1. Chapter 8 Photosynthesis

2. I./2. Some organisms are producers and others are consumers. a)Autotrophs a)Autotrophs make their own energy from inorganic sources through the process of photosynthesis or chemosynthesis a)Heterotrophs a)Heterotrophs are organisms that consume organic compounds in their food 9/12/20152

3. Why is Photosynthesis Important? Energy Flow through an ecosystem: Almost all of the biochemical energy on Earth comes directly or indirectly from the sun 9/12/20153

4. Photosynthesis Photosynthesis is the production of carbohydrates (glucose) from CO 2 and H 2 O, with the help of sunlight. 9/12/20154 6CO 2 + 6H 2 O --------------> C 6 H 12 O 6 + 6O 2 sunlight carbon dioxide waterglucoseoxygen Chemical energy out (sugar) Light energy in Inorganic molecules

5. Photosynthesis Location Takes place in the organelle called the Chloroplast Chloroplasts contain Chlorophyll, a pigment that captures light energy to make glucose

6. A Closer Look at Chloroplasts -Inside the chloroplasts are disk shaped sacs, called the thylakoid. - This is where the light dependent reactions occur -The thylakoids are arranged in stacks called Granum -The thylakoids are bathed in a thick fluid called stroma. This is where the calvin cycle takes place -Chlorophyll is the green pigment embedded in the thylakoid

8. Structure of the Leaf Chloroplasts are in the middle layer of leaves called the mesophyll

9. On the underside of the leaf, the stomata allows gases (CO 2 and O 2 ) to enter and exit the leaf Guard Cells regulate the stomata Veins called xylem and phloem carry water, glucose and nutrients

10. Light Photon = a discrete packet of light energy. Light is either absorbed, transmitted or reflected

11. Light Used in Photosynthesis Chlorophyll pigments can absorb only some wavelengths of the visible light from the electromagnetic spectrum. Chloroplasts absorb mainly blue-violet and red- orange lights. This absorbed light energy is converted to chemical energy. Green light is reflected and transmitted by green plants – hence, they appear green.

12. Paper Chromatography: method for separating and identifying mixtures Rf value: Distance pigment traveled Distance solvent traveled

13. Photosynthesis Overview 6CO 2 + 6H 2 O --------------> C 6 H 12 O 6 + 6O 2

14. 1.What is the color of this pigment? 2.What are the inputs/reactants of the light dependent reaction? 3. What are the products of the light dependent reaction?

16. Two Stages of Photosynthesis Photosynthesis occurs in two stages. – Light reactions – Calvin Cycle The two stages are linked together by NADPH and ATP.

17. 17 Stored Energy in Chemical Bonds Energy Released for Cellular Work (By breaking chemical bonds) - ATP and NADpH store energy in chemical bonds - Energy is released when bonds are broken

18. Light Reaction When: Dependent on light and occurs only during the day Where: thylakoid membrane of the chloroplast. Purpose: Create energy carriers for Calvin Cycle Inputs: Light Energy + H 2 0 Outputs: Oxygen +ATP+ NADPH Destination of outputs: – Oxygen released from stomata – ATP and NADPH fuel Calvin cycle

19. Calvin Cycle Outcome: Creates G3P which is used to make Sugars Takes place in the Stroma Inputs: C0 2, ATP, NADPH Outputs: Sugar, ADP+ P, NADP + Destination of outputs: – Sugar used in the plant – NADP+ and ADP are recycled to light dependent cycle

20. 8.2 Light Dependent Reactions

21. Light Reactions Involves: Step 1: chlorophyll uses solar energy to split H 2 O into H + O 2 Step 2: O 2 escapes as a waste product Step 3: Conversion of light into chemical energy Electrons and hydrogen are used to make NADPH, an energy rich molecule and ATP (chemical energy) Both ATP and NADPH store energy in their bonds They will be used in the Calvin Cycle (light independent reactions)

22. Photosystem II Photosystem I

23. Light Dependent Reaction (Photosystems I and II) 1.Clusters of pigments are embedded in the thylakoids. (Photosystems I and II) 2.Energy from the sun causes electrons in the pigments to become “excited” jump to a higher energy level (Photosystem II loses an electron) 3. Photosystem II then “steals” an electron from H2O by splitting the molecule into H+H+O

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25. 9/12/201525 Oxygen production O 2 4. The Oxygen from two water molecules fuse to form O 2 gas, which is released as waste into the air. 5. Hydrogen molecules are released as free protons (H + )

26. 6. Electrons travel down the an electron transport chain. This process pumps H+ ions across the membrane into the thylakoid (The electron Transport chain) - this creates a high concentration of H+ inside the thylakoid

27. The Electron Transport Chain produces energy storage Compound ATP and NADPH NADPH 7. Excited electrons from Photosystem I travel through an ETC and help produce NADPH. a)H + is added to NADP + to create NADPH (stored energy) b)These electron will be replaced by electrons from Photosystem II.

28. enzyme ATP synthase 8. H + floods back out through an enzyme channel called ATP synthase. 9. Each H + one ATP 9. Each H + that is transported aids in the production of one ATP molecule. 10. NADPH and ATP will be used to fuel the Calvin Cycle in the Stroma 9/12/201528

30. 9/12/201530 How are the following reactants used in the light dependent stage of photosynthesis? - Sunlight - Water How are the following products created during the process of photosynthesis? - Oxygen

31. http://highered.mcgraw- hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/007243 7316/120072/bio13.swf::Photosynthetic%20Electron%20Transport%20an d%20ATP%20Synthesis

32. Light Independent Reaction: Calvin Cycle Overview: use CO2 and Energy rich molecules (ATP and NADPH) to create sugar

33. Light Dependent Reaction/ Calvin Cycle independent of light and occurs during the day and the night: Relies on light dependent products Where: stroma of the chloroplast. IN: ATP, NADPH, CO2 OUT: G3P which will be used to make glucose, ADP + P, NADP+(Both will be energized again in light dependent reactions) Calvin Cycle involves the production of ‘food’ as glucose, by using CO 2, ATP energy and NADPH.

34. 6H 2 06CO 2 LightC 6 H 12 O 6 6 O 2 In what reaction is this used/ made Describe Specifically how this is used/ made

36. Calvin Cycle Steps: 1. CO2 enters the stroma 2. It combines with a 5-C sugar RuBP using the enzyme Rubisco to form a 3-C acid (carbon fixation) 3.Using energy from ATP & NADPH high energy sugars (G3P) are made for the plant - ATP donates a phosphate group and NADPH donates electrons and Hydrogen molecules 4. ADP & NADP + go back to the thylakoid to be “recharged”

37. BIG PICTURE: -In the end, 6 carbon dioxide molecules enter the cycle to make one six-carbon glucose. -The energy required to do this came from the NADPH and ATP that was formed with the help of the sun’s energy.

38. Fate of Atoms in Photosynthesis CO 2  becomes part of glucose (C 6 H 12 O 6 ) H 2 O  is split into H and O; O 2 is released as a gas; H’s become part of glucose.