1. Stuart D. Blacksell MPH PhD RBP Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. Centre for Tropical Medicine, University of Oxford, United Kingdom. Stuart D. Blacksell MPH PhD RBP Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. Centre for Tropical Medicine, University of Oxford, United Kingdom. Design and operational options for improving sustainability, biosafety, and biosecurity in southeast Asia.

2. Local Challenges: building a BSL3 lab

3. Local Challenges: building a BSL3 lab Cont.

4. Local Challenges: Biosecurity engineers

5. Common design mistakes

6. Local Challenges: Other BSL3 engineering “issues ”

7. Facilities maintenance and testing

8. Other considerations BSL3 Energy costs –Tropical climate –BSL3 single pass air = very expensive and wasteful –Why not recirculation 85% of the air with additional HEPA filtration Regional training faculties –Biocontainment engineering/Biosafety Officers/Maintenance –Not for profit –Use facilities and experience that is already available locally Assess the risks –Do we really need a BSL3 lab? –Can we use BSL2 lab with BSL3 practices

9. Energy conservation? (some thoughts) Now…Future??... AHU HEPA EF BSL3 LAB BSC

10. Conclusions Focus areas for building a BSL3 lab –Standards, regulations and laws Know what standard (BMBL5?) –People Project manager (prequalify) Biocontainment engineer (prequalify) General contractors (prequalify) Client (be knowledgeable) –Documents Specifications compliance Budget and contingencies

11. Thank you