Exposure → Update information and submit.