1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 27, part C Environmental Microbiology

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Pollutants (nutrients) may cause algal blooms. Algal blooms lead to eutrophication. Algal Blooms Figure 27.15

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Aerobic or facultatively anaerobic, gram-negative, non–endospore forming rods that ferment lactose to acid + gas within 48 hr, at 35°C Indicator organisms Used to detect fecal contamination MPN Most probable number/100 ml of water Coliforms

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Multiple-Tube Method Figure 6.18a

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Multiple-Tube Method Figure 6.18b

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings ONPG causes E. coli to make  -galactosidase MUG Test Figure 27.16 MUGfluorescent compound  - galactosidase

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Municipal Water Treatment Figure 27.17

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Municipal Sewage Treatment Figure 27.19

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Activated Sludge Figure 27.20a,b

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings CO 2 + 4 H 2  CH 4 + 2 H 2 O CH 3 COOH  CH 4 + CO 2 Anaerobic Sludge Digester Figure 27.23