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Radiation-resistance? There's a Gene for That. New York City Wetware iGEM Team.

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Presentation on theme: "Radiation-resistance? There's a Gene for That. New York City Wetware iGEM Team."— Presentation transcript:

1 Radiation-resistance? There's a Gene for That. New York City Wetware iGEM Team

2 Extraordinary Biology Numbers Extremophile Numbers Extraordinary Synthetic Biology Numbers 130 Celsius 130 MPa 1.08-mega-base pair4 Days

3 Deinococcus radiodurans (D. rad) One to two hours of acute six to ten Gray radiation exposure. 12,000 Gray!!!!!

4 Life of the Cell

5 A Bad Day Base DamageSingle Strand BreakDouble Strand Break Active Site Damage

6 The Extraordinary Weapon (Kryptonite) 1.H 2 O  H· + OH· 2.OH· + OH·  H 2 O 2 3.Fe 2+ + H 2 O 2 → Fe 3+ + OH· + OH − Generation of Hydroxyl Radical via the Fenton Reaction

7 What Makes D. rad So Special? 1.Cellular Manganese-Iron Ratios 1.Neutralization of Superoxide Radicals 1.Superior DNA Repair Three Basic Approaches

8 What Makes D. rad So Special? 1.Cellular Manganese-Iron Ratios Three Basic Approaches

9 What Makes D. rad So Special? 2.Neutralization of Superoxide Radicals Three Basic Approaches

10 What Makes D. rad So Special? 3.Superior DNA Repair Three Basic Approaches recA Repair Protein

11 Making E. coli Radioresistant

12 Our Biobricks ruvA ruvB radA tpx ruvA

13 Future Applications Bioremediation

14 Future Applications Medical Applications

15 in vitro meat and moral traditions

16 Kosher Vegetarian Vegan

17 Legal Precedent: Gelatin

18 Legal Precedent: Rennet

19 Moral Considerations Ought a vegan or vegetarian to eat meat? Ought a kosher-observer to eat pork? Does cultivating meat without encountering an animal desensitize us as eaters? Does it undermine the relationship between ourselves and animals?

20 Our Sponsors


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