1. GENETIC ANALYSIS OF NITROGEN ACCUMULATION IN FOUR WHEAT CULTIVARS AND THEIR Desimir KNEZEVIC 1,-Aleksandar PAUNOVIC 2, -Milomirka MADIC 2, - Nevena DJUKIC 3 1 Faculty of Agriculture, Zubin Potok, University Pristina, Serbia, deskoa@ptt.yudeskoa@ptt.yu 2 Agricultural Faculty, Cacak, Cara Dusana 32, 32000 Cacak, Serbia 3 Faculty of Natural Sciences, Department of Biology Kragujevac, Serbia

2. - Firstly, cultivars creation : resistant to lodging, high yielding,, resistant to diseases, better technological quality, improved nutritive values The aim of cereal breeding are determined by requirements of producers and users and environmental conditions. -Aims of barley breeding are improvement: contents of amino acid β-glucans, high content of lysine -New technologies: gene transferring, development of biopharming -high adaptive value in different environmental conditions. -Cereals are important source charbochydrates, proteins, lipids, amino acids, mineralni elements, vitamins for human and animal nutrition and processing industry.

3. Example - World production and consumption of wheat Year Quantity in mil. metr. cent Area mil. hectars Consumption in mil. metr. cent Consumption in developing Countries (%) World Without East Europa and former SSSR 2001 583,9219,5590,650 % Rewiev of wheat production in 2000 year on the World ChinaIndiaLatin AmericaAsia (without Shina and India ) Količina u mil. metr. Cent. 111,926,520 The biggest wheat production on the World 1960-1970 Green Revolution 1. China, 2. India, 3. Turkey, 4. Pakistan, 5. Argentina

4. Example- World production and consumption of barley Year Quantity in mil. tons Area mil. hectars Consumption in mil. metr. cent Area thousand. hectars World Serbia 2005 159,865,5160140 Rewiev of consumption of barley on the World Animal feeding Bear production Human nutrition Total %65305100 Internationaln program of breeding ICARDA –3.9 mil. US$ -barley CIMMYT-27.5 mil. US$ -wheat Created 100 cultivars for sowing in 30 Countries CIMMYT-24.5 mil. US$ -kukuruz

5. World production of edible dry matter and proteins of plant species and their use for animal nutrition Plant spesies EDIBLE DRY MATTER (%) PROTEINS(%)Ekological origin Wheat2019 Mediterranean Barley75 Mediterranean Oat22 Mediterranean Rye11 Mediterranean Maize1813 Savanna Rice1711 Savanna Millet32 Medit/Savanna

6. ACHIEVEMENT OF MODERN BREEDING / based on genetic potential of new created cultivars improved growing technologies / FUTURE BREEDING - upkeep traditional breeding - Introduction of new technologies (use of plant as bioreactors in farmaceutical products, development of antibody and improvement of immunity to pathogenes). -marker tehnologies -Creation of high yielding and adaptive cultivars under the stress conditions. -transfers of gene Yield increasing – of wheat in last 5 decades was in ratio from 2000 kg ha -1 to 6000 kg ha -1 in Europe and USA In Mexico yield of wheat was increased from 750 kg ha -1 to 3200 kg ha -1 (cv. Penjamo 61 and Pitic 62) Created cultivars adapted to photoperiod sensitivity (photoperiodism) Earliness – adventage because of two harvest possibility Tolerant cultivars to: increasing of Al ++ concentration in soil, drought, low temperature and frost

7. Aim of this paper is study of variability wheat genotypes according to N accumulation and breeding of wheat with high capacity of N accumulatio and its importance for sustainable agriculture In the filed practices is very important optimize quantity of fertilizers, decrease expenses of production and improve efficiency of wheat plant of nitrogen absorption, accumulation and reutilization The nitrogen (N) plays main role in wheat nutrition because of its importance in protein and nucleic acid synthesis as well plant productivity. -Modern technology of wheat production mainly based on numerous scientific farming measures as well enormous application of mineral fertilizers. -Mainly one third of applied nutrient wheat plants are able use during vegetative period. In the filed practices is very important optimize quantity of fertilizers, decrease expenses of production and improve efficiency of wheat plant of nitrogen absorption, accumulation and reutilization.

8. USE OF NITROGEN The total N content represent indicator of N accumulation in plant which indicating root system activity and translocation of organic and inorganic matter to top of plant. Physiological N efficiency in plant indicating activity of top of plant and involve of absorbed N into processes of synthesis. On the base of dynamics N efficiency absorption and its introduction of organic synthesis are increasing speed of these processes and plant productivity. Genotype specificities of wheat N efficiency are reflect in all phases of plant growth, determined by general and variety specific genes located on three genomes (ABD) in hexaploid wheat (Gamzikova, 1994).

9. Table 1. Nitrogen accumulation (Nac) in wheat genotypes CultivarABCDF 1 hybrids Jugoslavija (A) 96.04106.8 +h 107.9 +h 108.0 d LSD 0.05 LSD 0.01 Osijecanka (B) 94.3 -pd 101.1108.8 +hd 106.2 +pd 16.8823.12 Zitnica (C) 104.0 +d 102.4 i 103.5120.9 +h NS Rana 2 (D) 96.6 d 91.6 -h 110.2 +h 108.8 F 2 hybrids LSD 0.05= 14.8LSD 0.01= 20.0

10. Table 2. Genetic variance and heritability for N accumulation (Nac) in wheat hybrids F1F1 F2F2 F1F1 F2F2 D198.004255.917 u 0.7720.858 H1H1 756. 58881.664v 0.228 0.142 H2H2 516.14438.2061.954 0.564 F204.796239.672K D /K R 1.72410.690 E68.54024.910*Th 2 61,50% H 2 / 4H 1 0.170 0.117*Gh 2 46,13% Variance N accumulation Variance N accumulation

11. Table 3. General combining abilities for N accumulation (Nac) YugoslaviaOsijecankaZitnicaNS R 2Se LSD 0.05 LSD 0.01 F1F1 Nac 4.2853.010 6.125* -10.822.6314.8426.921 Rank 2314 F2F2 Nac -1.4240.28610.36* -5.036 4.4668.214411.426 Rank 3214

12. Table 3. Specific combining abilities for N accumulation (Nac) Yugoslavia (A) Osijecanka (B) Zitnica (C) NS R 2 (D) Se LSD 0.05 LSD 0.01 A Nac 12.806-9.624 5.693 10.4621.1328.24 B Nac -2.511 -6.538-8.854 C Nac 0.988-3.65721.5796.1212.3616.562 D Nac -1.0821.174 7.128

13. Conclusions Developed genotypes by crossing Zitnica x NS Rana 2 cultivars is promising for sustainable agriculture growing, because of its low requirements for fertilizer application. Improvement of N accumulation in the plant leading to an increasing grain N content and yield is one of the major tasks of wheat breeding. Since N absorption from soils and translocation from roots and leaves to grains are primarily under genetic control. Breeders need make concept of creation new genotypes with high capacity of accumulation as well with high capacity of N uptake, utilization and translocation. Also, development of new genotypes with high capacity of N utilization will contribute to decreasing rate of application of nitrogen fertilizers what is very important for environmental protection.

14. What is necessary ? Go ahead without frustration Growing

15. VIEW AND PERSPECTIVE Mostly wheat breeders more than 50% active in government or public Institutions. -Use of adequately technologies for improvement of plant species and its products for users purposess. - Creation of cultivars for new purposes with improved quality for human and animal nutrition. Creation of cultivars with decreasing of losses and acceptance of risk of biotechnology and trangenic plants resistant to insects, diseades and herbicides. - Creation of cultivars with better ratio input/output -Creation of cultivars with significant higher yield than nowdays cultivars (5% and more), Breeders need use population of wild relatives existing cultivars germ-plasms induced mutation and methods of genetic engeenering

16. SUSTAINABILITY!?!