4 StatsPrinciple of operation: The counting principle used for the red blood cells, white blood cells and platelets is based on the variations in impedance generated by the passage of cells through a calibrated aperture.Number of samples which can be analyzed per hour: 60Number of tests performed on this analyzer: 1Quality control: Three levels run each day of patient testing, or following instrument repair, reagent lot change, or when indicated.Calibration: Shift in control values, replacement of instrument parts, reagent number change, or when indicatedPreventative maintenance: Daily: start up and shut down, general cleaningAcceptable samples: whole blood from an EDTA tube ran within 4 hours of collectionLimitations: Lipemia, cold agglutinins, high WBC’s
5 Equipement 8 Main parts Electrical supply Electronic board Dilution pneumaticsControl panel with keyboard and LCD screenReagent compartmentPrinterSmart card readerBar code reader (optional)
6 Reagents Waste Diluent Cleaner LYSE: potassium cyanide ABX minidil: sodium hydroxide/azideDetermines and differenciates blood cellsCleanerABX miniclean: enzymatic detergentCleans systemLYSE: potassium cyanideABX Alphalyse: Lyses RBCs, releasing hemoglobin and WBCs. WBCs are counted by impedance.Waste
8 Daily Start UpTurn on instrument, once on, the start-up will begin automaticallyInstrument is performing a cleaning and rinsing cycle: detergent in the chamber is rinsed with diluentCycle lasts about 130 secondsAt cycle completion, background values will be displayed. These values must be less than the below:WBC < 0.3 x 103/mm3RBC < 0.02 x 106/mm3Hgb < 0.0 g/dLPlt < 10x 103/ mm3If the values are not within this range, another cycle will begin. If still not in range, the Micros will do another start-up. After 3 failed cycles, it will give a “start-up failed” message. Troubleshooting will now start.
9 Daily start-up con’t Run a primer sample Perform QC and evaluate Complete maintenance sheetIf all QC in, can continue with patient samples.
10 Running a Patient Press the ID button on the key pad Manually enter the ID. Use the up and down arrows to enter alphabetical characters. Please Enter after each entry.
11 Shutdown Hit the Stand by key, wait 1 minute Switch off instrument and printer.When the shutdown hit is pressed, the Micros 60 performs a complete cleaning with the Miniclean and puts the system into a Standby mode. Once this process is complete, the instrument can be switched off.