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BIOCHEMICAL UNKNOWN LAB REPORTS Name_______________________ Unknown # ________ Take an unknown and write down its number. Inoculate 2 nutrient agar slants.

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Presentation on theme: "BIOCHEMICAL UNKNOWN LAB REPORTS Name_______________________ Unknown # ________ Take an unknown and write down its number. Inoculate 2 nutrient agar slants."— Presentation transcript:

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2 BIOCHEMICAL UNKNOWN LAB REPORTS Name_______________________ Unknown # ________ Take an unknown and write down its number. Inoculate 2 nutrient agar slants. Inoculate one agar plate w/ the streak method of isolation of colonies. Study the unknown following this format: Flow Chart: ………………………………………………………………………5Pts Staining: ………………………………………………………………………….9Pts 1. Gram Stain: (+)/(-): _________ 2. Endospore stain: (Spores present/ Spores not present) ___________ 3.Acid fast stain( Acid fast/ Non acid Fast): _______________________ Cellular characteristics: ………………………………………………………..6Pts 1.Cellular shape: Cocci/ bacilli/ Pleomorphic: ______________________ 2.Arrangement: Strep (chains)/ Staph (bunch)/ single/ pairs/ tetrads/ other: _____________________________________________________________ _____________________________________________________________ 3. Motility(Motile/ Non motile): ______________________ Note: Use the Hanging Drop Method) Cultural characteristics: ………………………………………………………10Pts 1.Size: ____________________________________________________________ 2.Pigmentation: _____________________________________________________ 3.Form: ____________________________________________________________ 4.Margins: __________________________________________________________ 5.Elevation: _________________________________________________________

3 Cont. BIOCHEMICAL UNKNOWN LAB REPORTS Biochemical tests: ……………………………………………………………30Pts 1.Catalase: _____________ 2.Oxidase:______________ 3.Indol: ________________ 4.MR: _________________ 5.VP: __________________ 6.Citrate: _______________ 7. Urease: ______________ 8.Phenyl Alanine deaminase: _______________ 9.Lysine decarboxylase: ___________________ 10.Ornithine decarboxylase: _________________ 11.Gelatinase: ____________________________ 12.H 2 S production: _________________________ 13.Nitrate Reductase: _______________________ 14.Starch: ______________________________ 15.Glucose fermentation: __________________ 16.Lactose fermentation: __________________ 17.Sucrose fermentation: __________________ 18.Gas production: ________________________ 19.Mannitol fermentation: __________________ 20.O/F: __________________________________ Brief literature review about the unknown: ………………………10Pts Unknown # _____ is: ______________________ Total.………..70Pts

4 Aeromonas hydrophila, Alcaligenes faecalis, Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, E. coli, Klebsiella pneumoniae, Neisseria sicca, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens, Shigella flexnerii

5 Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Enterococcus faecalis, Lactobacillus lactis, Micrococcus luteus, Micrococcus roseus, Mycobacterium phlei, Sporosarcina ureae, Staph aureus, Staph epidermidis, Strep pyogenes, Strep salivarius

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7 26 26. Strep. salivarius: (-) 23 Staph aureus: (+) 15 Micrococcus luteus : (+) Strep salivarius

8 Oxidase Test: a.To test for the presence of Cytochrome C Oxidase b.To differentiate Neisseria :Cytochrome C oxidase (+) c.To differentiate between E. coli (Cytochrome C Oxidase (-) from Pseudomonas (Cytochrome C Oxidase (+) d.Take two slides e.Put on top of each slide a piece(2 cm x 2cm) of bibulous paper. f.With a cotton swab roll a heavy inoculum of E.coli on one slide and Pseudomonas on the other slide g.Add 2 drops drops of the chromogenic reagent h.Wait for color change Chrom. Rgnt Red Chrom. Rgnt Ox Cytochrome bo/ bd Chrom. Rgnt Red

9 OXIDASE TEST After 5 min of adding the Chromogenic reagent After 13 min of adding the Chromogenic reagent Courtesy of Rieko Okawa


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