1. 2007 IUFRO All Division 5 Conference, Taipei 5.07.00 B Energy from biomass / Chemicals from wood The antioxidant activity and protective effect of extracts from the stem of Vitis kelungeusis on carbon tetrachloride-induced acute liver injury in mice Department of forestry, National Chung-Hsing University Kai-Chung Chang Kai-Chung CHANG, Lie-Fen SHYUR, Chi-Chang HUANG, Shih-Chang CHIEN and Jyh-Horng WU

2. Experimental and methods The objective of this study is to investigate and identify the bioactive secondary metabolisms from wild grape. In vitro assay DPPH radical scavenging assay Total phenolic contents In vivo assay i. p. Extracts (10 mg/Kg of BW, i. p.) i. p. Silymarin (50 mg/Kg of BW, i. p.) i. p. Vehicle (Negative control, i. p.) i. p 20% CCl 4 in olive oil (40 µL/kg of BW, i. p.) -2 d -1 d 0 6 hr Scarified Male ICR mice 6 weeks old Kai-Chung Chang

3. Concentration (µg/mL) Inhibition (%) Crude extract EtOAc BuOH Water Total phenolic contents (mg GAE/g) Fractions DPPH radical scavenging activity of V. kelungeusis extracts Total phenolic contents of V. kelungeusis extracts Results Kai-Chung Chang

4. In vitro assay The crude extract and its derived soluble fractions showed excellent antioxidant activities.Of these the BuOH fraction was the strongest one. In vivo assay Mice pretreated with crude extract or BuOH fraction (10 mg/kg/day, i.p.) for 3 days, remarkably prevented the elevation of serum AST and ALT activities on CCl 4 -induced acute liver injury. Results Resveratrol derivatives (+)-Balanocarpol 0.90% (-)-ε-Viniferin 6.34% New compound 0.39% New compound 2.68% Kai-Chung Chang

5. Thanks for your attention Kai-Chung Chang