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Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli.

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Presentation on theme: "Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli."— Presentation transcript:

1 Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli

2 MacConkey Agar Left: Shigella - result Right: Escherichia + result

3 Carbohydrate Fermentation Tests Glucose w/ Durham Fermentation Tube

4 Carbohydrate Fermentation Tests Sucrose Fermentation

5 Triple Sugar Iron -/+/+

6 Indole Test broth with tryptophan IMViC Series

7 Methyl Red Test IMViC Series

8 Voges-Proskauer Test  Leave uncapped for minutes…….. IMViC Series MRVP broth

9 Citrate Utilization Test - + IMViC Series

10 Urease Test Slow positive Negative result (acidic) Uninoculated urea agar Positive result (alkaline)

11 Motility Test

12 MacConkey agar 1. Which plate could have E. coli on it? 2. Which plate shows the presence of a lactose-fermenter? 3. Which plate could have Salmonella on it? 4. Which plate shows the presence of a lactose non-fermenter? Answers: 1.A3. B 2. A4. B A B

13 What is the name of the lab procedure demonstrated here? Answer: Stabbing the butt!

14 Use the chart on p. 181 and or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C U Answer: Shigella sonnei

15 I M V C U Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! Answer: Escherichia coli

16 I M V C U Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! Answer: Serratia marcesens

17 Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C Answer: Klebsiella pneumoniae

18 Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C U Answer: Salmonella typhimurium

19 Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C U Answer: Proteus vulgaris

20 Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C Answer: Enterobacter aerogenes U


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