1. William Terzaghi Spring 2014 Bio 398: Topics in Plant Biology

2. COURSE OVERVIEW 1) Understanding how plants work. 2) Understanding how plant biologists work. Method Technology

3. COURSE OVERVIEW 1) Understanding how plants work. 2) Understanding how plant biologists work. Method Technology http://www.nature.com/nature/journal/vaop/ncurrent/full/nature129 14.html

4. COURSE OVERVIEW 1) Understanding how plants work. 2) Understanding how plant biologists work. Method Technology http://www.nature.com/nature/journal/vaop/ncurrent/full/nature129 14.html http://www.cbc.ca/news/technology/older-trees-grow-faster-than- younger-ones-study-finds-1.2499298

5. Plan C We will pick a problem in plant biology and see where it takes us. 1.Biofuels 2.Climate/CO 2 change 3.Stress responses/stress avoidance Structural Biochemical (including C3 vs C4 vs CAM) Other (dormancy, carnivory, etc) 4.Plant products Defense compounds 5.Improving food production Breeding: new traits to pick & ways to find them GMO New crops 6.Biotechnology 7.Phytoremediation 8.Plant movements 9.Plant signaling (including neurobiology)

6. Plan C 1.Pick a problem

7. Plan C 1.Pick a problem 2.Pick some plants to study

8. Plan C 1.Pick a problem 2.Pick some plants to study 3.Design some experiments

9. Plan C 1.Pick a problem 2.Pick some plants to study 3.Design some experiments 4.See where they lead us

10. Plan C 1.Pick a problem 2.Pick some plants to study 3.Design some experiments 4.See where they lead us Grading? Combination of papers and presentations

11. Plan C 1.Pick a problem 2.Pick some plants to study 3.Design some experiments 4.See where they lead us Grading? Combination of papers and presentations Scavenger hunts?

12. Plan C Grading? Combination of papers and presentations First presentation:10 points Research presentation: 10 points Final presentation: 15 points Assignments: 5 points each Poster: 10 points Intermediate report 10 points Final report: 30 points Scavenger hunts?

13. BIO 398- Resource and Policy Information Instructor: Dr. William Terzaghi Office: SLC 363/CSC228 Office hours: MWF 12-1 in CSC228, T 1-2 in SLC 363, Thurs 1-2 in CSC228, or by appointment Phone: (570) 408-4762 Email: terzaghi@wilkes.edu

14. BIO 398 - Resource and Policy Information Instructor: Dr. William Terzaghi Office: SLC 363/CSC228 Office hours: MWF 12-1 in CSC228, T 1-2 in SLC 363, Thurs 1-2 in CSC228, or by appointment Phone: (570) 408-4762 Email: terzaghi@wilkes.edu Course webpage: http://staffweb.wilkes.edu/william.terzaghi/bio398.html

15. Vegetative Plants 3 Parts 1.Leaf 2.Stem 3.Root

16. Vegetative Plants 3 tissue types 1.Dermal 2.Ground 3.Vascular

17. Plant Development Cell division = growth

18. Plant Development Cell division = growth Determination = what cell can become

19. Plant Development Cell division = growth Determination = what cell can become Differentiation = cells become specific types

20. Plant Development Cell division = growth Determination = what cell can become Differentiation = cells become specific types Pattern formation: developing specific structures in specific locations

21. Plant Development Cell division = growth Determination = what cell can become Differentiation = cells become specific types Pattern formation Morphogenesis: organization into tissues & organs

22. Plant Development umbrella term for many processes embryogenesis

23. Plant Development umbrella term for many processes Embryogenesis Seed dormancy and germination

24. Plant Development umbrella term for many processes Embryogenesis Seed dormancy and germination Seedling Morphogenesis

25. Plant Development umbrella term for many processes Embryogenesis Seed dormancy and germination Seedling Morphogenesis Transition to flowering, fruit and seed formation

26. Plant Development umbrella term for many processes Embryogenesis Seed dormancy and germination Seedling Morphogenesis Transition to flowering, fruit and seed formation Many responses to environment

27. Plant Development Umbrella term for many processes Unique features of plant development Cell walls: cells can’t move: Must grow towards/away from signals

28. Plant Development Umbrella term for many processes Unique features of plant development Cell walls: cells can’t move: must grow instead Plasticity: plants develop in response to environment

29. Unique features of plant development Cell walls: cells can’t move Plasticity: plants develop in response to environment Totipotency: most plant cells can form an entire new plant given the correct signals

30. Unique features of plant development Cell walls: cells can’t move Plasticity: plants develop in response to environment Totipotency: most plant cells can form an entire new plant given the correct signals Meristems: plants have perpetually embryonic regions, and can form new ones

31. Unique features of plant development Cell walls: cells can’t move Plasticity: plants develop in response to environment Totipotency: most plant cells can form an entire new plant given the correct signals Meristems: plants have perpetually embryonic regions, and can form new ones No germ line!

32. Unique features of plant development Meristems: plants have perpetually embryonic regions, and can form new ones No germ line! Cells at apical meristem become flowers: allows Lamarckian evolution!

33. Unique features of plant development Meristems: plants have perpetually embryonic regions, and can form new ones No germ line! Cells at apical meristem become flowers: allows Lamarckian evolution! Different parts of the same 2000 year old tree have different DNA & form different gametes

34. Plant Cell Theory 1) All organisms are composed of one or more cells

35. Plant Cell Theory 1) All organisms are composed of one or more cells 2) Cell is smallest living organizational unit

36. Plant Cell Theory 1) All organisms are composed of one or more cells 2) Cell is smallest living organizational unit 3) Cells arise by division of preexisting cells

37. Plant Cells 1) Highly complex and organized

38. Plant Cells 1) Highly complex and organized 2) Metabolism

39. Plant Cells 1) Highly complex and organized 2) Metabolism 3) Reproduction

40. Plant Cells 1) Highly complex and organized 2) Metabolism 3) Reproduction 4) Heredity

41. Plant Cells 1) Highly complex and organized 2) Metabolism 3) Reproduction 4) Heredity 5) Mechanically active

42. Plant Cells 1) Highly complex and organized 2) Metabolism 3) Reproduction 4) Heredity 5) Mechanically active 6) Respond to stimuli

43. Plant Cells 1) Highly complex and organized 2) Metabolism 3) Reproduction 4) Heredity 5) Mechanically active 6) Respond to stimuli 7) Homeostasis

44. Plant Cells 1) Highly complex and organized 2) Metabolism 3) Reproduction 4) Heredity 5) Mechanically active 6) Respond to stimuli 7) Homeostasis 8) Very small

45. Why are cells so small? 1) many things move inside cells by diffusion

46. Why are cells so small? 1) many things move inside cells by diffusion 2)surface/volume ratio

47. Why are cells so small? 1) many things move inside cells by diffusion 2) surface/volume ratio surface area increases more slowly than volume

48. Why are cells so small? 1) many things move inside cells by diffusion 2) surface/volume ratio surface area increases more slowly than volume exchange occurs only at surface eventually have insufficient exchange for survival

49. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell

50. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell Protects & gives cell shape

51. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell Protects & gives cell shape 1˚ wall made first mainly cellulose

52. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell Protects & gives cell shape 1˚ wall made first mainly cellulose Can stretch!

53. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell Protects & gives cell shape 1˚ wall made first mainly cellulose Can stretch! 2˚ wall made after growth stops

54. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell Protects & gives cell shape 1˚ wall made first mainly cellulose Can stretch! 2˚ wall made after growth stops Lignins make it tough

55. Plant Cells 1)Cell walls Carbohydrate barrier surrounding cell Protects & gives cell shape 1˚ wall made first mainly cellulose Can stretch! 2˚ wall made after growth stops Lignins make it tough Problem for "cellulosic Ethanol" from whole plants

56. Plant Cells 1)Cell walls 1˚ wall made first 2˚ wall made after growth stops Lignins make it tough Problem for "cellulosic Ethanol" from whole plants Middle lamella = space between 2 cells

57. Plant Cells 1)Cell walls 1˚ wall made first 2˚ wall made after growth stops Middle lamella = space between 2 cells Plasmodesmata = gaps in walls that link cells

58. Plant Cells Plasmodesmata = gaps in walls that link cells Lined with plasma membrane

59. Plant Cells Plasmodesmata = gaps in walls that link cells Lined with plasma membrane Desmotubule joins ER of both cells

60. Plant Cells Plasmodesmata = gaps in walls that link cells Lined with plasma membrane Desmotubule joins ER of both cells Exclude objects > 1000 Dalton, yet viruses move through them!

61. Types of Organelles 1) Endomembrane System 2) Putative endosymbionts

62. Endomembrane system Common features derived from ER

63. Endomembrane system Common features derived from ER transport is in vesicles

64. Endomembrane system Common features derived from ER transport is in vesicles proteins & lipids are glycosylated

65. Endomembrane system Organelles derived from the ER 1) ER 2) Golgi 3) Vacuoles 4) Plasma Membrane 5) Nuclear Envelope 6) Endosome 7) Oleosomes

66. ER Network of membranes t/out cell 2 types: SER & RER

67. SER tubules that lack ribosomes fns: 1)Lipid syn 2)Steroid syn 3)drug detox 4)storing Ca 2+ 5)Glycogen catabolism

68. RER Flattened membranes studded with ribosomes 1˚ fn = protein synthesis -> ribosomes are making proteins

69. ER SER & RER make new membrane!

70. GOLGI COMPLEX Flattened stacks of membranes made from ER

71. GOLGI COMPLEX Individual, flattened stacks of membranes made from ER Fn: “post office”: collect ER products, process & deliver them Altered in each stack

72. GOLGI COMPLEX Individual, flattened stacks of membranes made from ER Fn: “post office”: collect ER products, process & deliver them Altered in each stack Makes most cell wall carbohydrates!

73. GOLGI COMPLEX Individual, flattened stacks of membranes made from ER Fn: “post office”: collect ER products, process & deliver them Altered in each stack Makes most cell wall carbohydrates! Protein’s address is built in

74. VACUOLES Derived from Golgi; Fns: 1)digestion a) Organelles b) food particles

75. VACUOLES Derived from Golgi; Fns: 1)digestion a) Organelles b) food particles 2) storage

76. VACUOLES Derived from Golgi; Fns: 1) digestion a) Organelles b) food particles 2) storage 3) turgor: push plasma membrane against cell wall

77. VACUOLES Vacuoles are subdivided: lytic vacuoles are distinct from storage vacuoles!

78. Endomembrane system Organelles derived from the ER 1) ER 2) Golgi 3) Vacuoles 4) Plasma Membrane Regulates transport in/out of cell

79. Endomembrane system Organelles derived from the ER 1) ER 2) Golgi 3) Vacuoles 4) Plasma Membrane Regulates transport in/out of cell Lipids form barrier Proteins transport objects & info

80. Endomembrane System 5) Nuclear envelope: regulates transport in/out of nucleus Continuous with ER

81. Endomembrane System 5) Nuclear envelope:regulates transport in/out of nucleus Continuous with ER Transport is only through nuclear pores

82. Endomembrane System 5) Nuclear envelope:regulates transport in/out of nucleus Continuous with ER Transport is only through nuclear pores Need correct signal & receptor for import

83. Endomembrane System 5) Nuclear envelope: regulates transport in/out of nucleus Continuous with ER Transport is only through nuclear pores Need correct signal & receptor for import new one for export

84. Endomembrane System Nucleus: spherical organelle bounded by 2 membranes and filled with chromatin = mix of DNA and protein

85. Endomembrane System Nucleus: spherical organelle bounded by 2 membranes and filled with chromatin fns = information storage & retrieval Ribosome assembly (in nucleolus)

86. Endomembrane System Endosomes: vesicles derived from Golgi or Plasma membrane Fn: sorting materials & recycling receptors

87. Endomembrane System Oleosomes: oil storage bodies derived from SER Surrounded by lipid monolayer!

88. Endomembrane System Oleosomes: oil storage bodies derived from SER Surrounded by lipid monolayer! filled with lipids: no internal hydrophobic effect!