Presentation on theme: "University of Health Sciences Proposition of monograph of Terminalia nigrovenulosa Pierre ex. Prepared by: Channeth Meng 1, Sothea-Kim 1-2, Philippe-Bessioud."— Presentation transcript:
University of Health Sciences Proposition of monograph of Terminalia nigrovenulosa Pierre ex. Prepared by: Channeth Meng 1, Sothea-Kim 1-2, Philippe-Bessioud 2-3, Davy Theng, Chhommonica Pech 2, Mathieu Leti 2-3, Bernard Fabre 2-3.
University of Health Sciences Outline I.Introduction II.Project’s purpose III.Research methodology IV.Result V.Conclusion
University of Health Sciences I.Introduction: Traditional medicine (TM) still plays an important role in healthcare system in Cambodia, especially in rural area. There are many types of medicinal plants that are used. Among those Terminalia nigrovenulosa is frequently used as anti-diarrhea and dysentery. Terminalia nigrovenulosa’s stem bark is used in treatment of aphtha, generally in Asian country (weerapeeyakul, 2012).
University of Health Sciences II.Project’s purpose : To propose the monograph of Terminalia nigrovenulosa in Cambodian pharmacopeia.
University of Health Sciences III.Research methodology: 1.Plant collection 2.Microscopic study 3.Colored reaction 4.Thin Layer chromatographic (TLC) Test 5.Determine the quantity of total polyphenol
University of Health Sciences 1.Plant collection 10 samples testing 2.Microscopic study Identify the micro-element In powder stem’s bark
University of Health Sciences 3.Colored reaction Identify the tannin: 5ml extract + 1ml solution FeCl3 Black color/green black Identify the tannin catechic: 10ml extract + 5ml stiasny Precipitation (2) Identify tannin gallic: Filtrate (2) + C 2 H 3 00Na + 1ml FeCl 3 Blue-black precipitation
University of Health Sciences 4.TLC Test: Control reagent: Gallic acid and Rutin Mobile phase: Water/ Formic acid/ Ethyl acetate (8/8/80) Revealer : blue salt solid B
University of Health Sciences 5.Determine the quantity of total polyphenol UV/Visible Spectrophotometry Control reagent : gallic acid Stem bark extract : Ethanol-water 50% extract
University of Health Sciences 2.Identify the micro-element of stem bark: Type of micro-elementPhoto of fragments Fragment of parenchyma 10x Sclerotic cells of the outer seed coat assie 10x Calcium oxalate cristal 10x
University of Health Sciences 2.Identify the micro-element of stem bark: Fiber bundle Associates with liberoligneur 10x Mesocarp sclerotic cells 10x Debris suber in yellow colored walls 10x
University of Health Sciences 3.Colored reaction: Reaction T. chebula (CL 1411) T. catapa (CL1482) T. nigrovenulosa (CL1486) T. nigrovenulosa (MPM302) Colored reaction Tannins ++++ Tannins catechic ++++ Tannins gallic ++++
University of Health Sciences 4.TLC Test: Top of plat Gallic acid : red band (Rf ~ 0,90) Rutin : yellow band (Rf ~ 0,16) 1 Red band low intensity (Rf ~ 0,65) 2 Red band(Rf ~ 0,5, 0,46) 2 Red band with low intensity (Rf ~ 0,27, 0,17) One red band (Rf ~ 0.11) Control reagent Stem bark extract
University of Health Sciences 5.Determine the quantity of total polyphenol: Plant codeAverage concentration (%)SDVariance(%) CL144814.750.503.42 CL147016.480.382.29 CL148614.980.110.74 CL149212.380.252.02 MPM28413.381.047.77 MPM28614.740.453.06 MPM28812.410.403.20 MPM28913.560.634.64 MPM29115.290.593.84 MPM30215.280.241.58
University of Health Sciences 5.Determine the quantity of total polyphenol
University of Health Sciences V.Conclusion The simple quality controls’ methods of Terminalia nigrovenulosa are developed and proposed.