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Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase
Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink
Gram Reaction Morphology O2Ferment Lactose Urease E. coli (-) Bacilli Facultative (+) (-) Proteus vulgaris (-) Bacilli Facultative (-) (+) Salmonella (-) Bacilli Facultative (-) Shigella (-) Bacilli Facultative (-)
Gelatinase Gelatin (solid) Gelatin (liquid) Tryptic Soy Gelatin Agar
Nitrate Reductase Nitrate Nitrite + water Facultative Bacteria Nitrate Broth
PHT 416 Lab 8. Steps Microscopic Morphology Growth Biochemical Tests Nutrient agar Blood agar Mannitol Salt Agar MacConkey’s agar.
Nitrate reduction test. 3 possibilities Nitrate – nitrate reductase – nitrite Nitrate – nitrate reductase – nitrite – Nitrite reductase – nitrogen gas.
Lab. No. 5. Gram-negative, non-spore-forming bacilli. Gram-negative, non-spore-forming bacilli. Their natural habitat is the intestinal tract of humans.
Urease test objective To differentiate between urease positive and urease negative bacteria using Christensen urea agar. principle Some bacteria can.
Carbohydrates 1. Monosaccharides glucose, fructose, galactose 2. Disaccharides sucrose, maltose, lactose 3. Polysaccharides starch, glycogen, Agar.
Lab. No. 7. II. Enterobacteriaceae It divided into two main groups: It divided into two main groups: According to their effect on lactose Lactose.
General Microbiology Laboratory Biochemical Tests.
Some bacteria are able to produce an enzyme called urease that attacks the nitrogen and carbon bond in amide compounds such as urea, forming the end.
Summary of Biochemical Tests in Microbiology Better living through chemistry.
BIOCHEMICAL TESTING. Distinguishing Enterobacteriaceae Many found in the intestines of human or other mammals Varrying pathogenicity: Commensals, opportunists.
Enterobacteriaceae II - Microscopic appearance - Cultural characteristics - Biochemical Tests of Enterobacteriaceae (Non- Lactose fermenters). - Identification.
General Microbiology Laboratory Biochemical tests.
Biochemical Activities of Bacteria Bacteria accomplish their various biochemical activities (growth and multiplication) using raw materials (nutrients)
Gram-negative rods Enterobacteriacea Clinical Microbiology MESHACK JUMA MEDICAL MICROBIOLOGY DEPT (UON) Msc. Med Micro (UON), PGD-RM(UON), Bsc. Med Micro(JKUAT),
University of Tabuk Faculty of Applied Medical Science Department of Medical Laboratory Technology Mr.AYMAN.S.YOUSIF M.SC IN Microbiology &IMMUNOLOGY Academic.
CULTURE MEDIA LECTURE 5: Microbiology and Virology; 3 Credit hours Atta-ur-Rahman School of Applied Biosciences (ASAB) National University of Sciences.
Physiological characteristics: Oxidative and fermentation tests Lab # 8 Medgar Evers College Prof. Santos.
Biochemical Activities of Microorganisms Part (2).
Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli.
Lab 11 Goals and Objectives: Exercise 39: Oxidation and Fermentation Tests Read results: some tubes will require additional reagents Do controls first.
Week 8 W Exp 22: Carbohydrate Fermentation Lab Two pg 158 Exp 23: Triple Sugar Iron Agar Test Lab Two pg 162 Charts New: Exp 24: IMViC Part A: Indole Test.
General Microbiology Laboratory By: Mahmoud W El-Hindi1.
Enterobacteriaceae - Microscopic appearance - Cultural characteristics - Biochemical Tests of Enterobacteriaceae - Identification of Enterobacteriaceae.
Lab 12 Goals and Objectives: Exercise 40: Hydrolytic and Degradative Reactions Read results: some will require additional reagents Exercise 41: Multiple.
Metabolic Diversity Two fundamental nutritional needs: 1.Acquiring chemical energy in the form of ATP – Pump ions, move cargo, undergo chemical reactions.
Lab 11 Goals and Objectives: Exercise 39: Oxidation and Fermentation Tests Read results: some tubes will require additional reagents (share reagents across.
Gram-negative rods Enterobacteriaceae. Characters of Enterobacteriaceae All Enterobacteriaciae Enterobacteriaciae –Gram-negative rods –Ferment glucose.
Biochemical Tests Mohammed Laqqan Enterobacteriaceae Classification – more than15 different genera Escherichia Shigella Salmonella Citrobacter Klebsiella.
CULTURE MEDIA LECTURE 7: Microbiology and Virology; 3 Credit hours Atta-ur-Rahman School of Applied Biosciences (ASAB) National University of Sciences.
Ex. 16: Carbohydrate Fermentation Objectives??. Fermentation Tubes Contain Peptones and NaCl COH of interest (0.5 – 1%) Phenol red indicator ( alternate.
Identifying Bacteria based on Enzymes and multiple test media Lab # 9 Medgar Evers College Prof. Victor Santos.
Lab 8&9 Goals and Objectives : Exercise 37: Morphological Study of Unknown Bacterium Exercise 38: Cultural Characteristics Discuss observations of the.
Practical 7 Biochemical properties testing 1 part.
Mic 224 Lab 10 IMViCs. IMViC Tests The IMViC tests are useful for differentiating the Enterobacteriaceae, especially when used alongside the urease test.
313 PHT Lab. No. 8. Aerobic, non-fermentative, motile, oxidase-positive gram- negative bacilli. Aerobic, non-fermentative, motile, oxidase-positive.
General Microbiology Laboratory 1. By: Mahmoud W El-Hindi2.
Enzyme lab. Introduction In this experiment we tried to look how pH can control the color of indicator. We also looked at the effect of urease from soya.
Non-Fermentative Gram-Negative Rods Pseudomonas spp.
Introduction to Lab Ex. 16 Diagnostic Media Urea broth Sulfide Indole Motility Citrate.
Biochemistry I Labs 22,23,24. Exoenzymes – Lab 22 Bacteria make enzymes to digest macromolecules outside of the cell The smaller sub units of the enzymatic.
MICROBIOLOGY LAB TESTS. The following microbiology tests will be performed in lab this semester Gram stain Motility EMB ( Eosin Methylene blue agar)
Introduction to Biochemical tests. In the microbiology lab, biochemical test relays on enzymes which is glycoprotein or protein that act as catalyst by.
IDENTIFICATION OF ENTEROBACTERIACEAE Prepared by: Abdullah Ali Supervised by: Dr.Abdellraof el-Manama Dr.Nedal Fayyad الجامعة الاس مية بغزه كلية العلوم.
Lab #8. Review of Lab #7 - pH Indicators pH Indicator Very acidic AcidicNeutralBasic Phenol red- pH <6.8 = yellow pH = red pH >8.0 = magenta/
Differential and Selective Media Prepared BY Ms.c: Abed Al Rahman I. Hamad.
Biochemistry Class Nine. Macromolecules Microorganisms and their Identification Determination of pathogens responsible for infectious diseases Selection.
Amino Acid Enzymes Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase.
Gram Positive Staphylococci – (Note the purple, spherically- shaped, clustered cells)
Microbiology Gram-negative rods: Enterobacteriaceae Part I Karen Honeycutt, M.Ed., MT(ASCP)SM CLS 418 Clinical Microbiology I Student Laboratory Session.
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