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ELF-Induced DNA Breaks Henry Lai Department of Bioengineering University of Washington Seattle, WA USA.

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Presentation on theme: "ELF-Induced DNA Breaks Henry Lai Department of Bioengineering University of Washington Seattle, WA USA."— Presentation transcript:

1 ELF-Induced DNA Breaks Henry Lai Department of Bioengineering University of Washington Seattle, WA USA

2 DNA Strand breaks -single and double strand breaks -mis-repair cumulative -affect cell functions; can lead to cancer and cell death

3 COMET ASSAY

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6 DNA strand breaks have been reported in different cell types after ELF EMF exposure. Ahuja (99)- human leukocytes Delimaris (06)- human lymphocytes Hong (05)- mouse sperm cells Ivancsit (02, 05)- human fibroblasts and melanocytes Ivancsit (03)-more pronounced effect observed in fibroblasts of older subjects. Jajte (01)- rat lymphocytes Lai and Singh (97, 04)- rat brain cells Li (01)- E. coli Svedenstal (99)- mouse brain cells Wolf (05)- HL-60 and rat fibroblasts Zmyslong (00, 04)- rat lymphocytes

7 ELF EMF affects DNA repair Robison (02) HL-60 –decrease Chow (00) – E. coli- increase- heat shock protein Schmitz (04)- Mouse brain and kidney cells- increase

8 ELF EMF genotoxic effects involve free radicals Effects blocked by free radical scavengers, enhanced by divalent metals, increase oxidative-damage in molecules Jaite (01) Lai and Singh (97) Li (01) Lourencini (00) Yokus (05) Wolf (05) Zmyslong (00, 04)

9 Electromagnetic fields iron mitochondria H2O2H2O2 OH Cellular damage THE FENTON REACTION

10 Intensity and duration of exposure interact. High intensity/short duration = Low intensity/long duration


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