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MLAB 1415: Hematology Micros 60. 3-part differential Sample size= 10µL Environment – Indoor only – Level work station – Avoid direct exposure to sunlight.

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Presentation on theme: "MLAB 1415: Hematology Micros 60. 3-part differential Sample size= 10µL Environment – Indoor only – Level work station – Avoid direct exposure to sunlight."— Presentation transcript:

1 MLAB 1415: Hematology Micros 60

2 3-part differential Sample size= 10µL Environment – Indoor only – Level work station – Avoid direct exposure to sunlight – Proper ventilation – o F Linearity (AMR) – WBC (10 3 /mm 3 ): – RBC (10 6 /mm 3 ): – Hgb (g/dL): – Hct (%): – Plt (10 3 /mm 3 ):

3 Parameters WBC RBC Hgb Hct MCV MCH MCHC RDW Platelet MPV Lym # Lym% Mono # Mono% Gra# Gra% Measurements/Calculations WBC/RBC/PLT – Impedance change HGB – Spectrophotometry LYM%/MON%/GRA% – Impedance change  Calculations MCV, MCH,MCHC,RDW,MPV,LYM#, MON#, GRA#

4 Stats Principle of operation: The counting principle used for the red blood cells, white blood cells and platelets is based on the variations in impedance generated by the passage of cells through a calibrated aperture. Number of samples which can be analyzed per hour: 60 Number of tests performed on this analyzer: 1 Quality control: Three levels run each day of patient testing, or following instrument repair, reagent lot change, or when indicated. Calibration: Shift in control values, replacement of instrument parts, reagent number change, or when indicated Preventative maintenance: Daily: start up and shut down, general cleaning Acceptable samples: whole blood from an EDTA tube ran within 4 hours of collection Limitations: Lipemia, cold agglutinins, high WBC’s

5 Equipement 8 Main parts – Electrical supply – Electronic board – Dilution pneumatics – Control panel with keyboard and LCD screen – Reagent compartment – Printer – Smart card reader – Bar code reader (optional)

6 Reagents – Diluent ABX minidil: sodium hydroxide/azide Determines and differenciates blood cells – Cleaner ABX miniclean: enzymatic detergent Cleans system – LYSE: potassium cyanide ABX Alphalyse: Lyses RBCs, releasing hemoglobin and WBCs. WBCs are counted by impedance. Waste

7 Distribution curves WBC RBC Plt

8 Daily Start Up 1.Turn on instrument, once on, the start-up will begin automatically 1.Instrument is performing a cleaning and rinsing cycle: detergent in the chamber is rinsed with diluent 2.Cycle lasts about 130 seconds 2.At cycle completion, background values will be displayed. These values must be less than the below: 1.WBC < 0.3 x 10 3 /mm 3 2.RBC < 0.02 x 10 6 /mm 3 3.Hgb < 0.0 g/dL 4.Plt < 10x 10 3 / mm 3 5.If the values are not within this range, another cycle will begin. If still not in range, the Micros will do another start-up. After 3 failed cycles, it will give a “start-up failed” message. Troubleshooting will now start.

9 Daily start-up con’t 3.Run a primer sample 4.Perform QC and evaluate 5.Complete maintenance sheet 6.If all QC in, can continue with patient samples.

10 Running a Patient 1.Press the ID button on the key pad 2.Manually enter the ID. Use the up and down arrows to enter alphabetical characters. Please Enter after each entry.

11 Shutdown 1.Hit the Stand by key, wait 1 minute 2.Switch off instrument and printer.


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