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SMS 501 Biological Oceanography 16 September 2009 Lecture 5 Mary Jane Perry Maine Coastal Waters Conference 2009

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Presentation on theme: "SMS 501 Biological Oceanography 16 September 2009 Lecture 5 Mary Jane Perry Maine Coastal Waters Conference 2009"— Presentation transcript:

1 SMS 501 Biological Oceanography 16 September 2009 Lecture 5 Mary Jane Perry Maine Coastal Waters Conference October 28 (Wednesday, 0900) Issues? Car pooling *Essay 1 due next week *Concept map 1 the following week

2 SMS 501 Biological Oceanography 16 September 2009 Lecture 5 Mary Jane Perry This Week – Phytoplankton II Review of last week Assessing phytoplankton – as individuals or as mass Introduction to photosynthesis Pigments – photo-adaption and accessory pigments Regulation of photosynthesis Rates of primary productivity Sverdrup concept of bloom initiation

3 Review of Phytoplankton I Phytoplankton are little factories. – transform electromagnetic energy into chemical energy. – transform dissolved nutrients & CO 2 into organic particles. They are incredibly diversity, both taxonomically and functionally. – taxonomically –> 2 Super Kingdoms (Eubacteria & Eukaryotes) – size (from ~ 1 µm to 100 µm+); regional variation in size spectrum – chemical composition (fatty acids, pigments, etc.) – nutrient cycling (Si – only diatoms, N 2 fixation – only cyano, etc.) Growth rate – cells increase in size (little factories) and divide. Cell number at t X = (cell number at t 0 * 2 kt ) = (cell number at t 0 * e µt ) Growth rate is regulated by temperature, light, and nutrients. Satellite ocean color – reflected light; empirical relationship w/chl.

4 Phytoplankton abundance – how to assess Two historical approaches: as individuals and as mass (average or ‘bulk’ measure) How to reconcile the two? – for example, number of cells vs. chlorophyll How are the two measures used to normalize rates? How are the two measures used in models and for prediction?

5 microscopic identification : * microscopic identification to some taxonomic level (slow) – light microscope, if cells are large enough – SEM for small cells, not quantitative – epifluorescence (autofluorescence, stains) * image analysis, classification of types by pattern recognition other (can be fast or faster than microscope) : * Coulter Counter – electronic, change in electrical impedance, but counts all particles, not just phytoplankton – size spectra * flow cytometry: light scattering and fluorescence – optical classification of particles (typically not species) * molecular probes (qualitative) – in situ hybridization & stain – couple with image analysis or flow cytometry Measure phytoplankton as individuals with microscope (more taxonomic information) or other technologies (less taxonomic information).

6 Synechococcus with phycoerythrin (orange) & chl fluorescence (red) Chloroplasts (red) in colony of diatom Thalassionema ; red chl fluorescenc and blue DAPI nuclear stain. Epifluorescence microscopy Microscopy of cells (working toward automation w/ image classification). Note morphological variation in one species, Dinophysis acuminata.

7 Sheldon et al., L&O 17: 327 Coulter Counter – electronic particle counter (counts all particles, not just phytoplankton). Particle size spectrum (~ 1 µm to about 500 µm).

8 Single cell – flow cytometry Flow cytometry: – single cell or particle analysis – multiple optical discriminants (scatter and fluorescence) (from Sosik & Olson)

9 so-called “bulk” measures of phytoplankton biomass elements – C, N, P, Si (problem: elements are not necessarily unique to phytoplankton; most elements are found in all living organisms) pigments, primary chlorophyll a – found only in photosynthetic organisms; some accessory pigments unique to specific taxon. (µg L -1 or mg m -3 ; mg m -2 for integrating water column) –> apply chlorophyll–to–C ratio (problem: ratio varies with light conditions of growth) unique chemicals – taxon specific (fatty acids; sterols {dino- flagellates}’ lipids {alkenones – coccolithophorids}; etc.) optics – satellite remote sensing, absorption and scattering Phytoplankton measurement as mass (biomass) (biomass is ‘state variable’ – in contrast to ‘rate’ variable) bio – biological or living; mass – grams (in MKS system - meter, kg, sec)

10 ‘Chlorophyll biomass’ – proxy for phytoplankton carbon: extract of chlorophyll in lab & in vivo fluorescence in the water’ Extract chlorophyll a: * filter cells * extract 90% acetone * blue light source, red light emission * concentration is proportional to red light emitted cell carbon chlorophyll a Irradiance of growth Chlorophyll is unique to photosynthetic organisms. One application is to use chlorophyll to obtain phytoplankton carbon. Multiply by carbon–to–chlorophyll ratio (g/g). Ratio typically (O(15 – 50 g/g)) (but can exceed 50 g/g under high light or low nutrients).

11 Chlorophyll a is used as proxy for phytoplankton biomass because it is ONLY found in photosynthetic organisms (exception – mixotrophic species & while being digested in heterotrophs) In vivo chlorophyll a fluorescence provides information on vertical and horizontal distribution of phytoplankton; (data collected with underwater glider in Monterey Bay) Extracted chlorophyll a (µg/L) used in iron-fertilization experiments to assess bulk phytoplankton growth response to Fe.

12 Combined information from extracted chlorophyll AND flow cytometric classification (based on optical signals). Tarran et al DSR II 53: 1516 ATM 13 cruise from 50ºN to 50ºS 7-m pigment (solid line) 100-m integrated pigment (dotted) Subgroups converted to C biomass.(Note change in scales.) Note Mauritanian upwelling – 20ºN.

13 Regional biomass differences as chlorophyll a (mg chl a m -3 ) Oligotrophic gyres (mostly small ultra and picoplankton) Coastal upwelling 2-16 (nano and larger netplankton) Coastal shelves (seasonal: spring net & nano, summer picoplankton) Equatorial Pacific upwelling (small picoplankton) (seasonality; variance high in coastal waters; eutrophication - higher) SeaWiFS chl

14 Information from phytoplankton concentration (typically chlorophyll biomass – state variable) is applied to rate measurements (rate variable) to make rates of primary productivity portable. Satellite chlorophyll concentrations

15 Photosynthesis – process of conversion of energy of electro- magnetic radiation (light) to chemical energy (ultimately, carbon). Pigments (chlorophyll a and accessory pigments are essential in this process – they absorb light. CO 2 + H 2 O -----> -(CH 2 O)- + O 2 Primary product of ‘light reactions’ are ATP & NADPH (high energy reductants). Initial product of ‘synthetic reactions’ or Calvin cycle is 3C sugar. Final products that are synthesized include proteins, lipids, nucleic acids, etc.; complex molecules require additional energy to synthesize. Photosynthetic Quotient (= ratio between O 2 evolved and C fixed) varies with types of compounds synthesized; varies from ~1.0 to 1.5

16 Functional response of photosynthesis to light. This is P vs. E curve. Photosynthetic rates are normalized to chl a. P max is maximal rate;  is slope (rate/irradiance); E k – transition from light limited photosynthesis to light saturated photosynthesis; R is phytoplankton respiration. What is gross vs. net photosynthesis? P = P max ( 1 – e (  E/Pmax) ) E k =  /P max

17 irradiance (E) zz ln irradiance (E) E = Eo e -Kz Units of irradiance:  mol photon m -2 s -1 or mol photon m -2 d -1 (rather than watts m -2 ) K, diffuse attenuation coefficient (m -1 ) z, depth (m) E, irradiance (  moles photon m -2 s -1 ) Euphotic zone - how to define? 1% light level vs. absolute value Light regulates photosynthesis Light decreases exponentially with depth.

18 What are implications for seasonality in photosynthesis? 1. total irradiance –> euphotic zone depth 2. day length (sp. effect) 3. solar heating –> stratification –> mixed layer depth Seasonality in light equator pole

19 E = Eo e -Kz PAR (400–700 nm) vs. spectral light, E( ) z ln irradiance (E) K ( ) varies for different types of water masses

20 Pigments capture energy from light Chlorophyll b or cPhotosynthetic carotenoidsPhycobilipigments Chlorophyll a multiple pigments –> broader spectral window (chl a & b in land plants) – of light must match  of pigment absorption

21 Advantage of multiple pigments – broadening of spectral window for absorption (and growth). A mismatch between absorption and spectrum of light means no photosynthesis for that species.

22 HPLC allows resolution of individual pigments; information can be used for taxonomic classification.

23 Chem Tax (requires training) See Miller Table 2.2

24 Both rates of photosynthesis and pigment concentration per cell are regulated by irradiance chl a/cell growth irradiance

25 Phytoplankton can photo-adapt to high or low light 1. At low light:more light-harvesting pigments 2. At high light:fewer light-harvesting pigments but more photoprotective pigments (carotenoids). chl a/cell irradiance at which cells are grown

26 Change in pigmentation in high or low light changes photosynthetic rate as a function of irradiance. 1.Low-light grown cells have more light-harvesting pigments and have higher rates of photosynthesis at low light. 2.High-light grown cells have fewer light-harvesting pigments. chl a/cell low-light grown PS rate  pg C cell -1 h -1  High-light grown irradiance

27 Consequence of photo-adaptation A cell does better at low light (synthesizes more pigments/cell) than it would without ability to change pigmentation/cell. By reducing pigments/cell at high light, a cell minimizes photo- inhibition due to photo-damage at high light. chl a/cell low E PS rate  pg C cell -1 h -1  hi E irradiance growth rate irradiance Down-side for oceanographers, is that the C-to-Chl ratio is not a constant but varies by factor ~3-4.

28 Photosynthesis is a function of irradiance P = P max ( 1 – e (  E/Pmax) ) Photosynthetic rate is typically normalized to chlorophyll concentration to make the measurement ‘portable’ Carbon–to–chlorophyll ratio changes with growth irradiance Light is attenuated exponentially as a function of depth; spectral attenuation varies in different water bodies. Photosynthetic accessory pigments enable phytoplankton to absorb broad spectrum of wavelengths (vs. land plants) How is photosynthetic rate measured in the ocean?

29 14 CO 2 + H 2 O ---> -( 14 CH 2 O)- + O 2 14 C added = 14 C in cells on filter total alkalinity total Carbon fixed (total DIC ) Photosynthesis can be measured as oxygen evolution (often tough to make good oxygen measurement for low phytoplankton biomass) or as 14 C incorporation (Steeman Nielsen, 1952) Units per volume are mg C m -3 or mmol C m -3 (integrated: mg C m -2 or mmol C m -2 ). Make rates portable by normalizing to something (typically, chlorophyll a concentration).

30 Real data from HOTS: Pattern of photosynthetic rates as function of depth (O 2 and 14 C) Photosynthetic quotient O 2 evolved /C fixed Why does quotient vary? DSR I 51: 1563

31 Relationship between biomass (Chl a) and productivity? Portability of rates: use rate information normalized to biomass PS = pigment concentration * rate per pigment (also include some type of light function) Prog. Ocean. 65:159 – Arabian Sea A. Thomas, UMaine

32 Large number of models to go from satellite chlorophyll (biomass) to primary productivity (rate)

33 Use of satellite data allows one to ask questions – Does ocean productivity change with climate cycles? or Is there a secular trend in response to climate change? El Nino La Nina

34

35 Regional patterns of PP, derived primarily with 14 C Table from Barber and Hilting in Williams et al. (2002) (see Søndergaard for global estimates) Biomass Regional differences: (mg Chl a m -3 ) Oligotrophic gyres Coastal upwelling 2-16 Coastal shelves Equatorial Pacific upwelling (variance is highest in coastal waters) Productivity Regional differences: (gC m -2 y -1 ) Oligotrophic gyres 50 Coastal shelves 200 Upwelling (also will be seasonal and daily variability, etc.) Regional & global estimates of PP: Biomass * PP rate/biomass * area

36 Important Concepts Production is fixation of carbon; productivity is rate of production 1. gross PP: total rate of phytoplankton fixation of carbon 2. net PP: rate of phytoplankton fixation of carbon minus phytoplankton consumption* of carbon (via respiration at night) 3. net net PP or community net PP: rate of net phytoplankton fixation minus consumption (grazing by protoza and zooplankton; metabolism of DOC by bacteria). Recall dP = P(growth – loss) dt 4. new PP: exportable production to deep water column or fish (need to define boundary conditions) NB: Time period for measuring & integrating makes a difference: community net and new PP will be different if PP is integrated per hour vs. per day or per year.WHY?

37 D Other ways (than 14 C) to estimate primary productivity. For example, oxygen evolution and accumulation for net community PP. (longer term measurement than 14 C) ARGO floats near Hawaii. Riser & Johnson Nature 451: 323

38 D Other ways (than 14 C) to estimate primary productivity. Mass balance – Redfield ratio for C:N O 2 and nitrate from ISUS on float agree; but less POC standing stock than POC produced. Lagrangian mixed-layer float, subpolar N. Atlantic spring bloom. D’Asaro et al., unpub.

39 irradiance (E) z Draw photosynthesis vs. depth

40 irradiance (E) z Photoinhibition – only if surface light is high

41 Sverdrup and critical depth Relationship between gain and loss terms: dP = P (gain – loss) dt When phytoplankton gain > loss terms, bloom occurs. Could be due to more light, reduced mixing (stratification), etc. Critical depth: Depth at which integrated water column gain = integrated water column loss (photosynthesis = respiration). If mixed layer is > critical depth, no bloom. If mixed layer is < critical depth, bloom. Compensation depth: Depth at which photosynthesis = respiration.

42 Unanswered questions


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