Presentation on theme: "Introduction to Instruments FUJI DRI-CHEM 4000i Scil Vet Focus 5 Cryostat for Frozen Sections 2010/12/06 Speaker ： Le-Shi Tsai."— Presentation transcript:
Introduction to Instruments FUJI DRI-CHEM 4000i Scil Vet Focus 5 Cryostat for Frozen Sections 2010/12/06 Speaker ： Le-Shi Tsai
Notice for the use of animal center Instrument Please attend illustration meeting before you begin operating instruments. If you do not participate in the illustration meeting, you may NOT use the instrument. (The meeting will be held 2-3 times per semester, and please pay attention to the animal center announcement.) Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation.
Charging methods InstrumentCharging methods FUJI DRI-CHEM 4000i Biochemistry Analyzer *Less than 3 tests : First hour - $ 400/hour Additional hours-$ 200 /hour *More than 3 tests : Each one more test, the first hour charge extra $100 Scil Vet Focus 5 Hematology Analyzer 1 test - $750 each additional test -$100 Leica CM3050 S Cryostat for Frozen Sections $150/0.5hr
Automatic detection 1. can be automatically diluted 2. A test only needs at least 10μL. （ Manual detection ）
Principle ： Colorimetric Spreading layer ： 1.ﬁltered out macromolecules (proteins, dyes, etc.) 2.evenly spread the fluid sample into the reaction layer β-D-glucose ＋ O2 ＋ H2O GOD D-gluconic acid ＋ H2O2 1,7-Dihydroxynaphthalene ＋ 4-aminoantipyrine ＋ H2O2 POD Red dye 505nm reflection density calibration curve glucose concentration
Principle ： Potentiometric generated voltage difference between ion selective electrodes applied to a reference solution and to the sample potentiometric difference between the sample and the reference solution is measured E = E0 + 2.303 * RT/Nf * Log ai ai = f * Ci Ci = Ion concentration
Introduction to Instrument The following animals can be detected: dog 、 rat 、 pig 、 rabbit 、 mouse 、 G. pig The following items can be detected: WBC 、 DC 、 RBC 、 HGB 、 HCT 、 MCV 、 MCH 、 MCHC 、 RDW 、 Retics 、 PLT 、 MPV 、 PCT Slide 002
Equipment main Instrument printer the kit waste pail the registration forms
Equipment the kit 1.Diluent 2.Clean Deproteinizer 3.Cellyse 1. r ed blood cell lysis 2. reagent to convert all hemoglobin to the stable cyanmethemoglobin form
Principles 感應區感應區 Diluent form a vortex Cells are injected into the core of a sheath flow and confined to a narrow single-file stream by hydrodynamic focusing(15μm). The cells are forced to pass through aperture in single file (one by one). 1.A distinguishing feature of blood cell types is the diameter of the cells. （ RBC 、 WBC 、 Platelet ） 2. WBCs are classified according to the characteristics of their cytoplasm and nucleus. 3. Cyanmethemoglobin can then be detected by a spectrophotometer set at 540 nm. The concentration of hemoglobin is then calculated from the optical density of the solution.
Specimen preparation Specimen requirements 1. Use the EDTA anticoagulant 3 ml tubes. 2. Specimen volume: At least 1.5 ml 3. Specimen volume are less than 1.5mL(at least 100 μL): Use small test tube containing a specimen and add EDTA as an anti-coagulant (Manual mode ). Preservation conditions Samples can be analyzed within 4 hour(room temperature). Interfering factors Measurement results are affected by hemolysis, chyle and clotted blood. DO NOT use clotted blood specimen.
Automatic Mode open the lid mix the specimen well put your sample into the specimen- container housing rack
Frozen Tissue Processing only use O.C.T as embedding medium Hard tissue using a dedicated film tissue process and cutting fixing and embedding freeze and slice stain mounting observe it under a microscope
The advantage of Leica CM3050 S It is ideal for undecalcified hard tissue : 1. can be set to any section thickness and section automatically 2. with a dedicated film, can avoid the hard tissue broken. with rough-cut function adjustable specimen temperature ： In accordance with the type of specimen to select the appropriate temperature. adjustable cutting speed
Specifications Specimen size Maximum 40mm Section thickness setting 0.5~300μm Sectioning speed 0.1~210mm/s Specimen temperature -10 ℃ ~-50 ℃
supplies you need to purchase ConsumablesSuitable specimens StageBuy different size according to the size of the specimen Disposable bladesGeneral tissue Ukrainian carbon blades Hard tissue Dedicated filmHard tissue