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BioFire (FilmArray) Multiplex PCR Assays

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Presentation on theme: "BioFire (FilmArray) Multiplex PCR Assays"— Presentation transcript:

1 BioFire (FilmArray) Multiplex PCR Assays
Teresa Finken, MT(ASCP)

2 Panels Available Respiratory Viral Panel
Blood Culture Identification Panel Gastrointestinal Panel

3 Methodology Multiplex Real-Time PCR Assay
These panels test for multiple targets simultaneously This methodology is real-time PCR meaning that it amplifies and detects at the same time

4 What does this Mean to You?
One sample One test Multiple results 1.5-hr turnaround time

5 Respiratory Viral Panel Targets
Viruses Adenovirus Coronavirus HKU1 Coronavirus NL63 Coronavirus 229E Coronavirus OC43 Human Metapneumovirus Human Rhinovirus/Enterovirus Influenza A Influenza A/H1 Influenza A/H1-2009 Influenza A/H3tapneumovirus Influenza B Parainfluenza 1 Parainfluenza 2 Parainfluenza 3 Parainfluenza 4 Respiratory Syncytial Virus Bacteria Bordetella pertussis Chlamydophila pneumoniae Mycoplasma pneumoniae

6 Blood Culture Id Panel Targets
Gram + Bacteria Enterococcus Listeria monocytogenes Staphylococcus Staphylococcus aureus Streptococcus Streptococcus agalactiae Streptococcus pyogenes Streptococcus pneumoniae Yeast Candida albicans Candida glabrata Candida krusei Candida parapsilosis Candida tropicalis Antibiotic Resistance mecA - methicillin resistant vanA/B - vancomycin resistant KPC - carbapenem resistant Gram – Bacteria Acinetobacter baumannii Haemophilus influenzae Neisseria meningitidis Pseudomonas aeruginosa Enterobacteriaceae Enterobacter cloacae complex Escherichia coli Klebsiella oxytoca Klebsiella pneumoniae Proteus Serratia marcescens

7 Gastrointestinal Panel Targets
Bacteria Campylobacter (jejuni, coli and upsaliensis) Clostridium difficile (toxin A/B) Plesiomonas shigelloides Salmonella Yersinia enterocolitica Vibrio (parahaemolyticus, vulnificus and cholerae) Vibrio cholerae Diarrheagenic E. coli/Shigella Enteroaggregative E. coli (EAEC) Enteropathogenic E. coli (EPEC) Enterotoxigenic E. coli (ETEC) lt/st Shiga-like toxin-producing E. coli (STEC) stx1/stx2 E. coli O157 Shigella/Enteroinvasive E. coli (EIEC) Viruses Adenovirus F 40/41 Astrovirus Norovirus GI/GII Rotavirus A Sapovirus (I, II, IV and V) Parasites Cryptosporidium Cyclospora cayetanensis Entamoeba histolytica Giardia lamblia

8 Sample Requirements Respiratory Viral Panel Blood Culture ID Panel
Nasal Wash Nasal Swab Sputum Respiratory specimen in Viral Transport Medium Gastrointestinal Panel Raw stool to be transferred to ETM Stool in ETM Blood Culture ID Panel Blood culture bottle that has been flagged positive by the Bacti Alert or Versatrek Analyzers

9 Positive Results Since Implementaion
These are just a few… Limited exposure to potentially hazardous bacterial isolates in blood cultures Faster treatment and more guided treatment of patients at the point of entry and after admission Quick diagnosis Faster antimicrobial therapy implementation to speed recovery and reduce morbidity Admitting a patient versus not admitting a patient

10 Questions…

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