1. Small Talk Cell-to-Cell Communication in Bacteria

2. Prokaryotic (Bacterial) Cells

4. V. fischeri Quorum Sensing LuxI Luciferase Genes LuxR

5. Quorum Sensing In Bacteria LuxI Target genes LuxR

6. Acyl-Homoserine Lactone Autoinducers

7. LuxI-LuxR Quorum Sensing Systems V. fischeri P. aeruginosa A. tumefaciens E. carotovora Bioluminescence Virulence Factors, Biofilms Mating, Transfer of Mobile DNA Virulence Factors, Antibiotic Synthesis

9. LuxN LuxQ AI-1 AI-2 Luciferase Genes Light Quorum Sensing in V. harveyi

10. LuxN AI-1 AI-2 V. harveyi Reporter Strain AI-1 Luciferase Genes Light

11. LuxQ AI-1 AI-2 Luciferase Genes Light V. harveyi Reporter Strain AI-2

12. Quorum Sensing in E. coli and Salmonella

13. Actinobacillus actinomycetemcomitans Actinobacillus pleuropneumoniae Actinobacillus suis Bacillus anthracis Bacillus halodurans Bacillus subtilis Borrelia burgdorferi Campylobacter jejuni Clostridium acetobutylicum Clostridium difficile Clostridium perfringens Deinococcus radiodurans Escherichia coli MG1655 Escherichia coli O157:H7 Enterococcus faecalis Haemophilus influenzae Helicobacter pylori Klebsiella pneumoniae Lactococcus lactis Leuconostoc oenos Listeria monocytogenes Mannheimia haemolytica Neisseria gonorrhoeae Neisseria meningitidis Pasteurella multocida Pasteurella trehalosi Porphyromonas gingivalis Proteus mirabilis Salmonella paratyphi Salmonella typhi Salmonella typhimurium Shewanella putrefaciens Shigella flexneri Staphylococcus aureus Staphylococcus epidermidis Streptococcus gordonii Streptococcus mutans Streptococcus pneumoniae Streptococcus pyogenes Vibrio cholerae Vibrio harveyi Vibrio parahaemolyticus Vibrio anguillarum Vibrio vulnificus Yersinia pestis Bacteria That Contain luxS (A sample of 534 genomes sequenced)

14. Targets of AI-2 Actinobacillus actinomycetemcomitansPeriodontal virulence, Mixed-species biofilms Bacillus anthracisGrowth Bacillus cereusBiofilms Bacillus subtilisDevelopment Borrelia burgdorferiVirulence Campylobacter jejuniMotility, Toxins Clostridium difficileVirulence, Toxins Clostridium perfringensVirulence, Toxins Escherichia coli K12AI-2 transport, Biofilms, Cell division, DNA processing, Iron uptake Escherichia coli EHEC O157:H7Virulence, Type III Secretion, Motility Escherichia coli EPECMotility, Type III Secretion Haemophilus influenzaeVirulence, Cell invasion Helicobacter pyloriMotility, Biofilms Klebsiella pneumoniaeBiofilms Lactobacillus reuteriBiofilms in mice Listeria monocytogenesBiofilms Mannheimia haemolyticaVirulence, Encapsulation, Adhesion Neisseria meningitidisVirulence, Bacteremia Photorhabdus luminescensAntibiotic production Porphyromonas gingivalisVirulence, Proteases Hemin acquisition, Stress response Salmonella typhiBiofilms Salmonella typhimuriumAI-2 transport, Biofilms Serratia marcescensVirulence in C. elegans, Hemolysin, Antibiotic production Shigella flexneriVirB (virulence factor) Staphylococcus epidermisVirulence, Biofilms Streptococcus gordoniiMixed-species biofilms Streptococcus mutansVirulence, Biofilms, Competence, Stress response Streptococcus pneumoniaeVirulence, Persistence, Competence Streptococcus pyogenesVirulence factors, Protease, Hemolysin Vibrio anguillarumProtease Vibrio choleraeVirulence, Toxins, Biofilms Vibrio fischeriBioluminescence, Colonization Vibrio harveyiBioluminescence, Biofilms, Siderophore, Virulence, Type III Secretion, Protease

15. LuxN LuxQ AI-1 AI-2 Quorum Sensing in V. harveyi Luciferase Genes Light

16. 4,5-Dihydroxy-2,3-Pentanedione Inter-Species Communication The Structure of AI-2

17. LuxN LuxQ AI-1 AI-2 Luciferase Genes Light LuxS Making Antibiotics Based on AI-2

18. AI-2 Analog CHEMISTRY Making Antibiotics Based on AI-2

19. LuxQ AI-2 Luciferase Genes No Light AI-2 Quorum Sensing in V. harveyi

20. LuxN AI-1 Luciferase Genes Light AI-1 Quorum Sensing in V. harveyi

21. Virulent Bacteria Test Substance Inject Making Antibiotics Based on AI-2 Dead or Alive

22. Quorum Sensing In Bacteria Bacteria Talk to Each Other Bacteria Distinguish Self from Other Opportunities for Novel Biotechnological Applications To Impede/Enhance Quorum Sensing Controlled Functions Quorum Sensing Allows Bacteria To Be Multi-Cellular Similar to Higher Organisms Many More Molecules Remain To Be Discovered i.e., molecules that tell “who” the other is Natural Anti-Quorum Sensing Strategies Already Exist Multiple Languages i.e., intra- and inter-species communication