2. RECOMBINANT DNA TECHNOLOGY PART 2

3. Topics Genomic libraries Id a specific clone or sequence within a library Transgenic plants Transgenic animals Timely topics

4. Genomic library contains all of the organism’s genome Probe design

5. Bacterial clones and finding desired clone Probe design

6. A Final about vectors : There are many types Small vectors Large vectors Expression vectors Viral vectors

7. Transgenic Mice

8. transgene Linear DNA Non-homologous recombination

9. Knockout Mice

10. Phenotype of the knockout mouse gives information on protein function Homozygous knockout G418 gancyclovir

11. Applications of DNA technology Agriculture Drug design Genetic testing Gene therapy

12. Genetic Modified Plants: “GM” Tobacco with luciferase gene from firefly Transgenic plant

13. Tumor Inducing Plasmid

14. Model for making genetically modified (GM) plants

15. GM crops Bt toxin genes (Bacillus thuringensis)

16. Bt Toxin Cry toxins and cry genes: damages gut of larvae

17. GM Plants Roundup  ready plants: herbicide resistant Herbicide known as: Glyphosate

18. Glyphosate Enzyme inhibitor Prevents amino acid synthesis Prevents weed growth Environmental concerns

19. Antisense Technology 5´ C A U G 3´ mRNA 3´ G U A C 5´ Antisense RNA

20. Anti-Sense Technology & FLAVR SAVR TM Tomato Anti-sense DNA technology inhibits polygalacturonase (PG)

21. The Flavr-Savr Tomato Ripens on the vine Preserves firmness and flavor Uses anti-sense technology

22. Cloning of a whole organism: Reproductive Cloning

23. Somatic CellGerm Cell For ex. Mammary cellovum SHEEP CELLS 54 27

24. Somatic CellGerm Cell For ex. Mammary cellovum How to process begins: 54 27 54 discarded

25. Now the germ cell contains 54 chromosomes! The germ is stimulated “electrically”. Cell division in some instances begins. Individual is identical to host “DNA” donor. Aging affects? “Was Dolly a sheep in lamb’s clothing?”

26. Somatic Gene Therapy

27. Successes SCIDS (Severe Combined Immunodeficiency Disease) Head and neck cancers

28. But also failures and controversy