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Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase
Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink
Gram Reaction Morphology O2Ferment Lactose Urease E. coli (-) Bacilli Facultative (+) (-) Proteus vulgaris (-) Bacilli Facultative (-) (+) Salmonella (-) Bacilli Facultative (-) Shigella (-) Bacilli Facultative (-)
Gelatinase Gelatin (solid) Gelatin (liquid) Tryptic Soy Gelatin Agar
Nitrate Reductase Nitrate Nitrite + water Facultative Bacteria Nitrate Broth
Shigella spp Isolation and Serotyping. Shigella spp Shigella is a group of bacteria that has four different species that cause gastrointestinal illness.
EMB agar Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based.
TOPIC: Ecology AIM: How are materials cycled through the environment?
Changes in Matter _________ Changes – identity of substance does _____ change Examples: _____________ _____________ __________ __________ _________ _________.
Microbiological Media Terms and Concepts. Forms of culture media All examples here allow air to enter without dust getting in (tortuous path) Agar is.
Calculate the number of moles. MM= 342 amu. Calculate the number of moles. FM= 58.5 amu mol sugar.
INDICATORS & pH: Part 2 What is the difference between an acid and a base? Acid: H 3 O + (hydronium) ion Base: OH - (hydroxyl) ion Acids: react with metals.
Word Equations- State of the substance The reactants are the names of the substances that you begin with at the start of the reaction and the products.
Objectives Be able to define what an indicator microorganism is and why they are used Be able to list the criteria for an ideal indicator organism Be able.
Microbiology Methods and Applications NJDEP Office of Quality Assurance
PENDAHULUAN SISTEM PENGOLAHAN AIR, UDARA & LIMBAH PADAT.
Liquid Excretion: removal of liquid wastes & water/salt balance Ch 37.3 The Urinary System.
BW- Paper Lab BACKGROUND: In water, carbon dioxide dissolves to form a weak acid. As a result, an acid-based indicator such as bromothymol blue can be.
PH. What is pH? pH is a measure of how acidic or basic a solution is pH of a solution is compared to the pH scale.
Sulfite 2- (Sulfite) (SO 3 ) 2- Often listed in ingredients as sulfur dioxide (SO 2 ); however, in contact with water this becomes sulfurous acid (H 2.
Dehydration Synthesis (Condensation Reaction) Dehydration Synthesis (Condensation Reaction) Built Up Removing Water monomers Polymers Glucose + fructose.
Nitrogen Cycle Ch.2.2 Bacteria Decomposers in the Nitrogen Cycle.
Why Study Water Surface – Dirt Minerals Organic materials (any once living thing now decomposed Fertilizer Chemicals Anything spilled Ground – aquifers.
TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST TEST.
Measure out between 0.45 and 0.55 gram of sodium carbonate and react with enough acid to totally consume the carbonate.
Cycling of Materials in Ecosystems Cycles within an Ecosystem Water Cycle Carbon Cycle Phosphorous and Nitrogen Cycle.
USE OF A UNIQUE BIOBARRIER TO REMEDIATE NITRATE AND PERCHLORATE IN GROUNDWATER Presented by Betty A. Strietelmeier Los Alamos National laboratory To 2001.
Units for Measuring Water Quality PARTS PER MILLION Most dissolved substances found in water are measured in parts per million (ppm) or even smaller.
Lab Two, Part 2: Blood and MSA, pg 429 Lab Two, Part 1: Coagulase Test, pg 429 Exp 29: Catalase Test Physical Factors Exp 15: Temperature Exp 16: pH Exp.
I. Domain: Bacteria A. Taxonomic Classification 1. Bacteria are classified according to rRNA similarities morphological similarities biochemical similarities.
Ch 11 Acids & Bases Test Review. 1.Color Change; Universal indicator- yellow, orange and red Blue Litmus paper turns red 2.) Sour taste and conducts.
1 Chapter 11 Chemical Reactions. 2 Section 11.1 Describing Chemical Reactions Objective 1: You will learn to identify when a chemical reaction takes place.
Water Contaminants. Nitrate Acceptable ranges: Source of contaminant: Indication of contaminant: Health Problems: Solution: 0-10 mg/L Human and animal.
Certain types of bacteria indicate animal and human waste pollution. Escherichia coli are coliform bacteria found in the intestines of warm-blooded organisms.
Option F: Microbes and Biotechnology F.2 Microbes and the Environment.
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