2Objectives:Identify and locate laboratory safety devices in the classroom.Demonstrate proper/safe laboratory procedures.Identify and know the function of various laboratory equipment.Demonstrate proper laboratory techniques.
3Introduction Safety in the laboratory should be first and foremost Accidents in a laboratory may have the potential of resulting in serious consequences.Common laboratory accidents include cuts, burns, and the inhalation of volatile fumes.The occurrence of each of these accidents, and others, can be minimized if simple safety precautions are followed.
4When to wear goggles and aprons? If using chemicals and flames
6THINK!Accidents in the lab can be prevented!You are the most likely cause of an accident!
7When you first enter the lab… A. Go to lab and start experimentsB. try out your own experiments while you waitC. read the lab and wait for the teacher’s instructionsD. go pick up chemicals and practice the labANSWER: C
8Read Lab FirstRead the experimental procedure ahead of lab.
9Disposing of MatchesThe match should be quenched with water, NOT thrown into the waste crock directly.Fires may occur.
11What should I wear in the lab… if I am going to work with glassware?Goggles
12Closed Toed ShoesThe Safety Code of most schools will not approve of open foot ware; so avoid sandals or flip-flops
13NO FLOPPIES Avoid floppy garments; avoid things that dangle Avoid long, loose hair styles for the same reason.When Bunsen burners are in use, long hair sometimes catches on fire
14Where’s the Safety Equipment? Fire ExtinguishersFire BlanketEye-wash FountainFirst-Aid Kit
15Fire SafetySmall fires can be extinguished by covering them with a container, such as a beaker, to eliminate oxygen.Moderately sized fires require the use of a fire extinguisher.Large fires require the evacuation of the building and notifying the fire department.If your clothing catches fire, the best extinguisher is water from the safety shower.If a safety shower is not available, you can fall to the floor and cover yourself with a fire blanket.Make sure you are aware of the location of the safety shower and fire blanket prior to performing any experiments.Synthetic clothing fibers melt during combustion and produce hot liquids that stick to the skin. It is best to wear natural fibers, such as cotton, for fire safety when working in the laboratory.
16Fire Extinguishers the carbon dioxide extinguisher is red AVOID using a dry-chemical extinguisher on a fellow human being.the carbon dioxide extinguisher is redgeneral purpose extinguishers.
21EYE WASH If you get something from the lab in your eye— GET IT OUT IMMEDIATELY!Run some water through the eyewash fountain before you use it.Retract your eyelid (hold it open);Don’t squint—this restricts water access.Run fresh water over your eye for 20 minutes.Go to the school nurse immediately afterward.
22First Aid Kit These items may be used temporarily for small injuries (say, a cut finger) Go to the school nurse immediately afterward.Do not wait until the end of the day.
23First Aid KitHowever, if blood is in evidence-- STAY AWAY! Let the victim apply his/her own bandage.If blood is on the floor or lab bench, let trained personnel do the clean-up.
24FUME HOODUse the HOOD for reactions that give off vapors, especially smelly vapors.The draft of the HOOD will sweep away vapors so that the lab itself maintains reasonable air quality
25Safety Shower Shower should be used for dire EMERGENCY only! If you (or a lab mate) is spilled chemicals, position yourself (or your lab mate) under the safety shower.
26Safety ShowerPull the handle—a deluge of water will result.
27Safety ShowerThe safety shower should also be used if you suffer a massive spill of a dangerous chemical on yourself, and need to get it off rapidly.
28Broken GlassSweep it up right awayDon’t track in it all period.
30If you need to leave the lab table to go to the bathroom, A. Be sure someone is watching your lab stationB. shut off the gas valve and electrical equipment
31Is it OK to… A. eat in the lab B. put your head in the fume hood C. chew gum in the labD. drink in the labE. have horseplay in the labANSWER: NO
32What is the first aid step to do if you… Cut your fingerYour clothing caught on fireYou burned your fingerMercury ran out of a thermometerDon’t touch itStop drop and rollRun it under cold waterClean the wound, then use a band-aid
33All living organisms should be handled with… RESPECTThat means do not throw things into their homes.Do not tap on the aquaria.Do not throw them on other students.
34What should you bring back with you to the lab? A. Book BagB. Something to write withC. Lab sheetD. Biology BookE. LunchANSWER: B and C
35NO!!!!!!!!!!!!! No UNAUTHORIZED experiments! These are terrible dangers in unskilled hands.
36NO!!!!!!!!!!!!!!! No EATING or DRINKING in the LAB. A good practice is to assume everything in the lab is toxic.
37No!!!!!!!!!!!!!!!!! DO NOT drink from lab equipment. NOT EVEN distilled water.
38Pipets NOWhen using pipets, DO NOT suck the liquid into the pipet directly by mouth.
39Pipets YESDO use a rubber bulb to create a vacuum.
41Should you use glassware that is… ChippedDirtyCrackedANSWER? NO
42Testing of OdorsDo NOT smell the flask directly.
43Wafting YESIf you absolutely must test the odor, carefully waft the vapor from the flask toward your nose with your hand.Keep the flask quite distant from your face.
44HOT GLASSWARERemember NOT to hand the red-hot glass to another person, especially the teacher.
45Heating Test TubesMake sure that reaction tubes, e.g. test tubes, are not directed toward yourself or other persons.The chemicals may splatter out the tube.
46Aim away from you and all people! Heating Test TubesAim away from you and all people!
47Water to Acids NO DO NOT add WATER to CONCENTRATED ACID. The heat generated may cause splattering.
48Do as you ought’a - add Acids to waoughta (water) DO add ACID to WATER instead of the reverse order of addition.The heat generated will be less, but splattering still may occur.
49Working Safely in Biology Remember you are responsible for your own safety as well as that of your teacher, classmates, and any live animals you handle.
50Working Safely in Biology Scientists working in a laboratory or in the field are trained to use safe procedures.Laboratory work may involve contact with dangerous materials or organisms.
51Working Safely in Biology Follow safe practices.Study the safety rules.Read all the steps and safety precautions.Follow your teacher’s instructions and textbook directions exactly.If in doubt, ask your teacher for an explanation.Wash your hands thoroughly with soap and warm water after every scientific activity.
52Practice Quizzes Lab safety practice test Chemistry (and Biology) Safety Quiz
531. What best describes what a student should wear in the lab? a. Apron, closed toe shoes, gogglesb. Just gogglesc. Baggie clothes
542. It is the responsibility of the student to... a. Come prepared for the labb. Clean up all equipment and the lab table before the class is overc. Participate in the experimentd. All of the above
553. Which is permitted in the lab? a. your lab instructions, lab book, and writing utensilb. your lunch, Mrs. B said it was okc. your bottle of water
564. Where should you dispose of broken glass a. in the box by the teachers deskb. in any trash canc. in the sinkd. in the lab drawer
575. If you splash a chemical in your eye, you should a. rinse immediately with water at the eye wash station, then go to the nurseb. pat it dry with your sleeve, then rinse at your sink, then the eye wash stationc. tell Mrs. B and then go to the nursed. tell your lab partner
586. The proper way to detect the odor of a chemical is to a. waft- create a draft by waving your handb. place your nose 2 inches awayc. pour it in your hands and then smell itd. shake the container to release the vapors
597. It is ok use glassware that is: a. dirtyb. crackedc. chippedd. clean and dry and crack or chip free
608. Your lab partner just left to go to the bathroom, and you need additional supplies. You should a. Go ahead and get what you needb. Wait for your lab partner to return, so that items are not left unattended
619. All living organisms must be handled a. with respect and only with Mrs. B's permissionb. with a stick and glovec. only by the teacher
6210. Burned your finger on hot glass a. use the eye-wash stationb. use a band-aidc. rinse with cold waterd. use a fire extinguisher
6311. Your clothing caught on fire a. use the eye wash stationb. stop drop and roll/use fire blanketc. rinse with cold waterd. use a fire extinguisher
6412. Your lab directions catch on fire a. use the eye wash stationb. stop drop and roll/use fire blanketc. watch them burnd. use a fire extinguisher or fire blanket
6513. You cut your fingera. use the eye-wash stationb. use a band-aidc. rinse with cold waterd. use a fire extinguisher
6614. Your eyes are red and feeling irritated a. use the eye-wash stationb. use a band-aidc. rinse with cold waterd. use a fire extinguisher
6715. When done using a matcha. throw it in the bucket near Mrs. B's deskb. quench it with water, then throw it in the bucket near Mrs. B's deskc. quench it with water and then throw it in the sink, Mrs. B loves to cleand. leave it on the lab table until class is over
6816. If a friend gets cut and is bleeding a. help them immediatelyb. stay away, and let the friend apply his/her own bandage and trained personnel clean up the blood
6917. The most common accidents in the Biology lab are: a. firesb. slipping and fallingc. cuts and burns
7018. The fastest way to be removed from the lab is/are: a. horseplayb. not wearing gogglesc. opening the chemistry drawers and playing with the equipmentd. all of the above