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Intestinal` Protozoa. Giardia lamblia Giardia trophozoites ( SEM) Giardia trophozoites (light microscope)

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Presentation on theme: "Intestinal` Protozoa. Giardia lamblia Giardia trophozoites ( SEM) Giardia trophozoites (light microscope)"— Presentation transcript:

1 Intestinal` Protozoa

2 Giardia lamblia Giardia trophozoites ( SEM) Giardia trophozoites (light microscope)

3 Giardia trophozoites (light microscope)

4 Giardia cyst (light microscope)

5 Giardia trophozoites (light microscope) Trichrome stain Unstained

6 Giardia lamblia : Life cycle

7 Unstained Trichrome stain Giardia cyst (light microscope)

8 Giardia trophozoites in tissue section

9 Giardia cyst and trophozoites IH stain

10 Giadriasis: Clinical Picture Asymptomatic infections ( majority) Symptomatic Infections: Typical picture: IP 1-2 wks followed by diarrhoea for about 6 wks, Atypical : Severe diarrhoea, malabsorption especially in children

11 Giardiasis: Laboratory diagnosis Stools examination :cysts or trophozoits Examination of duodenal contents : trophozoites

12 Giardiasis: Chemotherapy Drug of choice: Metronidazole

13 Intestinal Amoebae

14 Entamoeba coli

15 Iodamoeba butschlii trophozoite

16 Iodamoeba butschlii cyst

17 Endolimax nana cyst

18 Endolimax nana trophozoite Endolimax nana cyst

19 E. polecki cyst E. polecki trophozoite

20 Entamoeba coli (drawing)Entamoeba coli (Eosin)

21 Entamoeba coli ( cyst )

22 Entamoeba coli ( trophozoite)

23 Entamoeba hartmanni

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25 Entamoeba histolytica

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29 E. histolytica

30 Entamoeba histolytica

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32 ENTAMOEBA HISTOLYTICA… 500 million people are infected. 100,000 deaths per year. Worldwide distribution. It is a waterborne infection. There are 6 species of Entamoeba: histolytica dispar hartmanni coli gingivalis polecki

33 Entamoeba histolytica : amoebae that are pathogenic. E. dispar The non invasive form. The 2 amoebae can ’ t be distinguish by microscopic observation. E. histolytica vs E. dispar

34 Mode of infection Water, food Flies can act as vector.. Can be sexually transmitted person -to - person contacts Not a zoonosis Entamoeba histolytica

35 Life cycle Trophozoite: vegetative stage, must encyst to survive in the environment. It is a fragile structure. Pre cyst: encysting trophozoites disgorging any undigested food. Cyst: infective stage. Resist to the harsh conditions of the environment. Entamoeba histolytica

36 The infective dose can be as little as 1 cyst. be as little as 1 cyst. The incubation period can be from few days to can be from few days to few weeks depending on the infective dose Cysts can survive for weeks at appropriate weeks at appropriate temperature and humidity. Entamoeba histolytica

37 PATHOLOGY Intsetinal amoebiasis : Remarkable and unique ability a to hydrolyse host host tissues with their active cysteine proteases present on the surface membrane of the trophozoite. Lesions are found in the caecum, appendix, or colon. They may heal. If perforation of the colon occurs, My lead to a peritonitis that can lead to death. Amoeboma Granuloma obstructing the bowel Entamoeba histolytica

38 PATHOLOGY: Intsetinal amoebiasis :

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40 PATHOLOGY : Intsetinal amoebiasis Complications

41 Entamoeba histolytica PATHOLOGY Intsetinal amoebiasis :

42 Entamoeba histolytica PATHOLOGY Intsetinal amoebiasis :

43 E. Histolytica in mucosa. in mucosa. Numerous trophozoites can be seen with ingested seen with ingested erythrocytes. PATHOLOGY Intsetinal amoebiasis :

44 PATHOLOGY: Extra-intsetinal amoebiasis :

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47 Free-living Amoebae

48 Acanthamoeba in corneal scraping

49 Acanthamoeba trophozoite

50 Acanthamoeba brain

51 Acanthamoeba

52 Acanthamoeba ulcer

53 Naegleria brain

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59 Cryptosporidium oocysts

60 Cryptosporidium, safranin Cryptosporidium, acid-fast stain

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63 Cryptosporidium

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